Recombinant Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.com/ps/products/225/ab225873/Images/ab225873-324891-anti-natriuretic-peptides-a-antibody-epr20247-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20247] to Natriuretic peptides A - BSA and Azide free
- Suitable for: WB, IHC-P, IHC-Fr, IP
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free
See all Natriuretic peptides A primary antibodies -
Description
Rabbit monoclonal [EPR20247] to Natriuretic peptides A - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, IPmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human heart tissue.
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General notes
ab225873 is the carrier-free version of ab209232.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20247 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IHC-Fr |
Use at an assay dependent concentration.
Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). |
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| IP |
1/30.
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| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
Use at an assay dependent concentration. Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). |
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IP
1/30. |
Target
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Function
Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Involvement in disease
Atrial standstill 2
Atrial fibrillation, familial, 6 -
Sequence similarities
Belongs to the natriuretic peptide family. -
Post-translational
modificationsCleaved by CORIN upon secretion to produce the functional hormone.
Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4878 Human
- Entrez Gene: 24602 Rat
- Omim: 108780 Human
- SwissProt: P01160 Human
- SwissProt: P01161 Rat
- Unigene: 75640 Human
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Alternative names
- ANF antibody
- ANF_HUMAN antibody
- ANP antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
Immunohistochemical analysis of paraffin-embedded rat auricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Granularly cytoplasmic and perinuclear staining on rat auricle (PMID: 2942710, PMID: 1824903).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.com/ps/products/225/ab225873/Images/ab225873-324890-anti-natriuretic-peptides-a-antibody-epr20247-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)Immunohistochemical analysis of paraffin-embedded rat ventricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Weak staining on rat ventricle (PMID: 2942710, PMID: 1824903).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.com/ps/products/225/ab225873/Images/ab225873-3-ab209232279617209232IHCFr.jpg)
Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Natriuretic peptides A with ab209232 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Cytoplasmic staining on the auricula of rat heart (PMID: 2942710, PMID: 1824903).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.com/ps/products/225/ab225873/Images/ab225873-2-ab209232279616209232IP.jpg)
Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)Natriuretic peptides A was immunoprecipitated from 0.35 mg of rat heart lysate with ab209232 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab209232 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Rat heart lysate 10 µg (Input).
Lane 2: ab209232 IP in rat heart lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209232 in rat heart lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.com/ps/products/225/ab225873/Images/ab225873-286691-225873IHCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)Immunohistochemical analysis of paraffin-embedded human heart tissue labeling Natriuretic peptides A with ab209232 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Granularly cytoplasmic and perinuclear staining on human heart (PMID: 2942710, PMID: 1824903).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209232).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)](https://www.abcam.com/ps/products/225/ab225873/Images/ab225873-6-benefits-of-recombinant-antibodies.png)
Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab225873 has not yet been referenced specifically in any publications.
