Native Human Collagen VI protein (ab7538)
Key features and details
- Expression system: Native
- Suitable for: SDS-PAGE, WB, IP, ELISA, IHC-P
Description
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Product name
Native Human Collagen VI protein
See all Collagen VI proteins and peptides -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Additional sequence information
Prepared from Human Placenta.
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Specifications
Our Abpromise guarantee covers the use of ab7538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
Immunoprecipitation
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Form
Liquid -
Additional notes
This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type VI. Reaction with anti-Collagen I, II, III, IV or V is negligible (typically less than 1% cross reactivity was detected by ELISA).
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C. Diluted stock solutions should be used immediately and not be frozen. Please see notes section. Store undiluted.
Preservative: 0.01% Sodium azide
Constituent: 3% Acetic acid
General Info
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Alternative names
- Alpha 1 (VI) chain (61 AA)
- CO6A1_HUMAN
- COL6A1
see all -
Function
Collagen VI acts as a cell-binding protein. -
Involvement in disease
Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy. -
Sequence similarities
Belongs to the type VI collagen family.
Contains 3 VWFA domains. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab7538 has been referenced in 4 publications.
- Holm Nielsen S et al. A Fragment of Collagen Type VI alpha-3 chain is Elevated in Serum from Patients with Gastrointestinal Disorders. Sci Rep 10:5910 (2020). PubMed: 32245981
- Wishart AL et al. Decellularized extracellular matrix scaffolds identify full-length collagen VI as a driver of breast cancer cell invasion in obesity and metastasis. Sci Adv 6:N/A (2020). PubMed: 33087348
- Spiers RM et al. Donor age significantly influences the Raman spectroscopic biomolecular fingerprint of human pancreatic extracellular matrix proteins following collagenase-based digestion. Acta Biomater 99:269-283 (2019). PubMed: 31525537
- Veidal SS et al. MMP Mediated Degradation of Type VI Collagen Is Highly Associated with Liver Fibrosis - Identification and Validation of a Novel Biochemical Marker Assay. PLoS One 6:e24753 (2011). PubMed: 21935455