Recombinant Anti-ADAM9 antibody [EPR21924-164] (ab218242)
![Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)](https://www.abcam.com/ps/products/218/ab218242/Images/ab218242-389422-anti-adam9-antibody-epr21924-164-western-blot-human-knockout-lysate.jpg "Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21924-164] to ADAM9
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ADAM9 antibody [EPR21924-164]
See all ADAM9 primary antibodies -
Description
Rabbit monoclonal [EPR21924-164] to ADAM9 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, HeLa, WI-38, MCF7, U-87 MG and A549 whole cell lysates. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21924-164 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
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| WB |
1/1000. Detects a band of approximately 75, 100 kDa (predicted molecular weight: 91 kDa).
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| Notes |
|---|
|
IP
1/30. |
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WB
1/1000. Detects a band of approximately 75, 100 kDa (predicted molecular weight: 91 kDa). |
Target
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Function
Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP. -
Tissue specificity
Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. -
Involvement in disease
Cone-rod dystrophy 9 -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8754 Human
- Omim: 602713 Human
- SwissProt: Q13443 Human
- Unigene: 591852 Human
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Alternative names
- ADAM 9 antibody
- ADAM metallopeptidase domain 9 antibody
- Adam9 antibody
see all
Images
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Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)All lanes : Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ADAM9 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 91 kDa
Observed band size: 100,75 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab218242 observed at 100,75 kDa. Red - loading control ab8245 observed at 36 kDa.
ab218242 Anti-ADAM9 antibody [EPR21924-164] was shown to specifically react with ADAM9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265484 (knockout cell lysate ab257814) was used. Wild-type and ADAM9 knockout samples were subjected to SDS-PAGE. ab218242 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)](https://www.abcam.com/ps/products/218/ab218242/Images/ab218242-316600-anti-adam9-antibody-epr21924-164-western-blot.jpg)
Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)All lanes : Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution
Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : WI-38 (human fetal lung fibroblast), whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 6 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 7 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 91 kDa
Observed band size: 100,75 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure times:
Lanes 1-5: 3 minutes
Lane 6: 48 seconds
Lane 7: 114 secondsThe molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367).
We recommend that customers avoid boiling their samples to prevent protein aggregation.
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![Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] (ab218242)](https://www.abcam.com/ps/products/218/ab218242/Images/ab218242-316599-anti-adam9-antibody-epr21924-164-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] (ab218242)ADAM9 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma cell) whole cell lysate with ab218242 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218242 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10μg (input).
Lane 2: ab218242 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218242 in HeLa whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.The conformational epitope maybe changed after cleavage and this antibody has better affinity to the pro-form.
The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367).
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![Anti-ADAM9 antibody [EPR21924-164] (ab218242)](https://www.abcam.com/ps/products/218/ab218242/Images/ab218242-4-benefits-of-recombinant-antibodies.png)
Anti-ADAM9 antibody [EPR21924-164] (ab218242)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab218242 has been referenced in 2 publications.
- Gao H et al. Depletion of hsa_circ_0000144 Suppresses Oxaliplatin Resistance of Gastric Cancer Cells by Regulating miR-502-5p/ADAM9 Axis. Onco Targets Ther 14:2773-2787 (2021). PubMed: 33907420
- Lu W et al. Tetraspanin CD9 interacts with a-secretase to enhance its oncogenic function in pancreatic cancer. Am J Transl Res 12:5525-5537 (2020). PubMed: 33042435