Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab7535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Immunoprecipitation

    ELISA

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 4.50
    Constituent: 3% Acetic acid

General Info

  • Alternative names
    • Alpha 1 type III collagen
    • Alpha1 (III) collagen
    • CO3A1_HUMAN
    • COL 3A1
    • COL3A1
    • Collagen alpha 1(III) chain
    • Collagen alpha-1(III) chain
    • Collagen III alpha 1 chain precursor
    • Collagen III alpha 1 polypeptide
    • Collagen type III alpha
    • Collagen type III alpha 1
    • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant)
    • Collagen type III alpha 1 chain
    • Collagen, fetal
    • EDS4A
    • Ehlers Danlos syndrome type IV, autosomal dominant
    • Fetal collagen
    • Type III collagen
    see all
  • Function
    Collagen type III occurs in most soft connective tissues along with type I collagen.
  • Involvement in disease
    Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
    Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
    Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
  • Sequence similarities
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

Images

  • All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution

    Lane 1 : plus ab7535 reduced and denatured
    Lane 2 : plus ab7535 native

    Secondary
    All lanes : Goat anti-mouse-HRP at 1/2000 dilution


    The protein can be used reduced + denaturated (band size ~130 kDa) or native (two bands ~ 130kDa and ~300kDa) The native protein gives a brighter signal.

    PH of the protein lysate is low - a loading dye with bromphenolblue changes its colour. This does not effect the western blot.

    See Abreview

References

This product has been referenced in:
  • Di Francesco S  et al. Red grape (Vitis vinifera L.) flavonoids down-regulate collagen type III expression after UV-A in primary human dermal blood endothelial cells. Exp Dermatol 27:973-980 (2018). Read more (PubMed: 29742305) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abreviews
Application
ELISA
Capture antibody Anti collagen III antibody ab6310

Natural human protein Natural human collagen III protein ab7535 https://www.abcam.com/natural-human-collagen-iii-protein-ab7535.html

Detection antibody Anti collagen III antibody biotin ab6580

Abcam user community

Verified customer

Submitted Jun 07 2017

Application
SDS-PAGE
Loading amount: 250 ng collagen
Gel: Reduced denaturing (7% Tris acetate gel (Novex))
Lane 1 standards
Lane 2 blank
Lane 3 MMP-13
Lane 4 ab7533
Lane 5 ab7533 + MMP-13 (62 ng)
Lane 6 ab7533 + MMP-13 (31 ng)

Abcam user community

Verified customer

Submitted Nov 18 2015

Answer

Thank you for contacting us.

I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Question
Answer

I realized that I may not have written down the ID number of the product you were interested in correctly. Could you please send me the correct abID number and I will be able to complete you request.

Read More
Application
Other

Review text: The protein can be used reduced + denaturated (band size ~130 kDa)
or native (two bands ~ 130kDa and ~300kDa)
The native protein gives a brighter signal.

PH of the proteinlysate is low - a loading dye with bromphenolblue changes its colour. This does not effect the western blot.
Sample: Human Purified protein

Primary antibody (in addition to 'Collagen III protein')
Primary antibody: Abcam primary antibody: Anti-Collagen III antibody [FH-7A] (ab6310)
Dilution: 1/500

Secondary antibody
Name: Non-Abcam antibody was used: goat anti mouse IgG-HRP, Santa Cruz-2005, lot c100
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/2000

Ms. Michaela Leyh

Verified customer

Submitted Feb 09 2011

Question
Answer

These products have been prepared from human placenta and are chromatographically and immunologically pure. They are free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. Reaction with our anti-Collagen antibodies I, II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA) apart from the expect cross reaction with the collagen type i.e ab7535 cross reacts with the anti-collagen III antibody, and ab7533 cross reacts with the anti-collagen I antibody.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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