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Multiplex miRNA assays with FirePlex particle technology enables simultaneous profiling of 65 miRNAs directly from small amounts of biofluid or FFPE, without RNA purification or pre-amplification, with assay readout on a standard flow cytometer.
20ul of serum, plasma, urine, exosomes, single 5 µm FFPE section.
Eliminate extraction bias, reduce variability, simplify workflow, and conserve your sample.
Carry out complex analysis and visualization, as well as export publication-quality heat maps and graphs in under ten minutes.
Readout is performed on standard flow cytometers
Multiplexed detection of up to 70 targets (65 miRNA and 5 internal controls) in 96-well plate
Select between pre-designed panels for key research areas, larger discovery panels, or design custom panels to detect any miRNA from any species, whether annotated in miRBase or not.
To learn more about the performance of FirePlex miRNA assays and their comparison with the other platforms, check out the tech notes below:
Figure 1. Molecular workflow for the Multiplex Circulating miRNA Assay
Figure 2. Protocol workflow for the Multiplex Circulating miRNA Assay
If you're interested in FirePlex products, contact us for more information.
Find out more about how the FirePlex miRNA Assay can benefit your research.
Design a custom particle panel to profile miRNAs of choice for your experiment
Download software and guides for the FirePlex Analysis Workbench.