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RecombinantRabMAb

Recombinant Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)
  • Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)
  • Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)
  • Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)
  • Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22089-283] to Natriuretic peptides A - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free
    See all Natriuretic peptides A primary antibodies

  • Description

    Rabbit monoclonal [EPR22089-283] to Natriuretic peptides A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse heart tissue.

  • General notes

    ab237632 is the carrier-free version of ab225844.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab237632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Notes
WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

Target

  • Function

    Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

  • Involvement in disease

    Atrial standstill 2
    Atrial fibrillation, familial, 6

  • Sequence similarities

    Belongs to the natriuretic peptide family.

  • Post-translational
    modifications

    Cleaved by CORIN upon secretion to produce the functional hormone.
    Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • ANF antibody
      • ANF_HUMAN antibody
      • ANP antibody
      • ATFB6 antibody
      • Atrial natriuretic factor antibody
      • Atrial natriuretic peptide antibody
      • Atriopeptin antibody
      • Cardiodilatin antibody
      • Cardiodilatin related peptide antibody
      • Cardiodilatin-related peptide antibody
      • Cardionatrin antibody
      • CDD ANF antibody
      • CDD antibody
      • CDD-ANF antibody
      • CDP antibody
      • Natriuretic peptide A antibody
      • Natriuretic peptide precursor A antibody
      • NPPA antibody
      • PND antibody
      • Prepronatriodilatin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)

      Immunohistochemical analysis of paraffin-embedded rat heart atrium tissue labeling Natriuretic peptides A with ab225844 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in cardiac muscle of rat atrium (PMID: 2942710; PMID:25532015; PMID: 1824903). Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225844).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)
      Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)

      Natriuretic peptides A was immunoprecipitated from 0.35 mg Mouse heart tissue lysate with ab225844 at 1/1000 dilution. Western blot was perfromed from the immunoprecipitate using ab225844 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

      Lane 1: Mouse heart lysate 10 μg (Input).
      Lane 2: ab225844 IP in Mouse heart tissue lysate (+).
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225844 in Mouse heart lysate (-).

      Blocking and dilution buffer and concentration: 5% NFDM/TBST
      Exposure time: 10 seconds

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225844).

    • Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)
      Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Natriuretic peptides A with ab225844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in cardiac muscle of rat atrium is observed.

      The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225844).

    • Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)
      Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse heart tissue labeling Natriuretic peptides A with ab225844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in cardiac muscle of mouse atrium is observed.

      The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225844).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)

      Immunohistochemical analysis of paraffin-embedded mouse heart atrium tissue labeling Natriuretic peptides A with ab225844 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in cardiac muscle of mouse atrium (PMID: 2942710; PMID:25532015; PMID: 1824903). Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225844).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)

      Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling Natriuretic peptides A with ab225844 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strong cytoplasmic staining mainly in the atria and at lower levels in ventricle of mouse heart (PMID: 2942710; PMID:25532015; PMID: 1824903). Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225844).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)
      Anti-Natriuretic peptides A antibody [EPR22089-283] - BSA and Azide free (ab237632)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab237632? Please let us know so that we can cite the reference in this datasheet.

    ab237632 has not yet been referenced specifically in any publications.

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