Recombinant Anti-Androgen Receptor antibody [EP670Y] (ab52615)
![Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)](https://www.abcam.com/ps/products/52/ab52615/Images/ab52615-394150-anti-androgen-receptor-antibody-ep670y-western-blot-testis-rat-mouse.jpg "Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP670Y] to Androgen Receptor
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Androgen Receptor antibody [EP670Y]
See all Androgen Receptor primary antibodies -
Description
Rabbit monoclonal [EP670Y] to Androgen Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
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Positive control
- WB: PC3 whole cell lysate (ab3954), LnCaP and 22Rv1 cell lysate and mouse and rat testis tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP670Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
We recommend to use 1%SDS Hot lysis prepare method. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 99 kDa. We recommend to use 1%SDS Hot lysis prepare method. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Function
Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. -
Tissue specificity
Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107). -
Involvement in disease
Androgen insensitivity syndrome
Spinal and bulbar muscular atrophy X-linked 1
Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
Androgen insensitivity, partial -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain. -
Post-translational
modificationsSumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation. -
Cellular localization
Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1. - Information by UniProt
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Database links
- Entrez Gene: 367 Human
- Entrez Gene: 11835 Mouse
- Entrez Gene: 24208 Rat
- Omim: 313700 Human
- SwissProt: P10275 Human
- SwissProt: P19091 Mouse
- SwissProt: P15207 Rat
- Unigene: 76704 Human
see all -
Form
There are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45. -
Alternative names
- AIS antibody
- ANDR_HUMAN antibody
- Androgen nuclear receptor variant 2 antibody
see all
Images
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Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)All lanes : Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/1000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lane 3 : Mouse liver lysate
Lane 4 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Diluting buffer: 5% NFDM/TBST
Loading control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)
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![Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)](https://www.abcam.com/ps/products/52/ab52615/Images/ab52615-280606-anti-androgen-receptor-antibody-ep670y-western-blot.jpg)
Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)All lanes : Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/1000 dilution
Lane 1 : LnCAP whole cell lysate (positive control)
Lane 2 : PC3 whole cell lysate (negative control)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.Lanes 1 - 2: Merged signal (red and green). Green - ab52615 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52615 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.
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![Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)](https://www.abcam.com/ps/products/52/ab52615/Images/ab52615-309668-anti-androgen-receptor-antibody-ep670y-western-blot.jpg)
Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)All lanes : Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/5000 dilution (Purified)
Lane 1 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
Lane 2 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol here (downloadable copy).
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![Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)](https://www.abcam.com/ps/products/52/ab52615/Images/ab52615-64958-anti-androgen-receptor-antibody-ep670y-western-blot.jpg)
Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/10000 dilution (unpurified) + LnCaP cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 99 kDa
Observed band size: 99 kDa -
![Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)](https://www.abcam.com/ps/products/52/ab52615/Images/ab52615-253914-anti-androgen-receptor-antibody-ep670y-western-blot.jpg)
Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)All lanes : Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/2000 dilution (purified)
Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-Androgen Receptor antibody [EP670Y] (ab52615)](https://www.abcam.com/ps/products/52/ab52615/Images/ab52615-8-benefits-of-recombinant-antibodies.png)
Anti-Androgen Receptor antibody [EP670Y] (ab52615)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab52615 has been referenced in 22 publications.
- Liva SG et al. Overcoming resistance to anabolic SARM therapy in experimental cancer cachexia with an HDAC inhibitor. EMBO Mol Med 12:e9910 (2020). PubMed: 31930715
- Wasmuth EV et al. Modulation of androgen receptor DNA binding activity through direct interaction with the ETS transcription factor ERG. Proc Natl Acad Sci U S A 117:8584-8592 (2020). PubMed: 32220959
- Williams ES et al. Androgen-Dependent Excitability of Mouse Ventral Hippocampal Afferents to Nucleus Accumbens Underlies Sex-Specific Susceptibility to Stress. Biol Psychiatry 87:492-501 (2020). PubMed: 31601425
- Dauki AM et al. Transcriptionally Active Androgen Receptor Splice Variants Promote Hepatocellular Carcinoma Progression. Cancer Res 80:561-575 (2020). PubMed: 31685543
- Acosta-Lopez S et al. The androgen receptor expression and its activity have different relationships with prognosis in hepatocellular carcinoma. Sci Rep 10:22046 (2020). PubMed: 33328560