Overview

Description

  • Nature
    Native
  • Source
    Native

Specifications

Our Abpromise guarantee covers the use of ab742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    SDS-PAGE denaturating : multiple protein bands 150 – 210 kDa
  • Applications

    ELISA

  • Form
    Liquid
  • Additional notes
    Blood samples from tissue donors were tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV.SDS-PAGE denaturating : multiple protein bands 150 – 210 kDa
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 0.268% PBS, 2% Methyl mannoside

General Info

  • Alternative names
    • Carcinoembryonic antigen
    • Carcinoembryonic antigen-related cell adhesion molecule 5
    • CD66e
    • CEA
    • Ceacam5
    • CEAM5_HUMAN
    • DKFZp781M2392
    • Meconium antigen 100
    • OTTHUMP00000199032
    • OTTHUMP00000199033
    • OTTHUMP00000199034
    see all
  • Function
    Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
  • Tissue specificity
    Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 7 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
  • Cellular localization
    Cell membrane.
  • Information by UniProt

References

This product has been referenced in:
  • Sato M  et al. Development and validation of scFv-conjugated affinity silk protein for specific detection of carcinoembryonic antigen. Sci Rep 7:16077 (2017). Read more (PubMed: 29167497) »
  • Li Y  et al. Carcinoembryonic antigen interacts with TGF-{beta} receptor and inhibits TGF-{beta} signaling in colorectal cancers. Cancer Res 70:8159-68 (2010). Read more (PubMed: 20889724) »
See all 2 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for contacting us and your interest in our products.

The Carcino Embryonic Antigen CEA protein (ab742) is isolated from colon carcinoma liver metastatic tissue. The protein is therefore in its native form and should maintain the post-translational modifications such as glycosylation.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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Answer

Thank you for your enquiry.

I can confirm that ab4451 and ab15987 have been tested and guarantee for use in sELISA together according to the datasheets. Theab742 Carcino Embryonic Antigen CEA protein is full length native protein so should be suitable as a standard for use with this antibodies.

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your enquiry. Unfortunately, we have not determined the isoelectric point or the sequence for this product. The product is purified from colon carcinoma liver metastatic tissue and the purity is 95% by SDS-PAGE. I hope this information will still be useful. If there is anything else that I can help you with, please do not hesitate to contact me.

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Answer

Thank you for your enquiry. Products ab10036 and ab10039 work as a pair in sandwich immunoassay, but we have not tested them with ab742 antigen, so I am not able to comment about that. If you decide to go ahead and purchase the products, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. We reward each Abreview with Abpoints which can be used for discounts off future purchases and gifts. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry and patience. We unfortunately do not have any additional information with regards to the epitope specificity(which was given per ISOBM classification). NCA2 is nonspecific cross-reacting antigen 2. Ab742 is suitable as immunogen for antisera production, tracer for iodination and standard for immunoassay. We don't have any information regarding its use with ab10039 in Western blotting. If you have any additional questions, please contact us again.

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Answer

Thank you for your enquiry. For the following antibodies, epitope specificity is given per ISOBM classification and we have only following information: Ab10038 - Epitope specificity group IV, cross-reactive with NCA2. Ab10039 - Epitope specificity group IV. Ab10037 - Epitope specificity group IVa or IV/VI. Ab11330 - The epitope has not been determined; this antibody reacts specifically with human CEA (CD66e, 180 kDa) from several types of malignant tissues including colorectal, lung and breast tumors. Ab10038 is not suitable for use in Western blotting. Ab742 is suitable as immunogen for antisera production, tracer for iodination and standard for immunoassay. Specificity was determined by SDS-PAGE denaturating: multiple protein bands 150 - 210 kDa. Ab2282 is the mitogen free version of ab742. Ab2282 is suitable as an immunogen for antisera production, a tracer for iodination and as a standard for immunoassays. It is specifically depleted from traces of mitogens, therefore it is suitable for in vitro and in vivo studies of specific immune responses (vaccine trials).

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Answer

The CEA antigen is purified from a single patient source, colon carcinoma liver metastatic tissue, by immunoaffinity purification. Unfortunately we have not checked the presence of membrane anchor in the preparation.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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