Recombinant Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
![Western blot - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)](https://www.abcam.com/ps/products/238/ab238124/Images/ab238124-1-anti-caspase-4-antibody-epr20921-83-western-blot-human.jpg "Western blot - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20921-83] to Caspase-4
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Caspase-4 antibody [EPR20921-83]
See all Caspase-4 primary antibodies -
Description
Rabbit monoclonal [EPR20921-83] to Caspase-4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: THP-1, IM-9 and KARPAS-299 whole cell lysate. Flow Cyt (intra): THP-1 and IM-9 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20921-83 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Predicted molecular weight: 43 kDa.
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| Flow Cyt (Intra) |
1/60.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 43 kDa. |
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Flow Cyt (Intra)
1/60. |
Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. -
Tissue specificity
Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8. - Information by UniProt
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Database links
- Entrez Gene: 837 Human
- Omim: 602664 Human
- SwissProt: P49662 Human
- Unigene: 138378 Human
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Alternative names
- Apoptotic cysteine protease Mih1/TX antibody
- CASP-4 antibody
- CASP4 antibody
see all
Images
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Western blot - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)All lanes : Anti-Caspase-4 antibody [EPR20921-83] (ab238124) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
Lane 2 : KARPAS-299 (human anaplastic large cell lymphoma), whole cell lysate
Lane 3 : IM-9 (human multiple myeloma B Lymphoblast), whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate
Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 32,38,45 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsNegative control: HEK-293T and Jurkat (PMID: 25119034).
The molecular weight observed is consistent with the literatures (PMID: 22246630, PMID: 25119034, PMID: 24879791, PMID: 15123740).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry (Intracellular) - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)](https://www.abcam.com/ps/products/238/ab238124/Images/ab238124-2-anti-caspase-4-antibody-epr20921-83-flowcytometry-thp1-293t-human.jpg)
Flow Cytometry (Intracellular) - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)Intracellular flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) cellslabeling Caspase-4 with ab238124 at 1/60 (right panel, red) compared with HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells (left panel, red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Negative control: HEK-293T (PMID: 25119034).
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![Flow Cytometry (Intracellular) - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)](https://www.abcam.com/ps/products/238/ab238124/Images/ab238124-3-anti-caspase-4-antibody-epr20921-83-flowcytometry-im9-human.jpg)
Flow Cytometry (Intracellular) - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized IM-9 (human multiple myeloma B Lymphoblast) cell line labeling Caspase 4with ab238124 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody.
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![Anti-Caspase-4 antibody [EPR20921-83] (ab238124)](https://www.abcam.com/ps/products/238/ab238124/Images/ab238124-4-benefits-of-recombinant-antibodies.png)
Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab238124 has been referenced in 1 publication.
- Zhou Y et al. lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR-500a-3p/XBP-1 axis. Mol Med Rep 24:N/A (2021). PubMed: 33982777
