FirePlex® miRNA Platform

​​Multiplex miRNA assays with FirePlex particle technology enables simultaneous profiling of 65 miRNAs directly from small amounts of biofluid or FFPE, without RNA purification or pre-amplification, with assay readout on a standard flow cytometer.

• Small sample input

20ul of serum, plasma, urine, exosomes, single 5 µm FFPE section.

• Extraction-free

Eliminate extraction bias, reduce variability, simplify workflow, and conserve your sample.

• Easy and rapid data analysis with FirePlex Analysis Workbench software

Carry out complex analysis and visualization, as well as export publication-quality heat maps and graphs in under ten minutes.

• No specialized equipment is required

Readout is performed on standard flow cytometers

• Parallel processing of 96 samples

Multiplexed detection of up to 70 targets (65 miRNA and 5 internal controls) in 96-well plate

• A customizable biomarker development platform

Select between pre-designed panels for key research areas, larger discovery panels, or design custom panels to detect any miRNA from any species, whether annotated in miRBase or not.

To learn more about the performance of FirePlex miRNA assays and their comparison with the other platforms, check out the tech notes below:

Benchmarking the FirePlex® miRNA Assay  

MicroRNA profiling using the Multiplex Circulating miRNA assay

Molecular and protocol workflows for FirePlex miRNA assays

Figure 1. Molecular workflow for the Multiplex Circulating miRNA Assay

Figure 2. Protocol workflow for the Multiplex Circulating miRNA Assay

If you're interested in FirePlex products, contact us at fireplexsales@abcam.com for more information.