Anti-NPR-C antibody (ab97389)
Key features and details
- Rabbit polyclonal to NPR-C
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-NPR-C antibody
See all NPR-C primary antibodies -
Description
Rabbit polyclonal to NPR-C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human NPR-C aa 265-514.
Database link: NP_000899 -
Positive control
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lines IHC-P: U87 xenograft ICC/IF: Hela cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 60 kDa.
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IHC-P | (2) |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 60 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity. -
Sequence similarities
Belongs to the ANF receptor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 281358 Cow
- Entrez Gene: 4883 Human
- Entrez Gene: 18162 Mouse
- Entrez Gene: 25339 Rat
- Omim: 108962 Human
- SwissProt: P10730 Cow
- SwissProt: P17342 Human
- SwissProt: P70180 Mouse
see all -
Alternative names
- ANP C antibody
- ANP-C antibody
- ANPC antibody
see all
Images
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Anti-NPR-C antibody (ab97389) at 1/10000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
ab97389, at a 1/200 dilution, staining NPR-C in paraformaldehyde fixed HeLa cell by Immunofluorescence.
The lower image (Panel 2) was merged with DNA probe. -
ab97389, at a 1/500 dilution, staining NPR-C in paraffin embedded U87 xenograft by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab97389 has been referenced in 4 publications.
- Zhang YL et al. Metformin combats high glucose-induced damage to the osteogenic differentiation of human periodontal ligament stem cells via inhibition of the NPR3-mediated MAPK pathway. Stem Cell Res Ther 13:305 (2022). PubMed: 35841070
- Sun M et al. PLXND1-mediated calcium dyshomeostasis impairs endocardial endothelial autophagy in atrial fibrillation. Front Physiol 13:960480 (2022). PubMed: 36017337
- Zayed MA et al. Natriuretic Peptide Receptor-C is Up-Regulated in the Intima of Advanced Carotid Artery Atherosclerosis. J Med Surg Pathol 1:N/A (2016). IHC-P ; Human . PubMed: 27547837
- Peake N et al. Natriuretic peptide receptors regulate cytoprotective effects in a human ex vivo 3D/bioreactor model. Arthritis Res Ther 15:R76 (2013). WB, IF . PubMed: 23883591