Overview

  • Product name

    Anti-Natural Killer Cell antibody [ANK44]
  • Description

    Mouse monoclonal [ANK44] to Natural Killer Cell
  • Host species

    Mouse
  • Specificity

    ab36388 also binds to rat gamma-delta-TCR T cells. It does not bind to alpha-beta-TCR T cells or to B cells. The antigen recognized by ab36388 is highly expressed on rat NK cells after IL-2-activation. The antigen is not expressed by unstimulated NK cells.
  • Tested applications

    Suitable for: IHC-Fr, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Preparation of IL-2-activated cultured NK cells (Wag rats)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    ANK44
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    Natural killer cells (NK) are a type of lymphocyte and a component of innate immune defense. They share a common progenitor with T cells and have been described as large, granular, bone-marrow derived lymphocytes. These cells do not destroy the attacking microorganisms directly; they attack infected cells and cells that appear that they might be cancer. NK cells are not phagocytic; they weaken the target cell's plasma membrane, causing water and ions to diffuse into the cell and expanding it. Under this large pressure, the target cell lyses. NK cells are characterised immunohistochemically by the presence of CD56 and the absence of CD3 on the cell membrane. NK cells are activated in response to interferons or macrophage-derived cytokines. They serve to contain virus infections while the adaptive immune response is generating antigen specific cytotoxic T cells that can clear the infection. Patients deficient in NK cells prove to be highly susceptible to early phases of herpes virus infection.
  • Alternative names

    • Large granular lymphocytes antibody
    • LGLs antibody
    • NK cells antibody

References

This product has been referenced in:

  • Travis OK  et al. Chronic infusion of interleukin-17 promotes hypertension, activation of cytolytic natural killer cells, and vascular dysfunction in pregnant rats. Physiol Rep 7:e14038 (2019). Read more (PubMed: 30963715) »
  • Sun Y  et al. Natural killer cells inhibit metastasis of ovarian carcinoma cells and show therapeutic effects in a murine model of ovarian cancer. Exp Ther Med 16:1071-1078 (2018). Read more (PubMed: 30116358) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Following up my previous message, I found the original article describing the production and characterization of the natural killer cell antibody ab36388, clone ANK44. The paper suggests that the protein this antibody recognizes, or at least the epitope, is present on NK cells only after activation with IL-2. The full paper is at this link: http://www.jleukbio.org/content/63/2/209.full.pdf All their tests were done on cells, not tissue sections. The cells were fixed with acetone, so the antibody is capable of recognizing the epitope after fixation with acetone, so I do not think your choice of fixative is the problem in this case. The number of NK cells in the rat kidney expressing the target of this antibody, if the tissue is not inflamed, may be very low. Regarding the IFN gamma antibody ab7740, I have asked our source of this antibody how it was validated for IHC on frozen tissue and will send this information to you when I receive it, I expect sometime on Wednesday. Antigen retrieval should not be necessary if your tissues are fixed only with acetone. If you have tissue that has been fixed with paraformaldehyde or formalin, then an antigen retrieval step may be necessary. Please let me know if you need more advice about AR.

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