Recombinant Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)
![Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)](https://www.abcam.com/ps/products/254/ab254262/Images/ab254262-8-anti-asialoglycoprotein-receptor-1-hl1-epr22642-17-western-blot-human.jpg "Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22642-17] to Asialoglycoprotein Receptor 1/HL-1
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17]
See all Asialoglycoprotein Receptor 1/HL-1 primary antibodies -
Description
Rabbit monoclonal [EPR22642-17] to Asialoglycoprotein Receptor 1/HL-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human adult liver tissue lysate; HepG2 cell lysate. IHC-P: Human liver and liver cancer tissue. ICC/IF: HepG2 cells. Flow: HepG2 cells. IP: HepG2 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22642-17 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 40-50 kDa (predicted molecular weight: 33 kDa).
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| ICC/IF |
1/100.
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Flow Cyt |
1/500.
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| IP |
1/30.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 40-50 kDa (predicted molecular weight: 33 kDa). |
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ICC/IF
1/100. |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Flow Cyt
1/500. |
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IP
1/30. |
Target
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Function
Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface. -
Tissue specificity
Expressed exclusively in hepatic parenchymal cells. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsPhosphorylated on a cytoplasmic Ser residue. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 432 Human
- Omim: 108360 Human
- SwissProt: P07306 Human
- Unigene: 12056 Human
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Alternative names
- ASGP-R 1 antibody
- ASGPR 1 antibody
- ASGPR antibody
see all
Images
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Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)All lanes : Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262) at 1/1000 dilution
Lane 1 : Human adult liver tissue lysate
Lane 2 : Human brain tissue lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 33 kDa
Observed band size: 40-50 kDa why is the actual band size different from the predicted?Negative control: human brain.
The diffuse bands between 40-50 kDa are likely due to glycosylation (PMID:30965276).
The expression profile is consistent with what has been described in the literature (PMID:27241665, 29858079).
Blocking/diluting buffer and concentration: 5% NFDM/TBST
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![Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)](https://www.abcam.com/ps/products/254/ab254262/Images/ab254262-7-anti-asialoglycoprotein-receptor-1-hl1-epr22642-17-ip-hepg2-human.jpg)
Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)Asialoglycoprotein Receptor 1/HL-1 antibody was immunoprecipitated from 0.35 mg HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg with ab254262 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254262 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
Lane 2: ab254262 IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254262 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7 seconds.
The band at around 100kDa is described as dimers (PMID: 7663158).
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![Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)](https://www.abcam.com/ps/products/254/ab254262/Images/ab254262-6-anti-asialoglycoprotein-receptor-1-hl1-epr22642-17-immunocytochem-hepg2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell line) cells labelling Asialoglycoprotein Receptor 1/HL-1 antibody with ab254262 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in HepG2 cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counter stain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)](https://www.abcam.com/ps/products/254/ab254262/Images/ab254262-5-anti-asialoglycoprotein-receptor-1-hl1-epr22642-17-ihc-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Asialoglycoprotein Receptor 1/HL-1 antibody with ab254262 at 1/1000 dilution (0.5 μg/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human liver (PMID:23979840, 26422581, 27241665) is observed.
The section was incubated with ab254262 for 15 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)](https://www.abcam.com/ps/products/254/ab254262/Images/ab254262-1-anti-asialoglycoprotein-1-hl1-antibody-epr22642-17-ihc-liver-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Asialoglycoprotein Receptor 1/HL-1 antibody with ab254262 at 1/1000 dilution (0.5 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human liver cancer (PMID:23979840,26422581,27241665) is observed.
The section was incubated with ab254262 for 15 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)](https://www.abcam.com/ps/products/254/ab254262/Images/ab254262-4-anti-asialoglycoprotein-receptor-1-hl1-epr22642-17-ihc-colon-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)Negative control: No staining in human colon (PMID:23979840).
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Asialoglycoprotein Receptor 1/HL-1 antibody with ab254262 at 1/1000 dilution (0.5 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab254262 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)](https://www.abcam.com/ps/products/254/ab254262/Images/ab254262-3-anti-asialoglycoprotein-receptor-1-hl1-epr22642-17-ihc-brain-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)Negative control: No staining in human cerebrum (PMID:23979840)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Asialoglycoprotein Receptor 1/HL-1 antibody with ab254262 at 1/1000 dilution (0.5 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab254262 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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![Flow Cytometry - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)](https://www.abcam.com/ps/products/254/ab254262/Images/ab254262-2-anti-asialoglycoprotein-receptor-1-hl1-epr22642-17-flowcytometry-hepg2.jpg)
Flow Cytometry - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)Flow cytometric analysis of HepG2 (Human hepatocellular carcinoma epithelial cell line) cells labelling Asialoglycoprotein Receptor 1/HL-1 antibody with ab254262 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)](https://www.abcam.com/ps/products/254/ab254262/Images/ab254262-9-benefits-of-recombinant-antibodies.png)
Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab254262 has been referenced in 1 publication.
- Li N et al. Astragaloside IV alleviates silica-induced pulmonary fibrosis via inactivation of the TGF-ß1/Smad2/3 signaling pathway. Int J Mol Med 47:N/A (2021). PubMed: 33448318
