Native Human Factor Va protein (ab62415)
Key features and details
- Expression system: Native
- Purity: > 95% Affinity purified
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Native Human Factor Va protein -
Purity
> 95 % Affinity purified.
Purity is determined by SDS-PAGE analysis -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human
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Specifications
Our Abpromise guarantee covers the use of ab62415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
Concentartion varies from lot to lot but is between 5-10mg/ml -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 0.0555% Calcium chloride, 50% Glycerol
General Info
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Alternative names
- Activated protein c cofactor
- Coagulation factor V
- Coagulation factor V (proaccelerin, labile factor)
see all -
Relevance
Factor Va is a cofactor for the serine protease factor Xa, and in the presence of calcium ions they collectively assemble on a phospholipid surface to form the prothrombinase complex. The prothrombinase complex is responsible for the rapid conversion of prothrombin to thrombin. Factor Va is derived from the pro-cofactor, factor V, upon limited proteolysis by alpha-thrombin. The thrombin cleavage of factor V liberates two heavily glycosylated activation peptides from the central portion of the molecule which have no cofactor function. Factor Va is comprised of an NH2-terminal derived heavy chain (MW 94kDa) and a COOH-terminal derived light chain (MW 74kDa) which remain associated in the presence of calcium ions. The cofactor binds to phospholipid (cell membrane) surfaces and effectively serves as a receptor for membrane bound factor Xa. Complete assembly of the prothrombinase complex (factor Xa, factor Va, phospholipid, and calcium) results in a 300,000-fold increase in the rate of prothrombin conversion relative to the rate observed with factor Xa alone. The interaction between factor Va and factor Xa is mediated by both the heavy and light chain of factor Va, while the binding of prothrombin to factor Va is mediated solely by the heavy chain. -
Cellular localization
Cell Membrane and Plasma membrane
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab62415 has not yet been referenced specifically in any publications.