Recombinant Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20247] to Natriuretic peptides A
- Suitable for: WB, IHC-P, IHC-Fr, IP, mIHC
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Natriuretic peptides A antibody [EPR20247]
See all Natriuretic peptides A primary antibodies -
Description
Rabbit monoclonal [EPR20247] to Natriuretic peptides A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, IP, mIHCmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal heart and rat heart tissue lysates. IHC-P: Human heart, rat auricle and rat ventricle tissues. IHC-Fr: Rat heart tissue. IP: Rat heart lysate. mIHC: Human cardiac muscle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20247 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 16 kDa.
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IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
1/100.
Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). |
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IP |
1/30.
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mIHC |
Use a concentration of 0.241 µg/ml.
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Notes |
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WB
1/1000. Predicted molecular weight: 16 kDa. |
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/100. Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). |
IP
1/30. |
mIHC
Use a concentration of 0.241 µg/ml. |
Target
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Function
Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Involvement in disease
Atrial standstill 2
Atrial fibrillation, familial, 6 -
Sequence similarities
Belongs to the natriuretic peptide family. -
Post-translational
modificationsCleaved by CORIN upon secretion to produce the functional hormone.
Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4878 Human
- Entrez Gene: 24602 Rat
- Omim: 108780 Human
- SwissProt: P01160 Human
- SwissProt: P01161 Rat
- Unigene: 75640 Human
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Alternative names
- ANF antibody
- ANF_HUMAN antibody
- ANP antibody
see all
Images
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Fluorescence multiplex immunohistochemical analysis of the human cardiac muscle (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-delta Sarcoglycan (ab137101, red; Opal™690), anti-Titin (ab307446, green; Opal™520) and anti-Natriuretic peptides A (ab209232, magenta; Opal™570) on human cardiac muscle. Panel B: anti-Titin displayed nucleus and cytoplasm expression. Panel C: anti-Natriuretic peptides A displayed granular cytoplasmic expression. Panel D: anti-delta Sarcoglycan displayed membrane expression. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
The section was incubated in three rounds of staining: in the order of ab137101 at 1/1000 (1.043 μg/ml) dilution, ab307446 at 1/500 (0.95 μg/ml) dilution, and ab209232 at 1/3000 (0.241 μg/ml) dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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All lanes : Anti-Natriuretic peptides A antibody [EPR20247] (ab209232) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 2 seconds; Lane 2: 15 seconds.
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Immunohistochemical analysis of paraffin-embedded human heart tissue labeling Natriuretic peptides A with ab209232 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Granularly cytoplasmic and perinuclear staining on human heart (PMID: 2942710, PMID: 1824903).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Natriuretic peptides A with ab209232 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Cytoplasmic staining on the auricula of rat heart (PMID: 2942710, PMID: 1824903).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat auricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Granularly cytoplasmic and perinuclear staining on rat auricle (PMID: 2942710, PMID: 1824903).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat ventricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Weak staining on rat ventricle (PMID: 2942710, PMID: 1824903).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Natriuretic peptides A was immunoprecipitated from 0.35 mg of rat heart lysate with ab209232 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab209232 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Rat heart lysate 10 µg (Input).
Lane 2: ab209232 IP in rat heart lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209232 in rat heart lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab209232 has been referenced in 3 publications.
- Cho S et al. Nelumbo nucifera Receptaculum Extract Suppresses Angiotensin II-Induced Cardiomyocyte Hypertrophy. Molecules 24:N/A (2019). PubMed: 31027372
- Chen K et al. Protective effect of histone methyltransferase NSD3 on ISO-induced cardiac hypertrophy. FEBS Lett 593:2556-2565 (2019). PubMed: 31254363
- Zhang B et al. C1q-TNF-related protein-3 attenuates pressure overload-induced cardiac hypertrophy by suppressing the p38/CREB pathway and p38-induced ER stress. Cell Death Dis 10:520 (2019). PubMed: 31285424