RecombinantRabMAb

Recombinant Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

Reviews (1) Submit a question References (10)
Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
  • Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
  • Flow Cytometry (Intracellular) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
  • Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12621] to Abhd5/CGI-58
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Abhd5/CGI-58 antibody [EPR12621]
    See all Abhd5/CGI-58 primary antibodies

  • Description

    Rabbit monoclonal [EPR12621] to Abhd5/CGI-58

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2 cells and cell lysates; U87-MG and Human fetal liver cell lysates; Human lipomyoma tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.

  • Tissue specificity

    Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).

  • Involvement in disease

    Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS) [MIM:275630]; also called triglyceride storage disease with impaired long-chain fatty acid oxidation or neutral lipid storage disease with ichthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma.

  • Sequence similarities

    Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.

  • Developmental stage

    Detected in fetal epidermis from 49 to 135 days estimated gestational age (at protein level).

  • Domain

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.

  • Cellular localization

    Cytoplasm. Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA.
  • Information by UniProt

  • Database links

    • Alternative names

      • 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody
      • ABHD5 antibody
      • ABHD5_HUMAN antibody
      • Abhydrolase domain containing 5 antibody
      • Abhydrolase domain containing protein 5 antibody
      • Abhydrolase domain-containing protein 5 antibody
      • CDS antibody
      • CGI 58 antibody
      • CGI58 antibody
      • CGI58 protein antibody
      • IECN2 antibody
      • Lipid droplet-binding protein CGI-58 antibody
      • MGC8731 antibody
      • NCIE2 antibody
      see all

    Images

    • Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      All lanes : Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 39 kDa

    • Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739) at 1/1000 dilution + Human fetal liver lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 39 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

      Immunohistochemical analysis of paraffin-embedded Human lipomyoma tissue labeling Abhd5/CGI-58 with ab183739 at 1/2000 dilution. The slide is counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Flow Cytometry (Intracellular) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

      Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling Abhd5/CGI-58 with ab183739 at 1/200 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control. 

       

       

    • Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

    References (10)

    Publishing research using ab183739? Please let us know so that we can cite the reference in this datasheet.

    ab183739 has been referenced in 10 publications.

    • Zhou Q & Sun Y Circular RNA cMras Suppresses the Progression of Lung Adenocarcinoma Through ABHD5/ATGL Axis Using NF-?B Signaling Pathway. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32822232
    • Ryan BJ  et al. Moderate-Intensity Exercise and High-Intensity Interval Training Affect Insulin Sensitivity Similarly in Obese Adults. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32492705
    • Wu H  et al. Evidence for a Novel Regulatory Interaction Involving Cyclin D1, Lipid Droplets, Lipolysis, and Cell Cycle Progression in Hepatocytes. Hepatol Commun 3:406-422 (2019). PubMed: 30859152
    • Ramshanker N  et al. Effects of short-term prednisolone treatment on indices of lipolysis and lipase signaling in abdominal adipose tissue in healthy humans. Metabolism 99:1-10 (2019). PubMed: 31260678
    • Riis S  et al. Molecular adaptations in human subcutaneous adipose tissue after ten weeks of endurance exercise training in healthy males. J Appl Physiol (1985) N/A:N/A (2018). PubMed: 30571288
    • Mitra R  et al. Positive regulation of prostate cancer cell growth by lipid droplet forming and processing enzymes DGAT1 and ABHD5. BMC Cancer 17:631 (2017). PubMed: 28877685
    • Voss TS  et al. Effects of insulin-induced hypoglycaemia on lipolysis rate, lipid oxidation and adipose tissue signalling in human volunteers: a randomised clinical study. Diabetologia 60:143-152 (2017). PubMed: 27734104
    • Rittig N  et al. Regulation of Lipolysis and Adipose Tissue Signaling during Acute Endotoxin-Induced Inflammation: A Human Randomized Crossover Trial. PLoS One 11:e0162167 (2016). PubMed: 27627109
    • Christensen B  et al. Erythropoietin does not activate erythropoietin receptor signaling or lipolytic pathways in human subcutaneous white adipose tissue in vivo. Lipids Health Dis 15:160 (2016). WB ; Human . PubMed: 27640183
    • Abreu-Vieira G  et al. Cidea improves the metabolic profile through expansion of adipose tissue. Nat Commun 6:7433 (2015). PubMed: 26118629

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Abhd5/CGI-58 antibody [EPR12621]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (adipose tissue)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    adipose tissue
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More

    Ms. Helle Zibrandtsen

    Verified customer

    Submitted Mar 11 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com