Key features and details
- Rabbit polyclonal to Natriuretic Peptide Receptor A/GC-A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Natriuretic Peptide Receptor A/GC-A antibody
See all Natriuretic Peptide Receptor A/GC-A primary antibodies
DescriptionRabbit polyclonal to Natriuretic Peptide Receptor A/GC-A
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- This antibody gave a positive signal in Kidney (Human) Tissue Lysate - adult normal tissue
Previously labelled as Natriuretic Peptide Receptor A.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab40817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 119 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionReceptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
Sequence similaritiesBelongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 1 guanylate cyclase domain.
Contains 1 protein kinase domain.
modificationsPhosphorylation of the protein kinase-like domain is required for full activation by ANP.
- Information by UniProt
- ANP-A antibody
- ANPa antibody
- ANPR-A antibody
Anti-Natriuretic Peptide Receptor A/GC-A antibody (ab40817) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 119 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab40817 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40817, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab40817 has not yet been referenced specifically in any publications.