FirePlex® Immunoassays

​​​​Abcam's proprietary FirePlex particle technology is ideal for multiplexing protein targets. The bar-coded hydrogel particles allow the bulk immobilization of capture antibody, providing enhanced binding capacity in comparison to surface-functionalization techniques. The flexibility of the hydrogel matrix reduces steric constraints on the binding while enhancing binding kinetics in a planar array format, making assay readouts possible both on flow cytometers as well as High Content Imagers.

FirePlex Immunoassays are offered in three complementary formats for convenient scale-up of samples/compounds for screening:

FirePlex-96 assay:
- Ideal for target screen in 96-well plate format
- pg sensitivity and 5-log dynamic range
- Simple, straight forward workflow
- Readout on a flow cytometer

FirePlex-384 assay:
- Ideal for high throughput screening in 384-well plate format
- Small sample volume of 6.25 µl
- No-wash, two-step - automation friendly
- Readout on High Content Imager

FirePlex-1536 assay:
- Ideal for high throughput screening in 1536-well plate format
- Small sample volume of 2.5 µl
- No-wash, one-step - automation recommended
- Readout on High Content Imager​

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 ​*Volumes listed for 1:2 dilution.

Mix and match analytes from existing panels in our assay designer.

To enquire about FirePlex panels, contact us at fireplexsales@abcam.com.