Anti-Natriuretic Peptide Receptor C antibody (ab14355)

Overview

  • Product name
    Anti-Natriuretic Peptide Receptor C antibody
    See all Natriuretic Peptide Receptor C primary antibodies
  • Description
    Rabbit polyclonal to Natriuretic Peptide Receptor C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, ICC, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide:

    QEEGLHSIYSFDET

    conjugated to poly Lysin, corresponding to amino acids 199-212 of Human Natriuretic Peptide Receptor C.

Properties

Applications

Our Abpromise guarantee covers the use of ab14355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/800. PubMed: 19878700
ICC 1/800.
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 64 kDa.

Target

  • Function
    Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.
  • Sequence similarities
    Belongs to the ANF receptor family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANP C antibody
    • ANP-C antibody
    • ANPC antibody
    • ANPR-C antibody
    • ANPRC antibody
    • ANPRC_HUMAN antibody
    • Atrial natriuretic peptide C type receptor antibody
    • Atrial natriuretic peptide clearance receptor antibody
    • Atrial natriuretic peptide receptor 3 antibody
    • Atrial natriuretic peptide receptor type C antibody
    • Atrionatriuretic peptide receptor C antibody
    • C5orf23 antibody
    • FLJ14054 antibody
    • GUCY2B antibody
    • MGC22189 antibody
    • Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) antibody
    • Natriuretic peptide receptor C/guanylate cyclase C antibody
    • NPR 3 antibody
    • NPR C antibody
    • NPR-C antibody
    • Npr3 antibody
    • NPRC antibody
    see all

Images

  • ICC/IF image of ab14355 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14355, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
See all 5 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. Your question is more difficult than I expected it to be! We have a menu in our Product Browser (located in the upper left-hand corner of our homepage: Tags and Cell Markers/Subcellular Markers/Plasma Membrane) that gives some ideas for antibodies against membrane proteins that may be found in ATDC5 but few that fit your criteria. The pan-cadherin antibodies may be your best choice though they have not been tested for flow cytometry. Antibody ab6529 has been tested in immunocytochemistry, is raised against an intracellular portion of the protein, and reacts with the mouse protein. I expect it can be used for flow cytometry if the cells are fixed and permeabilized, but we cannot guarantee this application. For an antibody that recognizes an extracellular region, the antibodies against the integrins may be useful. Typing "integrin" brings up a list of antibodies that can be filtered by species and application. The ones that have been used for functional studies (FuncS), blocking (BL), or cellular activation will be directed against a portion of the integrin that is extracellular, for instance ab25198 (CD103) or ab36436 (CD61). But these integrins are expressed to different degrees on cell types and have mostly been studied on leukocytes. Various receptors may be useful; a literature search on ATDC5 gave the following link: http://ajplung.physiology.org/cgi/content/full/274/3/L425 The article mentions the natriuretic peptide receptor on ATDC5. We carry several NPR antibodies which can be brought up in a list by searching with our browser for NPR. None have been tested in flow cytometry but ab14355 is raised against an extracellular portion of NPR-C, and ab37620 is raised against a cytoplasmic portion of NPR-B. But this has only been tested for Western blotting, ELISA, and IHC. I hope this helps. Please let me know if you have any questions.

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