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Home Multiplex Assays

FirePlex® Immunoassays

Product
  • Pre-designed Assays
  • Core Reagent Kits
  • Accessories

​​​​Abcam's proprietary FirePlex particle technology is ideal for multiplexing protein targets. The bar-coded hydrogel particles allow the bulk immobilization of capture antibody, providing enhanced binding capacity in comparison to surface-functionalization techniques. The flexibility of the hydrogel matrix reduces steric constraints on the binding while enhancing binding kinetics in a planar array format, making assay readouts possible both on flow cytometers as well as high content imagers.  

We offer FirePlex immunoassays in three main formats:

FirePlex-96 assay: ideal for simple, straightforward workflows in biomarker profiling

- Up to 70-plex in 96-well plate

FirePlex-384 assay: ideal for high-throughput screening

- Up to 10-plex high-throughput in 384-well plate format

- No-wash, two-step - automation friendly

FirePlex-1536 assay: ideal for high-throughput screening

- Up to 10-plex high-throughput in 1536-well plate format

- No-wash, one-step - automation recommended

- FirePlex-96 immunoassay: up to 70-plex in 96-well plate, ideal for biomarker profiling.

- FirePlex-384 and FirePlex-1536 immunoassays: up to 10-plex high-throughput (384- and 1536-well) formats with no-wash, automated workflow.

FirePlex-96 assay: up to 70-plex in 96-well plate, ideal simple, straightforward workflow in biomarker profiling

FirePlex-384 assay:
- Ideal for high throughput screening in 384-well plate format
- No-wash, two-step - automation friendly

FirePlex-1536 assay:
- Ideal for high throughput screening in 1536-well plate format
- No-wash, one-step - automation recommended

FirePlex Assay

Instrumentation

Automation

Sample Input*

Hands-on time

Dynamic Range

Sensitivity

Required

Optional

FirePlex-96

Flow Cytometer; Vacuum plate washer

Liquid handler

Optional

12.5 µl

2.5 hours

5 logs

sub pg - pg

FirePlex-384

High Content imager

Liquid handler

Optional

6.25 µl

30 min

3-4 logs

pg

FirePlex-1536

High Content imager

Liquid handler

Recommended

2.5 µl

1 hour

3 logs

pg

 ​*Volumes listed for 1:2 dilution.

Need something more specific?

Mix and match analytes from existing panels with our assay designer.

Get in touch with one of our FirePlex® specialists. 

FirePlex® is a registered trademark in the United States and is an unregistered trademark elsewhere.

Browse all related products

Features

Fireplex-96 immunoassays

​​​​Find out more about our fully customizable and highly sensitive multiplex panels that allow you to analyze up to 70 analytes simultaneously per well on standard flow cytometers.

High-throughput FirePlex immunoassays

Ideal for discovery, screening, and profiling projects, high-throughput Fireplex immunoassays offer you a fast, easy, and cost-effective tool to analyze thousands of samples rapidly. 

 

FirePlex Core Technology

Find out how FirePlex particle technology works with your flow cytometer or high content imager to allow highly sensitive, true multiplex analysis. ​

​FirePlex assay testing services

Our in-house team of experts will analyze your samples, supportting you throughout the entire process: from tailoring the initial experimental design to providing in-depth data analysis.

 

​FirePlex Analysis Workbench software

Download software and guides for the FirePlex Analysis Workbench.​​​​​​​

Customizable FirePlex panels 

Select analytes of interest from existing panels with Mix and Match assay designer to build the assay that best meets your needs.

1
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