Recombinant
RabMAb

Recombinant Anti-Natriuretic Peptide Receptor B antibody [EPR5719(2)] (ab139188)

Overview

  • Product name

    Anti-Natriuretic Peptide Receptor B antibody [EPR5719(2)]
    See all Natriuretic Peptide Receptor B primary antibodies
  • Description

    Rabbit monoclonal [EPR5719(2)] to Natriuretic Peptide Receptor B
  • Host species

    Rabbit
  • Specificity

    The immunogen for this product shares 95% homology with NPR-A
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Natriuretic Peptide Receptor B aa 800-900. The exact sequence is proprietary.
    (Peptide available as ab150296)

  • Positive control

    • Human artery, fetal kidney, fetal brain, Mouse brain, Mouse heart, Rat brain, and Rat heart tissue lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab139188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 117 kDa.Can be blocked with Natriuretic Peptide Receptor B peptide (ab150296).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.
    • Involvement in disease

      Defects in NPR2 are the cause of acromesomelic dysplasia Maroteaux type (AMDM) [MIM:602875]. Acromesomelic chondrodysplasias are rare hereditary skeletal disorders characterized by short stature, very short limbs, and hand/foot malformations. The severity of limb abnormalities increases from proximal to distal with profoundly affected hands and feet showing brachydactyly and/or rudimentary fingers (knob-like fingers). AMDM is an autosomal recessive form characterized by axial skeletal involvement with wedging of vertebral bodies. In AMDM all skeletal elements are present but show abnormal rates of linear growth.
    • Sequence similarities

      Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
      Contains 1 guanylate cyclase domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylation of the protein kinase-like domain is required for full activation by CNP.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AMDM antibody
      • ANP-B antibody
      • ANPB antibody
      • ANPR-B antibody
      • ANPRB antibody
      • ANPRB_HUMAN antibody
      • Atrial natriuretic peptide B type receptor antibody
      • Atrial natriuretic peptide receptor 2 antibody
      • Atrial natriuretic peptide receptor type B antibody
      • Atrionatriuretic peptide receptor B antibody
      • GC-B antibody
      • GCB antibody
      • Guanylate cyclase B antibody
      • GUC 2B antibody
      • GUC2B antibody
      • GUCY2B antibody
      • Natriuretic peptide receptor B antibody
      • Natriuretic peptide receptor B/guanylate cyclase B antibody
      • NPR-B antibody
      • Npr2 antibody
      • NPRB antibody
      • NPRBi antibody
      see all

    Images

    • All lanes : Anti-Natriuretic Peptide Receptor B antibody [EPR5719(2)] (ab139188) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : Human artery lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 117 kDa

    • All lanes : Anti-Natriuretic Peptide Receptor B antibody [EPR5719(2)] (ab139188) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 117 kDa

    References

    ab139188 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Question
    Answer

    Thank you for your inquiry. This antibody targets an intracellular portion of the protein. If you have any other questions or concerns please feel free to contact us.

    Read More

    Answer


    The lab let me know that when they tested ab139188 in IHC-P, they used a wide variety of normal and cancer tissue arrays (usually human only). They did not see any staining.

    Read More

    Answer

    Thank you for contacting us. Any anti-rabbit antibody should work well with this product. Generally I recommend using a pre-absorbed antibody such as ab7078. Pre-adsorbed secondary antibodies are less likely to interact with endogenous

    Read More

    Question
    Answer

    Thank you for your patience.

    I have heard back from the lab that the blocking peptide for ab116680 has been added to the catalog as ab151530: https://www.abcam.com/natriuretic-peptide-receptor-a-ab151530.html

    The blocking peptide for ab139188 has been added as ab150296: https://www.abcam.com/index.html?datasheet=150296

    Both items will be in stock in 1-2 weeks once an order is placed.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question
    Answer

    Thank you for your patience.

    The immunogen peptides for ab116680 and ab139188 would be available, and I have requested that they will be added to the catalog. I will let you know when I have more information regarding catalog number, prize etc.

    I hope this information is already of some help to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question
    Answer

    Thank you for your phone call.

    The UniProt database entry for Natriuretic Peptide Receptor A is as follows: http://www.uniprot.org/uniprot/P18293

    For ab79164, the immunogen peptide is in the catalog as product ab95057.
    For the other 2 antibodies I have inquired with the lab and am waiting to hear back from them. I will let you know when I do.

    The discount code for trying ab79164 in mouse is as follows:
    DISCOUNT CODE: xxx
    Expiration date: xxx
    Value: xxx

    I am very pleased to hear you would like to accept our offer and test ab79164 in mouse. This code will give you xxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up