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Abcam's proprietary FirePlex particle technology is ideal for multiplexing protein targets. The bar-coded hydrogel particles allow the bulk immobilization of capture antibody, providing enhanced binding capacity in comparison to surface-functionalization techniques. The flexibility of the hydrogel matrix reduces steric constraints on the binding while enhancing binding kinetics in a planar array format, making assay readouts possible both on flow cytometers as well as high content imagers.
We offer FirePlex immunoassays in three main formats:
FirePlex-96 assay: ideal for simple, straightforward workflows in biomarker profiling
- Up to 70-plex in 96-well plate
FirePlex-384 assay: ideal for high-throughput screening
- Up to 10-plex high-throughput in 384-well plate format
- No-wash, two-step - automation friendly
FirePlex-1536 assay: ideal for high-throughput screening
- Up to 10-plex high-throughput in 1536-well plate format
- No-wash, one-step - automation recommended
FirePlex Assay | Instrumentation | Automation | Sample Input* | Hands-on time | Dynamic Range | Sensitivity | |
Required | Optional | ||||||
Flow Cytometer; Vacuum plate washer | Liquid handler | Optional | 12.5 µl | 2.5 hours | 5 logs | sub pg - pg | |
High Content imager | Liquid handler | Optional | 6.25 µl | 30 min | 3-4 logs | pg | |
High Content imager | Liquid handler | Recommended | 2.5 µl | 1 hour | 3 logs | pg | |
*Volumes listed for 1:2 dilution.
Need something more specific?
Mix and match analytes from existing panels with our assay designer.
Get in touch with one of our FirePlex® specialists.
FirePlex® is a registered trademark in the United States and is an unregistered trademark elsewhere.
Find out more about our fully customizable and highly sensitive multiplex panels that allow you to analyze up to 70 analytes simultaneously per well on standard flow cytometers.
Ideal for discovery, screening, and profiling projects, high-throughput Fireplex immunoassays offer you a fast, easy, and cost-effective tool to analyze thousands of samples rapidly.
Find out how FirePlex particle technology works with your flow cytometer or high content imager to allow highly sensitive, true multiplex analysis.
Our in-house team of experts will analyze your samples, supportting you throughout the entire process: from tailoring the initial experimental design to providing in-depth data analysis.
Download software and guides for the FirePlex Analysis Workbench.
Select analytes of interest from existing panels with Mix and Match assay designer to build the assay that best meets your needs.
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