For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    abhd5cgi-58-antibody-epr12621-ab183739.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Lipids / Lipoproteins Adipose Related Lipid Droplet Protein
Share by email
RecombinantRabMAb

Recombinant Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (2) Submit a question References (13)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
  • Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
  • Flow Cytometry (Intracellular) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
  • Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12621] to Abhd5/CGI-58
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

APC Carrier Free HRP PE

You may also be interested in

Protein
Product image
Recombinant Human Abhd5/CGI-58 protein (ab132557)
Primary
Product image
Anti-SOX11 antibody [EPR8192] (ab134107)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-Abhd5/CGI-58 antibody [EPR12621]
    See all Abhd5/CGI-58 primary antibodies
  • Description

    Rabbit monoclonal [EPR12621] to Abhd5/CGI-58
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2 cells and cell lysates; U87-MG and Human fetal liver cell lysates; Human lipomyoma tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12621
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Adipose Related
    • Lipid Droplet Protein
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-Abhd5/CGI-58 antibody [EPR12621] - BSA and Azide free (ab239001)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
  • Recombinant Protein

    • Recombinant Human Abhd5/CGI-58 protein (ab132557)
  • Related Products

    • Recombinant Human Abhd5/CGI-58 protein (ab132557)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (2)
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.
  • Tissue specificity

    Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).
  • Involvement in disease

    Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS) [MIM:275630]; also called triglyceride storage disease with impaired long-chain fatty acid oxidation or neutral lipid storage disease with ichthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma.
  • Sequence similarities

    Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
  • Developmental stage

    Detected in fetal epidermis from 49 to 135 days estimated gestational age (at protein level).
  • Domain

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localization

    Cytoplasm. Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA.
  • Target information above from: UniProt accession Q8WTS1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 51099 Human
    • Entrez Gene: 67469 Mouse
    • Omim: 604780 Human
    • SwissProt: Q8WTS1 Human
    • SwissProt: Q9DBL9 Mouse
    • Unigene: 19385 Human
    • Unigene: 280254 Mouse
    • Alternative names

      • 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody
      • ABHD5 antibody
      • ABHD5_HUMAN antibody
      • Abhydrolase domain containing 5 antibody
      • Abhydrolase domain containing protein 5 antibody
      • Abhydrolase domain-containing protein 5 antibody
      • CDS antibody
      • CGI 58 antibody
      • CGI58 antibody
      • CGI58 protein antibody
      • IECN2 antibody
      • Lipid droplet-binding protein CGI-58 antibody
      • MGC8731 antibody
      • NCIE2 antibody
      see all

    Images

    • Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      All lanes : Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 39 kDa

    • Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739) at 1/1000 dilution + Human fetal liver lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 39 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

      Immunohistochemical analysis of paraffin-embedded Human lipomyoma tissue labeling Abhd5/CGI-58 with ab183739 at 1/2000 dilution. The slide is counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Flow Cytometry (Intracellular) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

      Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling Abhd5/CGI-58 with ab183739 at 1/200 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control. 

       

       

    • Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (13)

    Publishing research using ab183739? Please let us know so that we can cite the reference in this datasheet.

    ab183739 has been referenced in 13 publications.

    • Cero C  et al. ß3-Adrenergic receptors regulate human brown/beige adipocyte lipolysis and thermogenesis. JCI Insight 6:N/A (2021). PubMed: 34100382
    • Arumugam MK  et al. Elevated S-adenosylhomocysteine induces adipocyte dysfunction to promote alcohol-associated liver steatosis. Sci Rep 11:14693 (2021). PubMed: 34282217
    • Zhou Q & Sun Y Circular RNA cMras Suppresses the Progression of Lung Adenocarcinoma Through ABHD5/ATGL Axis Using NF-?B Signaling Pathway. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32822232
    • Ryan BJ  et al. Moderate-Intensity Exercise and High-Intensity Interval Training Affect Insulin Sensitivity Similarly in Obese Adults. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32492705
    • Wu H  et al. Evidence for a Novel Regulatory Interaction Involving Cyclin D1, Lipid Droplets, Lipolysis, and Cell Cycle Progression in Hepatocytes. Hepatol Commun 3:406-422 (2019). PubMed: 30859152
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Abhd5/CGI-58 antibody [EPR12621]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (white adipose tissue)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Loading amount
    5 µg
    Specification
    white adipose tissue
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 25 2022

    Western blot abreview for Anti-Abhd5/CGI-58 antibody [EPR12621]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (adipose tissue)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    adipose tissue
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Helle Zibrandtsen

    Verified customer

    Submitted Mar 11 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.