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    caspase-4-antibody-epr20921-83-ab238124.pdf

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Cell Biology Apoptosis Intracellular Caspases etc Caspases
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RecombinantRabMAb

Recombinant Anti-Caspase-4 antibody [EPR20921-83] (ab238124)

  • Datasheet
  • Certificate of Compliance
Reviews (1) Submit a question References (1)

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Western blot - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
  • Flow Cytometry (Intracellular) - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
  • Flow Cytometry (Intracellular) - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
  • Anti-Caspase-4 antibody [EPR20921-83] (ab238124)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20921-83] to Caspase-4
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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View more associated products

Overview

  • Product name

    Anti-Caspase-4 antibody [EPR20921-83]
    See all Caspase-4 primary antibodies
  • Description

    Rabbit monoclonal [EPR20921-83] to Caspase-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt (Intra)more details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1, IM-9 and KARPAS-299 whole cell lysate. Flow Cyt (intra): THP-1 and IM-9 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20921-83
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-Caspase-4 antibody [EPR20921-83] - BSA and Azide free (ab256303)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Caspase-4 protein (Active) (ab51994)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab238124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000. Predicted molecular weight: 43 kDa.
Flow Cyt (Intra)
1/60.
Notes
WB
1/1000. Predicted molecular weight: 43 kDa.
Flow Cyt (Intra)
1/60.
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1.
  • Tissue specificity

    Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications

    The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8.
  • Target information above from: UniProt accession P49662 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 837 Human
    • Omim: 602664 Human
    • SwissProt: P49662 Human
    • Unigene: 138378 Human
    • Alternative names

      • Apoptotic cysteine protease Mih1/TX antibody
      • CASP-4 antibody
      • CASP4 antibody
      • CASP4_HUMAN antibody
      • Caspase 4 apoptosis related cysteine peptidase antibody
      • Caspase-4 subunit 2 antibody
      • Caspase4 antibody
      • ICE(rel)-II antibody
      • ICE(rel)II antibody
      • ICEREL II antibody
      • ICH2 antibody
      • Mih1/TX antibody
      • Protease ICH-2 antibody
      • Protease TX antibody
      • TX antibody
      see all

    Images

    • Western blot - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
      Western blot - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
      All lanes : Anti-Caspase-4 antibody [EPR20921-83] (ab238124) at 1/1000 dilution

      Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
      Lane 2 : KARPAS-299 (human anaplastic large cell lymphoma), whole cell lysate
      Lane 3 : IM-9 (human multiple myeloma B Lymphoblast), whole cell lysate
      Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate
      Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 43 kDa
      Observed band size: 32,38,45 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      Negative control: HEK-293T and Jurkat (PMID: 25119034). 

      The molecular weight observed is consistent with the literatures (PMID: 22246630, PMID: 25119034, PMID: 24879791, PMID: 15123740).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
      Flow Cytometry (Intracellular) - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)

      Intracellular flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) cellslabeling Caspase-4 with ab238124 at 1/60 (right panel, red) compared with HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells (left panel, red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Negative control: HEK-293T (PMID: 25119034). 

       

       

    • Flow Cytometry (Intracellular) - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
      Flow Cytometry (Intracellular) - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized IM-9 (human multiple myeloma B Lymphoblast) cell line labeling Caspase 4with ab238124 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody. 

       

       

    • Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
      Anti-Caspase-4 antibody [EPR20921-83] (ab238124)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab238124? Please let us know so that we can cite the reference in this datasheet.

    ab238124 has been referenced in 1 publication.

    • Zhou Y  et al. lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR-500a-3p/XBP-1 axis. Mol Med Rep 24:N/A (2021). PubMed: 33982777

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Caspase-4 antibody [EPR20921-83]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (LS174tT)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    1 µg
    Specification
    LS174tT
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 25 2022

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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