Key features and details
- Mouse monoclonal [ANK44] to Natural Killer Cell
- Suitable for: IHC-Fr, IP, Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Product nameAnti-Natural Killer Cell antibody [ANK44]
DescriptionMouse monoclonal [ANK44] to Natural Killer Cell
Specificityab36388 also binds to rat gamma-delta-TCR T cells. It does not bind to alpha-beta-TCR T cells or to B cells. The antigen recognized by ab36388 is highly expressed on rat NK cells after IL-2-activation. The antigen is not expressed by unstimulated NK cells.
Tested applicationsSuitable for: IHC-Fr, IP, Flow Cytmore details
Species reactivityReacts with: Rat
Preparation of IL-2-activated cultured NK cells (Wag rats)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.05% Sodium azide
Constituent: Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab36388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceNatural killer cells (NK) are a type of lymphocyte and a component of innate immune defense. They share a common progenitor with T cells and have been described as large, granular, bone-marrow derived lymphocytes. These cells do not destroy the attacking microorganisms directly; they attack infected cells and cells that appear that they might be cancer. NK cells are not phagocytic; they weaken the target cell's plasma membrane, causing water and ions to diffuse into the cell and expanding it. Under this large pressure, the target cell lyses. NK cells are characterised immunohistochemically by the presence of CD56 and the absence of CD3 on the cell membrane. NK cells are activated in response to interferons or macrophage-derived cytokines. They serve to contain virus infections while the adaptive immune response is generating antigen specific cytotoxic T cells that can clear the infection. Patients deficient in NK cells prove to be highly susceptible to early phases of herpes virus infection.
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ab36388 has been referenced in 5 publications.
- Travis OK et al. Interleukin-17 signaling mediates cytolytic natural killer cell activation in response to placental ischemia. Am J Physiol Regul Integr Comp Physiol 318:R1036-R1046 (2020). PubMed: 32320265
- Travis OK et al. Chronic infusion of interleukin-17 promotes hypertension, activation of cytolytic natural killer cells, and vascular dysfunction in pregnant rats. Physiol Rep 7:e14038 (2019). PubMed: 30963715
- Sun Y et al. Natural killer cells inhibit metastasis of ovarian carcinoma cells and show therapeutic effects in a murine model of ovarian cancer. Exp Ther Med 16:1071-1078 (2018). PubMed: 30116358
- Shields CA et al. Placental ischemia-stimulated T-helper 17 cells induce preeclampsia-associated cytolytic natural killer cells during pregnancy. Am J Physiol Regul Integr Comp Physiol 315:R336-R343 (2018). PubMed: 29718698
- Giezeman-Smits KM et al. Novel monoclonal antibodies against membrane structures that are preferentially expressed on IL-2-activated rat NK cells. J Leukoc Biol 63:209-15 (1998). PubMed: 9468279