Anti-Natural Killer Cell Activation Structures antibody [ANK61] (ab36392)

Overview

  • Product name
    Anti-Natural Killer Cell Activation Structures antibody [ANK61]
  • Description
    Mouse monoclonal [ANK61] to Natural Killer Cell Activation Structures
  • Host species
    Mouse
  • Specificity
    The antigen recognized by ab36392 is highly expressed on freshly isolated and cultured NK cells. The antibody also binds to rat alpha beta TCR T cells and at a low level to B cells.
  • Tested applications
    Suitable for: Flow Cyt, IP, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Cell preparation of IL 2 activated cultured NK cells (Wag rats)

  • General notes


    Triggering of the antigen by ab36392 antibody activates the lytic machinery of rat NK cells, but not of T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    Triggering of the antigen by ab36392 antibody activates the lytic machinery of rat NK cells, but not of T cells.
  • Clonality
    Monoclonal
  • Clone number
    ANK61
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Use at an assay dependent concentration.

IHC-P Use at an assay dependent concentration.

Use at an assay dependent concentration.

Target

  • Relevance
    Natural killer cells (NK) are a type of lymphocyte and a component of innate immune defense. They share a common progenitor with T cells and have been described as large, granular, bone-marrow derived lymphocytes. These cells do not destroy the attacking microorganisms directly; they attack infected cells and cells that appear that they might be cancer. NK cells are not phagocytic; they weaken the target cell's plasma membrane, causing water and ions to diffuse into the cell and expanding it. Under this large pressure, the target cell lyses. NK cells are characterised immunohistochemically by the presence of CD56 and the absence of CD3 on the cell membrane. NK cells are activated in response to interferons or macrophage-derived cytokines. They serve to contain virus infections while the adaptive immune response is generating antigen specific cytotoxic T cells that can clear the infection. Patients deficient in NK cells prove to be highly susceptible to early phases of herpes virus infection.

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your email.

There may be some antibodies where I might have suggested the human tissues or cells. I apologize for this. I have included the positive control information irrespective of species so similar mouse tissues sections can used as positive control.

Actually the positive control information for each antibody is present on the datasheet; please check the datasheet. Alternatively, we always follow human protein Atlas database (http://www.proteinatlas.org/index.php). The second option is, searching positive control for other antibodies in catalogue against same target; the cell line or tissue sections tested with those antibodies could also be used as positive control. The third place to search for is literature.

I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

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Answer

Thank you for contacting us.

The positive control for ab36392 is IL2 activatedcultured NK cells.

ab9918; human tonsil sections, human cervical squamous cell carcinoma section

ab34674; human artery or colon tissue sections

ab15537; HepG2 cells. mouse brain sections, human placenta sections; Human hepatocellular liver carcinoma cell line; Human hepatocarcinoma tissue sections

ab2557; mouse spleen tissue sections

ab9475; human tonsil tissue sections

ab22378; mouse spleen tissue sections

ab51312; Thymus tissue sections, human pancreas tissue sections.

The best database to search for positive control is Human Protein Atlas. Please click the link http://www.proteinatlas.org/index.php

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your reply.

It does look like we changed the storage instructions of ab2557. Since this product was tested in a guaranteed species and technique and she wasn't seeing any staining, we could offer a free of charge replacement order from the original order. Please let me know if that is what the customer would prefer.

For the spotty staining in IHC, it may be that the antibody is binding to the blocking buffer. She should filter the blocking buffer before adding to the slide. Also she should run a no primary control to determine if the secondary is binding to something non-specifically in the slide. Also the tissue maynot washed enough orfixative may still present. And though they used a gentle permeabilization agent, pemeabilization may have damaged the membrane and removed the membrane protein
Remove permeabilizing agent from your buffers.

I hope this information helps. Please let me know what the customer would like to do with the ab2557.

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Answer

Thank you forhaving your customercomplete our questionnaire. I am sorry to hear that these antibodies are not providing satisfactory results for them. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

There are some issues with the antibodies that may be resulting in the lack of staining. Firstly, the neutrophil antibody ab2557 was stored in the refrigerator. The datasheet for this antibody says to store at -20C. The last order I have from you for this product is in January. It is possible that storing this antibody improperly for 4 months has affected its quality.

Secondly, the NK antibody ab36392 is not tested in mouse, only rat. We do not guarantee this antibody to work in mouse. Does the customer have access to rat tissue to run a positive control? Also this is the antibody that was giving spotty staining. Have they run a no-primary control? Or tried filtering the blocking buffer?

Lastly, the interferon gamma antibody ab9918 is not tested in IHC-P or ICC and is therefore not guaranteed to work in these applications.

Since two of the antibodies are being used in untested ways and the neutrophil antibody was improperly stored, there are many reasons why the customer may not be seeing staining.

I hope this information is helpful, and I thank you for your cooperation.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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