Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MSDIEEVVEEYEEEEQEEAAVEEQEEAAEEDAEAEAETEETRAEEDEEEE EAKEAEDGPMEESKPKPRSFMPNLVPPKIPDGERVDFDDIHRKRMEKDLN ELQALIEAHFENRKKEEEELVSLKDRIEKRRAERAEQQRIRNEREKERQN RLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKQAQTERKSGK RQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQTIYNLEAEKFDL QEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK

Specifications

Our Abpromise guarantee covers the use of ab9937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form
    Liquid
  • Additional notes
    Native Human Cardiac Troponin-T
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 36.03% Urea, 0.117% Beta mercaptoethanol, 0.79% Tris HCl, 0.029% EDTA, 1.45% Sodium chloride

General Info

  • Alternative names
    • Cardiac muscle troponin T
    • Cardiomyopathy dilated 1D (autosomal dominant)
    • Cardiomyopathy hypertrophic 2
    • CMD1D
    • CMH2
    • CMPD2
    • cTnT
    • LVNC6
    • MGC3889
    • OTTHUMP00000033864
    • OTTHUMP00000033865
    • OTTHUMP00000033866
    • OTTHUMP00000033867
    • OTTHUMP00000033870
    • OTTHUMP00000218095
    • RCM3
    • TNNT 2
    • TNNT2
    • TNNT2_HUMAN
    • TnTc
    • Troponin T cardiac muscle
    • Troponin T type 2 (cardiac)
    • Troponin T type 2 cardiac
    • Troponin T, cardiac muscle
    • Troponin T2 cardiac
    see all
  • Function
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity
    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in disease
    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similarities
    Belongs to the troponin T family.
  • Information by UniProt

References

ab9937 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Answer

Thank you for contacting us.

Yes, I can confirm the ab105441is the biotinylated form of ab8295, and this antibody shows a good affinity towards the native protein as can be seen in the ELISA and IHC data.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you very much for your patience while I was investigating.


I am happy to confirm that we do have antibody pairs validated for sandwich ELISA for Troponin T, C and I. We do not have tested troponin proteins as standard. For the Troponin T,I have also double checked with the source of the antibodies, and they do have a protein which they can recommend. I am currently checking with my colleagues if we can add it to our catalog. However we do have the individual standard proteins or also a recomplexed protein, which could be tested also as a standard. Please let me know what you think. Indeed, it could be that the recomplexed protein is more stable and less prone to protein degradation and you could use it for all the three ELISAs. potential standard complex: ab94712 https://www.abcam.com/index.html?datasheet=94712 (or use the following: https://www.abcam.com/index.html?datasheet=94712).

Troponin C:
capture antibody: ab8285 https://www.abcam.com/index.html?datasheet=8285 (or use the following: https://www.abcam.com/index.html?datasheet=8285).
detection antibody: ab8286 https://www.abcam.com/index.html?datasheet=8286 (or use the following: https://www.abcam.com/index.html?datasheet=8286).
potential standard protein: ab68911, ab70106 or ab9932 https://www.abcam.com/index.html?datasheet=68911 (or use the following: https://www.abcam.com/index.html?datasheet=68911).

Troponin I:
capture antibody: ab115704 https://www.abcam.com/index.html?datasheet=115704 (or use the following: https://www.abcam.com/index.html?datasheet=115704).
detection antibody: ab19615 https://www.abcam.com/index.html?datasheet=19615 (or use the following: https://www.abcam.com/index.html?datasheet=19615).
potential standard protein: ab50822 https://www.abcam.com/index.html?datasheet=50822 (or use the following: https://www.abcam.com/index.html?datasheet=50822).

Troponin T:
capture antibody: ab9937 https://www.abcam.com/index.html?datasheet=9937 (or use the following: https://www.abcam.com/index.html?datasheet=9937).
detection antibody: ab19615 https://www.abcam.com/index.html?datasheet=19615 (or use the following: https://www.abcam.com/index.html?datasheet=19615).
potential standard protein: ab9937, evt new one https://www.abcam.com/index.html?datasheet=9937 (or use the following: https://www.abcam.com/index.html?datasheet=9937).

For all the proteins or for the complex, we are happy to provide you with our testing discount code as well. Please let me know whether you are interested and I am happy to send you the detailed description of this offer (you would buy the protein to the normal price, test it in ELISA, give us the feedback and then you will get another protein (or other product) for the same price for free. So basically the offer is 100% however only on a second product - for more information, please see also https://www.abcam.com/test-vial)

Unfortunately we do not have any ELISA kits for the moment against the cardiac Troponins. We are however looking into adding them to our catalog within a few months.

I hope this information is helpful. Please do not hesitate to contact me again, should you have any additional questions or should you like to receive the testing discount.

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Answer

Thank you for your email. I am sorry for having generated a confusion. I will call you tomorrow morning (I guess this will be afternoon for you).

Meanwhile please have a look at our sandwich ELISA protocol. With "work together" I ment, that one can be used as capture, and the other as detection antibody. They will not bind to each other, however they can be used as a pair as they will be able to bind the protein of interest in the same time (and not inhibit sterically each other binding).

https://www.abcam.com/index.html?pageconfig=resource&rid=11422
https://www.abcam.com/ps/pdf/protocols/Sandwich_Elisa_diagram.pdf

I look forward to talk to you tomorrow. I wish you a good evening.

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Answer

Thank you for your email.

The two antibodies will work together. The protein we do not know whether it will work in ELISA.

This is why we could give you a testing discount on the protein. This means, you would buy the protein to the normal price, test it in ELISA, give us the feedback and then you will get another protein (or other product) for the same price for free. So basically the offer is 100% however only on a second product.

The details of the offer can also be found in the last email, where I explained the offer step by step.

Please let me know if this is not clear and if you would wish to clarify some other points.

I hope this is helpful. Please do not hesitate to contact us for any additional information.

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Answer

Thank you very much for your interest in our products.

I am happy to confirm that the ab8295 and the ab8296 are perfectly well choosen together. They will work as a sandwich pair in ELISA.

The ab9937, which you would use as a standard, has not yet been tested in ELISA. It might well work, depending on its conformation. As however we have not assessed experimentally whether the ab9937 can be used in ELISA as a standard, I can offer a discount off a future purchase. If you buyab9937 now, test it inELISA as a standard for ab8295 and ab8296and submit feedback to us in the form of an Abreview, you would receive a discount worht the value of this protein. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab9937 in ELISA. I will then send a discount code. This code must be issued before purchasing ab9937 so please wait for my reply before ordering.

2. Purchase ab9937 either by phone, fax, or online (www.abcam.com).

3. Test it in ELISA.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifab9937 turns out to be unsuitable for ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer



Could you please specify for me which product your question is refering to: ab9937 or ab96516?


One of the most common compositions of PBS is as follows:




Salt Concentration Concentration
(—) (mmol/L) (g/L)
NaCl 137 8.01
KCl 2.7 0.20
Na2HPO4 - 2 H2O 10 1.78
KH2PO4 2.0 0.27
pH 7.4 7.4



Thank you and I look forward to hear back.

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Answer

For ab9937, the concentration was determined by A280, assuming an extinction coefficient of 16,960 cm-1 M-1 at 280 nm and a molecular weight of 34,575.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. I would recommend to use : 1) For the detection of Cardiac Troponin T : - ab45932 as capture antibody (directed against amino acids 64-82) www.abcam.com/ab45932 , - ab9937 as protein target, www.abcam.com/ab9937 , (not ab47005 which is the immunogen peptide used to raise ab45932), - ab105439 as detection antibody (directed against amino acids 64-82), www.abcam.com/ab105439 . 2) For the detection of Cardiac Troponin I : - ab115704 as capture antibody, www.abcam.com/ab115704 , - ab50803 as protein target, www.abcam.com/ab50803 , - ab19615 as detection antibody (can be conjugated with FITC using the EasyLink conjugation kit), www.abcam.com/ab19615 . I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for you enquiry. Unfortunately we do not have details of the charge of cardiac Troponin T protein (ab9937) on our data sheet. However, I have done some literature searching and according to Pearlstone et al., (1986) (see the link below) the protein is negatively charged at neutral pH. http://www.jbc.org/cgi/content/abstract/261/36/16795 Please do not hesitate to contact me should you require further assistance.

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