Native Human Haptoglobin protein (ab90924)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
Description
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Product name
Native Human Haptoglobin protein
See all Haptoglobin proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Sequence
MSALGAVIALLLWGQLFAVDSGNDVTDIADDGCPKPPEIAHGYVEHSVRY QCKNYYKLRT EGDGVYTLNDKKQWINKAVGDKLPECEADDGCPKPPEI AHGYVEHSVRYQCKNYYKLRTE GDGVYTLNNEKQWINKAVGDKLPECE AVCGKPKNPANPVQRILGGHLDAKGSFPWQAKMV SHHNLTTGATLINE QWLLTTAKNLFLNHSENATAKDIAPTLTLYVGKKQLVEIEKVVLHP NY SQVDIGLIKLKQKVSVNERVMPICLPSKDYAEVGRVGYVSGWGRNANFKF TDHLKYVM LPVADQDQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFC AGMSKYQEDTCYGDAGSAFA VHDLEEDTWYATGILSFDKSCAVAEYGV YVKVTSIQDWVQKTIAEN
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab90924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Additional notes
Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.20. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20ºC.
Constituent: 100% Haptoglobin
General Info
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Alternative names
- Binding peptide
- BP
- Haptoglobin alpha chain
see all -
Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Anhaptoglobinemia -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE analysis of ab90924: 4-12% Bis-Tris-HCl NuPAGE gel
Lane 1: Haptoglobin - 5 μg (reduced/ heated) Lane 2: Haptoglobin - 10 μg (reduced/ heated)
Lane 3: Haptoglobin - 20 μg (reduced/ heated) Lane 4: Molecular weight markers
Lane 5: Haptoglobin - 5 μg (non-reduced/ no heat) Lane 6: Haptoglobin - 10 μg (non-reduced/ no heat)
Lane 7: Haptoglobin - 20 μg (non-reduced/ no heat)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab90924 has been referenced in 2 publications.
- Moothanchery M et al. Rapid and sensitive detection of ovarian cancer biomarker using a portable single peak Raman detection method. Sci Rep 12:12459 (2022). PubMed: 35864143
- Perumal J et al. SERS-based detection of haptoglobin in ovarian cyst fluid as a point-of-care diagnostic assay for epithelial ovarian cancer. Cancer Manag Res 11:1115-1124 (2019). PubMed: 30774440