Recombinant
RabMAb

Recombinant Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)

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Overview

  • Product name

    Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free
    See all Natriuretic peptides A primary antibodies

  • Description

    Rabbit monoclonal [EPR20247] to Natriuretic peptides A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant full length protein within Human Natriuretic peptides A aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P01160

  • Positive control

    • IHC-P: Human heart tissue.

  • General notes

    Ab225873 is the carrier-free version of ab209232. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab225873 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab225873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
IP 1/30.

Target

  • Function

    Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

  • Involvement in disease

    Atrial standstill 2
    Atrial fibrillation, familial, 6

  • Sequence similarities

    Belongs to the natriuretic peptide family.

  • Post-translational
    modifications

    Cleaved by CORIN upon secretion to produce the functional hormone.
    Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • ANF antibody
      • ANF_HUMAN antibody
      • ANP antibody
      • ATFB6 antibody
      • Atrial natriuretic factor antibody
      • Atrial natriuretic peptide antibody
      • Atriopeptin antibody
      • Cardiodilatin antibody
      • Cardiodilatin related peptide antibody
      • Cardiodilatin-related peptide antibody
      • Cardionatrin antibody
      • CDD ANF antibody
      • CDD antibody
      • CDD-ANF antibody
      • CDP antibody
      • Natriuretic peptide A antibody
      • Natriuretic peptide precursor A antibody
      • NPPA antibody
      • PND antibody
      • Prepronatriodilatin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)

      Immunohistochemical analysis of paraffin-embedded rat auricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Granularly cytoplasmic and perinuclear staining on rat auricle (PMID: 2942710, PMID: 1824903).

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)

      Immunohistochemical analysis of paraffin-embedded rat ventricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Weak staining on rat ventricle (PMID: 2942710, PMID: 1824903).

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Natriuretic peptides A with ab209232 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Cytoplasmic staining on the auricula of rat heart (PMID: 2942710, PMID: 1824903).

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).

    • Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
      Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)

      Natriuretic peptides A was immunoprecipitated from 0.35 mg of rat heart lysate with ab209232 at 1/30 dilution.

      Western blot was performed from the immunoprecipitate using ab209232 at 1/500 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Rat heart lysate 10 µg (Input).

      Lane 2: ab209232 IP in rat heart lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209232 in rat heart lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)

      Immunohistochemical analysis of paraffin-embedded human heart tissue labeling Natriuretic peptides A with ab209232 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Granularly cytoplasmic and perinuclear staining on human heart (PMID: 2942710, PMID: 1824903).

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).

    References

    ab225873 has not yet been referenced specifically in any publications.

    Publishing research using ab225873? Please let us know so that we can cite the reference in this datasheet.

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