Recombinant
RabMAb

Recombinant Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20247] to Natriuretic peptides A - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free
    See all Natriuretic peptides A primary antibodies

  • Description

    Rabbit monoclonal [EPR20247] to Natriuretic peptides A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant full length protein within Human Natriuretic peptides A aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P01160

  • Positive control

    • IHC-P: Human heart tissue.

  • General notes

    Ab225873 is the carrier-free version of ab209232. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab225873 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab225873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
IP 1/30.

Target

  • Function

    Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

  • Involvement in disease

    Atrial standstill 2
    Atrial fibrillation, familial, 6

  • Sequence similarities

    Belongs to the natriuretic peptide family.

  • Post-translational
    modifications

    Cleaved by CORIN upon secretion to produce the functional hormone.
    Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • ANF antibody
      • ANF_HUMAN antibody
      • ANP antibody
      • ATFB6 antibody
      • Atrial natriuretic factor antibody
      • Atrial natriuretic peptide antibody
      • Atriopeptin antibody
      • Cardiodilatin antibody
      • Cardiodilatin related peptide antibody
      • Cardiodilatin-related peptide antibody
      • Cardionatrin antibody
      • CDD ANF antibody
      • CDD antibody
      • CDD-ANF antibody
      • CDP antibody
      • Natriuretic peptide A antibody
      • Natriuretic peptide precursor A antibody
      • NPPA antibody
      • PND antibody
      • Prepronatriodilatin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)

      Immunohistochemical analysis of paraffin-embedded rat auricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Granularly cytoplasmic and perinuclear staining on rat auricle (PMID: 2942710, PMID: 1824903).

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)

      Immunohistochemical analysis of paraffin-embedded rat ventricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Weak staining on rat ventricle (PMID: 2942710, PMID: 1824903).

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
      Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Natriuretic peptides A with ab209232 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Cytoplasmic staining on the auricula of rat heart (PMID: 2942710, PMID: 1824903).

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
      Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)

      Natriuretic peptides A was immunoprecipitated from 0.35 mg of rat heart lysate with ab209232 at 1/30 dilution.

      Western blot was performed from the immunoprecipitate using ab209232 at 1/500 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Rat heart lysate 10 µg (Input).

      Lane 2: ab209232 IP in rat heart lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209232 in rat heart lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)

      Immunohistochemical analysis of paraffin-embedded human heart tissue labeling Natriuretic peptides A with ab209232 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Granularly cytoplasmic and perinuclear staining on human heart (PMID: 2942710, PMID: 1824903).

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab225873? Please let us know so that we can cite the reference in this datasheet.

    ab225873 has not yet been referenced specifically in any publications.

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