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  1. Link

    band-3ae-1-phospho-y359-antibody-epr1427y-ab77236.pdf

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RecombinantRabMAb

Recombinant Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)

  • Datasheet
  • SDS
Submit a review Q&A (6)

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Western blot - Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)
  • Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1427Y] to Band 3/AE 1 (phospho Y359)
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y]
    See all Band 3/AE 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1427Y] to Band 3/AE 1 (phospho Y359)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Band 3/AE 1 (phospho Y359).

  • Positive control

    • Red blood cell lysates treated with pervanadate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1427Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Cardiovascular
    • Blood
    • Other

Associated products

  • Alternative Versions

    • Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] - BSA and Azide free (ab247492)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Band 3/AE 1 protein (Tagged) (ab235968)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab77236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/50000. Predicted molecular weight: 102 kDa.
Notes
WB
1/50000. Predicted molecular weight: 102 kDa.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
  • Tissue specificity

    Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
  • Involvement in disease

    Ovalocytosis, Southeast Asian
    Spherocytosis 4
    Renal tubular acidosis, distal, autosomal dominant
    Renal tubular acidosis, distal, with hemolytic anemia
    Renal tubular acidosis, distal, with normal red cell morphology
    Cryohydrocytosis
  • Sequence similarities

    Belongs to the anion exchanger (TC 2.A.31) family.
  • Post-translational
    modifications

    Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
    Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
    N-glycosylated.
  • Cellular localization

    Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
  • Target information above from: UniProt accession P02730 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6521 Human
    • Omim: 109270 Human
    • SwissProt: P02730 Human
    • Unigene: 210751 Human
    • Unigene: 443948 Human
    • Alternative names

      • AE 1 antibody
      • AE1 antibody
      • Anion exchange protein 1 antibody
      • Anion exchanger 1 antibody
      • B3AT_HUMAN antibody
      • Band 3 anion transport protein antibody
      • Band 3 antibody
      • BND3 antibody
      • CD233 antibody
      • DI antibody
      • Diego blood group antibody
      • EMPB3 antibody
      • EPB3 antibody
      • Erythrocyte membrane protein band 3 antibody
      • Erythroid anion exchange protein antibody
      • FR antibody
      • Froese blood group antibody
      • RTA1A antibody
      • SLC4A1 antibody
      • Solute carrier family 4 anion exchanger member 1 antibody
      • Solute carrier family 4 member 1 antibody
      • SW antibody
      • Swann blood group antibody
      • Waldner blood group antibody
      • WD antibody
      • WD1 antibody
      • WR antibody
      • Wright blood group antibody
      see all

    Images

    • Western blot - Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)
      Western blot - Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)
      All lanes : Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236) at 1/50000 dilution

      Lane 1 : Red blood cell lysate, untreated.
      Lane 2 : Red blood cell lysate treated with pervanadate.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 102 kDa
      Observed band size: 102 kDa

    • Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)
      Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab77236? Please let us know so that we can cite the reference in this datasheet.

    ab77236 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Question

    I am trying to submit the abreview but I am stranded in what abbreviation I should choose. I can't find a list of abbreviations on the site. I did an immune fluorescence assay, could you tell me what abbreviation that is.
    cheers

    Read More

    Abcam community

    Verified customer

    Asked on May 14 2012

    Answer

    Thank you for contacting us. I am sorry for the confusion. Please select according to the following options.

    If the antibody was used in Immunofluorescence on cells then select ICC/IF.

    If on frozen tissue then select IHC-Fr

    If used on paraffin embedded tissues then select IHC-P

    If used in paraformaldehyde perfused and frozen sections then select IHC-FoFR.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on May 14 2012

    Question

    we have tested the antibody in Macacca mulatta like we agreed on. However I seem to need an emailadress or customernumber and password. The person that does all the purchases here doesn't have these details, I think she uses the fax. Do you have these details for me so I can submit the review?
    Thank you

    Read More

    Abcam community

    Verified customer

    Asked on May 11 2012

    Answer

    Thank you for contacting us.

    Your customer number is XXXXXXXX.

    Please use the same email address to retrieve the password by clicking forget password.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on May 11 2012

    Question

    Do I include the discountcode in this order?
    greetings

    Read More

    Abcam community

    Verified customer

    Asked on Apr 19 2012

    Answer

    Thank you for your email.

    You need to write the discount code when submitting the Abreview in additional notes box. Please buy the antibody without any hesitation.

    Best of luck for your research.

    Read More

    Abcam Scientific Support

    Answered on Apr 19 2012

    Question

    We would like to do the Abreview 100% testing discount. I asume we should just order then now and you will provide us with a code when we upload the abreview?
    best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 18 2012

    Answer

    Thank you for accepting my offer.

    Unfortunately you would need the discount code before the purchase. Could you tell me which antibody you would like to buy?

    Read More

    Abcam Scientific Support

    Answered on Apr 18 2012

    Question

    thanks for answering our inquiry. Denise forwarded me your e-mail

    Please find attached the Band 3 sequence of macaca mulatta for the further search you proposed.

    Best wishes,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 11 2012

    Answer

    Thank you for contacting us.

    The macaca Band 3 protein is about 94% homologous to the human protein. It seems that the anti human Band 3 antibodies will cross react with Macaca proteins also.

    You can choose any of the anti Band 3 antibodies from the website. Please be advised that Macaca is not a tested species so we could not 100% confirm the x-reactivity. The use of this antibody with macaca is not guaranteed however we are happy to provide the Abreview 100% testing discount if you are interested in using any of these antibody with Macaca mulata species. Please click the link for more info.

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

    Let me know if you are interested in my offer. I will be happy sending you the code.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Apr 11 2012

    Question

    Looking for anti Band 3 specific to extra cellular portion of protein.
    - Would like to use abwith Rhesus monkey sample

    Read More

    Abcam community

    Verified customer

    Asked on Apr 05 2012

    Answer

    Thank you very much for your phone call.

    We currently do not have Rhesus Monkey Band 3 specific antibodies. The Band 3 antibodies we have are ab11012, ab77236, ab78067, ab104998, ab108414, ab125070 and they seems specific to N-terminal cytoplasmic domain of the protein.

    As discussed please send me the sequence link of Rhesus Band 3 protein, I will then do the further search.

    I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Apr 05 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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