Immunohistochemistry (IHC)
Product
Immunohistochemistry (IHC) is an immunostaining technique widely used in research and diagnostics to study the pathophysiology of a multitude of diseases.
IHC involves staining of tissues slides, paraffin-embedded (FFPE) or frozen, with a primary antibody to study the tissue distribution of a protein of interest. Primary antibodies specifically bound the antigen of interest are detected with a secondary antibody either via chromogenic detection or fluorescent detection. Multiple antibodies can be used to simultaneously stain the same tissue slide (multiplex IHC) in order to study tissue architecture and spatial organization in healthy and pathological states.
Abcam offers an unrivaled range of recombinant, IHC-validated antibodies and IHC antibody panels for key immuno-oncology targets. We also offer multiple immunohistochemistry (IHC) kits, reagents, and accessories, which are designed to help you obtain sensitive and reproducible results.
Find our most popular IHC products:
Substrate kits: DAB | enhanced DAB | AEC
DAB IHC kits: Mouse and Rabbit | Rabbit | Mouse
Antigen retrieval buffers: Tris-EDTA pH 9.0| EDTA pH 8.0 | Citrate
Mounting media: with DAPI | without DAPI
Reagents: Streptavidin HRP | Hematoxylin solution
- All
- Articles
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- Protocols
- Video
Abreviews our review and reward program
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Troubleshooting and using controls in IHC
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Immunostaining in IHC
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Introduction to immunohistochemistry (IHC)
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Substrates and chromogens for IHC
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Multiplex imaging application note
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Antibody panels for immune checkpoints
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Powering multiplex imaging
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Multiplex tissue imaging: current methods
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Mouse on Mouse (MOM) staining protocol
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Protocols and troubleshooting tips, to help get the best from our antibodies
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IHC tissue processing protocol
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IHC/ICC Staining Techniques Using Single and Multiple Labels
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IHC staining protocol for whole mount samples
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ICC/IF protocol
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DAB staining principles, kits, and protocols
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IHC staining protocol for paraffin, frozen, and free floating sections
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IHC & ICC fixation protocol
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Sectioning of paraffin-embedded tissue video protocol
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Bielschowsky’s silver stain protocol
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IHC FAQs
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In situ hybridization protocol
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IHC antigen retrieval protocol
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IHC for paraffin-embedded sections video protocol
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IHC deparaffinization protocol
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Cancer biomarkers guide
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Detect low-abundance proteins with biotinylated antibodies
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BrdU staining protocol
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Mounting media for IHC and ISH
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Immunohistochemistry (IHC) kits and reagents
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Mouse on Mouse Polymer IHC Kit (ab269452)
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Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
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Rabbit specific HRP/DAB (ABC) Detection IHC Kit (ab64261)
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PAP pen (ab2601)
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Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab93678)
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Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681)
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Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit (ab64264)
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DAB Substrate Kit (ab64238)
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Trichrome Stain Kit (Connective Tissue Stain) (ab150686)
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