Recombinant Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
![Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)](https://www.abcam.com/ps/products/183/ab183739/Images/ab183739-215960-anti-abhd5-cgi-58-antibody-epr12621-western-blot.jpg "Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12621] to Abhd5/CGI-58
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Abhd5/CGI-58 antibody [EPR12621]
See all Abhd5/CGI-58 primary antibodies -
Description
Rabbit monoclonal [EPR12621] to Abhd5/CGI-58 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2 cells and cell lysates; U87-MG and Human fetal liver cell lysates; Human lipomyoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12621 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/200.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
|
| IHC-P |
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
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Flow Cyt (Intra)
1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa). |
|
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. -
Tissue specificity
Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level). -
Involvement in disease
Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS) [MIM:275630]; also called triglyceride storage disease with impaired long-chain fatty acid oxidation or neutral lipid storage disease with ichthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma. -
Sequence similarities
Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. -
Developmental stage
Detected in fetal epidermis from 49 to 135 days estimated gestational age (at protein level). -
Domain
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
Cellular localization
Cytoplasm. Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA. - Information by UniProt
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Database links
- Entrez Gene: 51099 Human
- Entrez Gene: 67469 Mouse
- Omim: 604780 Human
- SwissProt: Q8WTS1 Human
- SwissProt: Q9DBL9 Mouse
- Unigene: 19385 Human
- Unigene: 280254 Mouse
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Alternative names
- 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody
- ABHD5 antibody
- ABHD5_HUMAN antibody
see all
Images
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Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)All lanes : Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDa -
![Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)](https://www.abcam.com/ps/products/183/ab183739/Images/ab183739-215958-anti-abhd5-cgi-58-antibody-epr12621-western-blot.jpg)
Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739) at 1/1000 dilution + Human fetal liver lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 39 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)](https://www.abcam.com/ps/products/183/ab183739/Images/ab183739-215957-anti-abhd5-cgi-58-antibody-epr12621-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)Immunohistochemical analysis of paraffin-embedded Human lipomyoma tissue labeling Abhd5/CGI-58 with ab183739 at 1/2000 dilution. The slide is counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)](https://www.abcam.com/ps/products/183/ab183739/Images/ab183739-215956-anti-abhd5-cgi-58-antibody-epr12621-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling Abhd5/CGI-58 with ab183739 at 1/200 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.
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![Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)](https://www.abcam.com/ps/products/183/ab183739/Images/ab183739-5-benefits-of-recombinant-antibodies.png)
Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (13)
ab183739 has been referenced in 13 publications.
- Cero C et al. ß3-Adrenergic receptors regulate human brown/beige adipocyte lipolysis and thermogenesis. JCI Insight 6:N/A (2021). PubMed: 34100382
- Arumugam MK et al. Elevated S-adenosylhomocysteine induces adipocyte dysfunction to promote alcohol-associated liver steatosis. Sci Rep 11:14693 (2021). PubMed: 34282217
- Zhou Q & Sun Y Circular RNA cMras Suppresses the Progression of Lung Adenocarcinoma Through ABHD5/ATGL Axis Using NF-?B Signaling Pathway. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32822232
- Ryan BJ et al. Moderate-Intensity Exercise and High-Intensity Interval Training Affect Insulin Sensitivity Similarly in Obese Adults. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32492705
- Wu H et al. Evidence for a Novel Regulatory Interaction Involving Cyclin D1, Lipid Droplets, Lipolysis, and Cell Cycle Progression in Hepatocytes. Hepatol Commun 3:406-422 (2019). PubMed: 30859152
