Multiplexing assays

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We offer a wide range of multiplex assays, including antibody arrays and FirePlex^® – a new particle-based multiplexed assay technology platform capable of analyzing both proteins and miRNAs.

Multiplex assays not only allow researchers to measure multiple biomarkers simultaneously but also offer the unique advantage of harvesting more actionable data to inform better decision making, accelerate research, and increase the probability of making a discovery.

FirePlex multiplex assays

FirePlex multiplex assays use Abcam’s proprietary FirePlex hydrogel particle technology for multiplexing biomolecules. The hydrogels leverage the flexibility of particle arrays while allowing the immobilization of probes within the hydrated, 3D binding environment. The optical liquid stamping of hydrogel particles enables bar-coding and multiplexing biomolecules such as miRNA and proteins. The FirePlex particles allow building assays with high multiplexibility, sensitivity, and high throughput capacity (in 384-well and 1536-well plate formats). The optimal thermodynamics of binding kinetics with FirePlex particles together with their flexibility renders the FirePlex Multiplex assay compatible for use with flow cytometers and high content imagers, eliminating the need for a dedicated instrument for assay readout.

FirePlex multiplex assays allow you to avoid the hardships encountered with traditional multiplex assays: long, tedious man-hours, large sample volume requirements, automation challenges, and lack of customization. To cover the range of popular and upcoming biomolecules for biomarkers, Abcam offers two options of the multiplex assay: the miRNA assay and the immunoassay.

Antibody arrays

Our Antibody Arrays, similar to ELISA, but with enhanced multiplexing capabilities, can analyze hundreds of different proteins simultaneously and with significantly lower cost, time, and effort.

Quantitative and semi-quantitative arrays offer 40 and 96 analyte multiplexing respectively, for comparative analysis of cytokines, chemokines, growth factors, and many other biological proteins and pathways in human, mouse, rat, and other species.

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Features

FirePlex miRNA assays

- No sample preprocessing or RNA extraction
- Small sample volumes
- Multiplexibility - up to 70-plex
- Assay readout on Flow Cytometer
- Free data analysis software

FirePlex immunoassays

- Ideal for high throughput with options of 96-well, 384-well, or 1536-well plate format
- Reduced assay prep time with no-wash protocols for 384 and 1536-well plate format
- Automation friendly
- Assay readout on a flow cytometer or high content imager
- Free data analysis software

High-throughput FirePlex assays

A new high-throughput, automation-friendly immunoassay format to facilitate high-volume biomarker testing throughout the drug discovery pipeline. With 384- and 1536-well plate format, streamlined workflow, and an easy readout on high-content imagers, FirePlex high-throughput assays will accelerate your path to clinical success.

Antibody arrays

Cytokine arrays and other antibody arrays can be used to measure the levels of hundreds of cytokines in a sample at the same time. Analyze just like a chemiluminescent western blot or with a microarray slide scanner.