Recombinant Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)
![Western blot - Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)](https://www.abcam.com/ps/products/77/ab77236/Images/ab77236-92966-anti-band-3-ae-1-phospho-y359-antibody-epr1427y-western-blot.jpg "Western blot - Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1427Y] to Band 3/AE 1 (phospho Y359)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y]
See all Band 3/AE 1 primary antibodies -
Description
Rabbit monoclonal [EPR1427Y] to Band 3/AE 1 (phospho Y359) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Band 3/AE 1 (phospho Y359).
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Positive control
- Red blood cell lysates treated with pervanadate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1427Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/50000. Predicted molecular weight: 102 kDa.
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| Notes |
|---|
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WB
1/50000. Predicted molecular weight: 102 kDa. |
Application notes
Is unsuitable for Flow Cyt or IP.
Target
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Function
Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine. -
Tissue specificity
Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871). -
Involvement in disease
Ovalocytosis, Southeast Asian
Spherocytosis 4
Renal tubular acidosis, distal, autosomal dominant
Renal tubular acidosis, distal, with hemolytic anemia
Renal tubular acidosis, distal, with normal red cell morphology
Cryohydrocytosis -
Sequence similarities
Belongs to the anion exchanger (TC 2.A.31) family. -
Post-translational
modificationsPhosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
N-glycosylated. -
Cellular localization
Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney. - Information by UniProt
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Database links
- Entrez Gene: 6521 Human
- Omim: 109270 Human
- SwissProt: P02730 Human
- Unigene: 210751 Human
- Unigene: 443948 Human
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Alternative names
- AE 1 antibody
- AE1 antibody
- Anion exchange protein 1 antibody
see all
Images
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Western blot - Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)All lanes : Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236) at 1/50000 dilution
Lane 1 : Red blood cell lysate, untreated.
Lane 2 : Red blood cell lysate treated with pervanadate.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDa -
![Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)](https://www.abcam.com/ps/products/77/ab77236/Images/ab77236-2-benefits-of-recombinant-antibodies.png)
Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab77236 has not yet been referenced specifically in any publications.