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    adam9-antibody-epr21924-164-ab218242.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ADAM9 antibody [EPR21924-164] (ab218242)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (2)

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Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)
  • Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)
  • Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] (ab218242)
  • Anti-ADAM9 antibody [EPR21924-164] (ab218242)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21924-164] to ADAM9
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-ADAM9 antibody [EPR21924-164]
    See all ADAM9 primary antibodies
  • Description

    Rabbit monoclonal [EPR21924-164] to ADAM9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, HeLa, WI-38, MCF7, U-87 MG and A549 whole cell lysates. IP: HeLa whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21924-164
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-ADAM9 antibody [EPR21924-164] - BSA and Azide free (ab238647)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ADAM9 knockout HeLa cell line (ab265484)
  • KO cell lysates

    • Human ADAM9 knockout HeLa cell lysate (ab257814)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab218242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/30.
WB
1/1000. Detects a band of approximately 75, 100 kDa (predicted molecular weight: 91 kDa).
Notes
IP
1/30.
WB
1/1000. Detects a band of approximately 75, 100 kDa (predicted molecular weight: 91 kDa).

Target

  • Function

    Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP.
  • Tissue specificity

    Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart.
  • Involvement in disease

    Cone-rod dystrophy 9
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q13443 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8754 Human
    • Omim: 602713 Human
    • SwissProt: Q13443 Human
    • Unigene: 591852 Human
    • Alternative names

      • ADAM 9 antibody
      • ADAM metallopeptidase domain 9 antibody
      • Adam9 antibody
      • ADAM9_HUMAN antibody
      • Cellular disintegrin-related protein antibody
      • Cone rod dystrophy 9 antibody
      • CORD9 antibody
      • Disintegrin and metalloproteinase domain-containing protein 9 antibody
      • MCMP antibody
      • MDC9 antibody
      • Meltrin-gamma antibody
      • Metalloprotease/disintegrin/cysteine-rich protein 9 antibody
      • Mltng antibody
      • Myeloma cell metalloproteinase antibody
      see all

    Images

    • Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)
      Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)
      All lanes : Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : ADAM9 knockout HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 91 kDa
      Observed band size: 100,75 kDa why is the actual band size different from the predicted?



      Lanes 1-3: Merged signal (red and green). Green - ab218242 observed at 100,75 kDa. Red - loading control ab8245 observed at 36 kDa.

      ab218242 Anti-ADAM9 antibody [EPR21924-164] was shown to specifically react with ADAM9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265484 (knockout cell lysate ab257814) was used. Wild-type and ADAM9 knockout samples were subjected to SDS-PAGE. ab218242 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

       

    • Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)
      Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)
      All lanes : Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution

      Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 3 : WI-38 (human fetal lung fibroblast), whole cell lysate
      Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
      Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
      Lane 6 : A549 (human lung carcinoma epithelial cell), whole cell lysate
      Lane 7 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 91 kDa
      Observed band size: 100,75 kDa why is the actual band size different from the predicted?



      Blocking/diluting buffer and concentration:  5% NFDM/TBST 

      Exposure times:
      Lanes 1-5: 3 minutes
      Lane 6: 48 seconds
      Lane 7: 114 seconds

      The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367).

      We recommend that customers avoid boiling their samples to prevent protein aggregation.

    • Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] (ab218242)
      Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] (ab218242)

      ADAM9 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma cell) whole cell lysate with ab218242 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218242 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

      Lane 1: HeLa whole cell lysate 10μg (input).
      Lane 2: ab218242 IP in HeLa whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218242 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 3 minutes.

      The conformational epitope maybe changed after cleavage and this antibody has better affinity to the pro-form.

      The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367).

    • Anti-ADAM9 antibody [EPR21924-164] (ab218242)
      Anti-ADAM9 antibody [EPR21924-164] (ab218242)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (2)

    Publishing research using ab218242? Please let us know so that we can cite the reference in this datasheet.

    ab218242 has been referenced in 2 publications.

    • Gao H  et al. Depletion of hsa_circ_0000144 Suppresses Oxaliplatin Resistance of Gastric Cancer Cells by Regulating miR-502-5p/ADAM9 Axis. Onco Targets Ther 14:2773-2787 (2021). PubMed: 33907420
    • Lu W  et al. Tetraspanin CD9 interacts with a-secretase to enhance its oncogenic function in pancreatic cancer. Am J Transl Res 12:5525-5537 (2020). PubMed: 33042435

    Customer reviews and Q&As

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