Overview

  • Product name
    Natural human FSH protein
  • Protein length
    Full length protein

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Human
    • Molecular weight
      36 kDa
    • Additional sequence information
      hFSH is a glycoprotein produced from urine of post-menopausal women and having a total MW of 35.5 kDa. hFSH is a heterodimeric hormone consisting of 165 aa chain (non-active)& 115 aa chain (active).

Specifications

Our Abpromise guarantee covers the use of ab51888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    This protein is biologically active when compared to standard. The activity was found to be 626IU/mg. Contaminants are less than:- 0.1% HCG 0.5% TSH 0.5% LH 0.5% GH 0.5% Prolactin. Free of HbsAg, antibodies to HIV and HCV.
  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    98% as determined by SDS-PAGE and HPLC. Purified using proprietary chromatographic techniques from urine of post menopausal women.
  • Form
    Lyophilised
  • Additional notes
    For long term storage it is recommended to add a carrier protein such as 0.1% BSA or HSA.


    This protein, Human FSH is a glycoprotein produced from urine of post-menopausal women and having a total molecular mass of 35,500 Dalton. hFSH is a heterodimeric hormone consisting of 165 amino acids chain (non-active) and 115 amino acids chain (active).

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in sterile distilled water to give a concentration of 0.1-1.0 mg/ml.

General Info

  • Alternative names
    • Anterior pituitary glycoprotein hormones common subunit alpha
    • CG alpha
    • CGA
    • Choriogonadotropin alpha chain
    • Chorionic gonadotrophin alpha subunit
    • Chorionic gonadotropin alpha polypeptide
    • Follicle stimulating hormone alpha chain
    • Follicle Stimulating Hormone Beta
    • Follicle stimulating hormone beta polypeptide
    • Follicle stimulating hormone beta subunit
    • Follitropin alpha chain
    • Follitropin beta chain
    • Follitropin subunit beta
    • FSH A
    • FSH alpha
    • FSH B
    • FSH beta
    • FSHA
    • FSHB
    • Glycoprotein hormones alpha chain
    • Glycoprotein hormones alpha polypeptide
    • GPHa
    • GPHA 1
    • GPHA1
    • HCG
    • LHA
    • LSH alpha
    • Luteinizing hormone alpha chain
    • Lutropin alpha chain
    • OTTHUMP00000016825
    • Thyroid stimulating hormone alpha chain
    • Thyrotropin alpha chain
    • TSH alpha
    • TSHA
    see all
  • Relevance
    Follicle stimulating hormone enables ovarian folliculogenesis to the antral follicle stage and is essential for Sertoli cell proliferation and maintenance of sperm quality in the testis. Members of the pituitary glycoprotein hormone family, of which FSH is one (see also luteinizing hormone, chorionic gonadotropin, and thyroid stimulating hormone), consist of a shared alpha chain and a beta chain encoded by a separate gene. The FSHB gene encodes the beta subunit of follicle stimulating hormone.
  • Cellular localization
    Secreted

References

ab51888 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your inquiry.

The activity for this lot is also 6261 U/mg.

Please let me know if you have any other questions.

Read More

Answer

Thank you for your phone call. I am sending you the following discount codes for trying the antibodies in sELISA and mouse/rat. As for the proteins, I have found the following:

Luteinizing Hormone: none
FSH: ab51888 (human) - test in sELISA
TSH: ab110682 (human) - test in sELISA

Since the FSH and TSH proteins have not yet been tested in sELISA, I am sending you also discount codes for these proteins (see below). Unfortunately, we do not carry the Luteinizing Hormone protein.

In terms of secondary antibodies, you would only need one for the Luteinizing Hormone sELISA as none of these antibodies has an HRP label. The other antibody pairs for TSH and FSH contain an HRP-labeled primary antibody.

The suggested secondary antibodies are:
ab98799 - donkey anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98799.html)
ab98808 - goat anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98808.html)

Testing Discount Codes:

DISCOUNT CODE: xxx- test in mouse, rat
DISCOUNT CODE: xxx- test in mouse, rat
DISCOUNT CODE: xxx- test in sELISA, mouse, rat
DISCOUNT CODE: xxx-test in sELISA, mouse, rat
DISCOUNT CODE: xxx- test in mouse, rat
DISCOUNT CODE: xxx- test in mouse, rat

DISCOUNT CODE: xxx ab51888 (human FSH protein) - test in sELISA
DISCOUNT CODE: xxx ab110682 (human TSH protein) - test in sELISA

Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test thes antibodies and proteins in sELISA and nouse/rat. Each code will give you 1 free PRIMARY ANTIBODY or PROTEIN before the expiration date. To redeem this offer, please submit each an Abreview for sELISA and mouse/rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Please also remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Please let me know if there is anything else I can help you with.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up