Native Staphylococcus aureus Protein A (FITC) (ab7455)
Key features and details
- Expression system: Native
- Purity: > 95% Protein A purified
- Suitable for: Flow Cyt, Immunomicroscopy
Description
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Product name
Native Staphylococcus aureus Protein A (FITC)
See all Protein A proteins and peptides -
Purity
> 95 % Protein A purified. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Staphylococcus aureus -
Predicted molecular weight
56 kDa
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Specifications
Our Abpromise guarantee covers the use of ab7455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Flow Cytometry
Immunomicroscopy
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Form
Liquid -
Additional notes
Molecular Weight (protein A): 42,000 daltons
Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, BSA
General Info
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Alternative names
- gG-binding protein A
- Immunoglobulin G binding protein A
- SPA
- Staphylococcal protein A
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Relevance
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
Cellular localization
Secreted; cell wall.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab7455 has been referenced in 1 publication.
- He B et al. Group A Rotaviruses in Chinese Bats: Genetic Composition, Serology, and Evidence for Bat-to-Human Transmission and Reassortment. J Virol 91:N/A (2017). IF . PubMed: 28381569