Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20921-83] to Caspase-4
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Product nameAnti-Caspase-4 antibody [EPR20921-83]
See all Caspase-4 primary antibodies
DescriptionRabbit monoclonal [EPR20921-83] to Caspase-4
Tested applicationsSuitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
- WB: THP-1, IM-9 and KARPAS-299 whole cell lysate. Flow Cyt (intra): THP-1 and IM-9 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 43 kDa.
|Flow Cyt (Intra)||
1/1000. Predicted molecular weight: 43 kDa.
Flow Cyt (Intra)
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1.
Tissue specificityWidely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8.
- Information by UniProt
- Apoptotic cysteine protease Mih1/TX antibody
- CASP-4 antibody
- CASP4 antibody
All lanes : Anti-Caspase-4 antibody [EPR20921-83] (ab238124) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
Lane 2 : KARPAS-299 (human anaplastic large cell lymphoma), whole cell lysate
Lane 3 : IM-9 (human multiple myeloma B Lymphoblast), whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate
Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 32,38,45 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
Negative control: HEK-293T and Jurkat (PMID: 25119034).
The molecular weight observed is consistent with the literatures (PMID: 22246630, PMID: 25119034, PMID: 24879791, PMID: 15123740).
Blocking/Dilution buffer: 5% NFDM/TBST.
Intracellular flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) cellslabeling Caspase-4 with ab238124 at 1/60 (right panel, red) compared with HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells (left panel, red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Negative control: HEK-293T (PMID: 25119034).
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized IM-9 (human multiple myeloma B Lymphoblast) cell line labeling Caspase 4with ab238124 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238124 has been referenced in 1 publication.
- Zhou Y et al. lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR-500a-3p/XBP-1 axis. Mol Med Rep 24:N/A (2021). PubMed: 33982777