The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Activity was measured by clotting and/or chromogenic substrate assay, following conversion of prothrombin to thrombin.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 50% dH2O, 50% ddH20, 50% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Prethrombin 2, the smallest single-chain immediate precursor of a-thrombin (corresponding to residues Thr272 to Glu579 in prothrombin), has one glycosylation site at Asn373 and four disulfide bonds, Cys293-Cys439, Cys348-Cys364, Cys493-Cys507, and Cys521-Cys551.
alpha-Thrombin can be derived from prothrombin by two pathways, each of which generates different intermediate products. In one pathway, human prothrombin is converted relatively slowly to alpha-thrombin in the presence of factor Xa and Ca2+ ions by initial cleavage at residue (Arg) 271/(Thr) 272 with consequent production of fragment 1.2 and prethrombin 2. Prethrombin 2 is then cleaved at R 320 with consequent generation of alpha-thrombin. In the other pathway, initial cleavage at R 320 yields an intermediate product, meizothrombin.
Secreted, extra cellular space
has not yet been referenced specifically in any publications.
Publishing research using ab62535? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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