Natural human Cathepsin D protein (ab91123)



  • Nature
  • Source
  • Amino Acid Sequence
    • Species


Our Abpromise guarantee covers the use of ab91123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Activity: >=300 units per mg protein. One unit is defined as the amount of enzyme that digests hemoglobin-releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A280 of 1.0 per 60 minutes at 37°C . Substrate: acid denatured hemoglobin, pH 1.8 (0.2% in reaction mixture). Buffer: 100 mM formate, pH 3.3.
  • Applications

    Functional Studies


    Western blot

  • Purity
    > 95 % SDS-PAGE.

  • Form
  • Additional notes
    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.0 Prepared from tissue shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C.

    pH: 6.50
    Constituent: 0.0328% Sodium phosphate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Resuspend a 25ug vial with 71.4uL of deionized H2O to give a protein concentration of 0.350mg/mL, which is the original protein concentration of the lot when it was finished. This should then be aliquotted into suitable amounts in a screw top vial to prevent sublimation and stored at <70 deg C. Aliquots should be diluted to appropriate concentration once thawed. We recommend 2mM NaP pH 6.5 to make dilutions.

General Info

  • Alternative names
    • CatD
    • Cathepsin D
    • Cathepsin D heavy chain
    • CD
    • Ceroid lipofuscinosis neuronal 10
    • CLN10
    • CPSD
    • ctsd
    • Epididymis secretory sperm binding protein Li 130P
    • HEL S 130P
    • Lysosomal aspartyl peptidase
    • Lysosomal aspartyl protease
    • MGC2311
    see all
  • Function
    Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • Tissue specificity
    Expressed in the aorta extrcellular space (at protein level).
  • Involvement in disease
    Ceroid lipofuscinosis, neuronal, 10
  • Sequence similarities
    Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • Post-translational
    N- and O-glycosylated.
  • Cellular localization
    Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt


  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    Lane 1. Molecular weight markers
    Lane 2. 4 μg Cathepsin D (non-reduced/no heat)
    Lane 3. 7 μg Cathepsin D (non-reduced/no heat)
    Lane 4. Molecular weight markers
    Lane 5. 4 μg Cathepsin D (reduced/heated)
    Lane 6. 7 μg Cathepsin D (reduced/heated)


ab91123 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

One unit of activity is defined as the amount of enzyme that digests hemoglobin-releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A 280 of 1.0 per 60 minutes at 37°C . Substrate: acid denatured hemoglobin, p...

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Thank you for kindly confirming these details. I am sorry this vial ofprotein has not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab66587 has been added to your...

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Thank you for your reply and for kindly confirming these details.

I have discussed this case with the originator of this protein. I can confirm there are no reports of any problems with this lot. They have also confirmed that this antibody i...

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Thank you for taking the time tocontact us. I am sorry to hear the customer has some concerns regarding this protein.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our record...

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Thank you for your enquiry.

I can confirm that the reconstitution of ab91123Cathepsin D protein (Active)is lot dependent. I have reviewed your orders, and have found order number #####, and the lot number from this order is GR35865-3. Could ...

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