CD38 Activity Assay Kit (Fluorometric) (ab284540)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
CD38 Activity Assay Kit (Fluorometric)
See all CD38 kits -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity -
Assay duration
Multiple steps standard assay -
Product overview
This CD38 Activity Assay Kit (Fluorometric) ab284540 (previously known as K2042) can be used to detect its activity in biological samples. The kit utilizes the activity of CD38 to catalyze the conversion of a CD38 substrate to a fluorescent product (Ex/Em = 300/410 nm).
This assay kit provides a rapid, simple and sensitive method to detect CD38 activity in a variety of sample types.
General range: 0 - 200 pmol/well
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K2042 CD38 Activity Assay Kit (Fluorometric). K2042-100 is the same size as the 100 test size of ab284540.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests CD38 Assay Buffer 1 x 25ml CD38 Lysis Buffer 1 x 25ml CD38 Positive Control 1 vial CD38 Standard 1 x 20µl CD38 (Cyclase) Substrate 1 vial -
Research areas
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Function
Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. -
Tissue specificity
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. -
Sequence similarities
Belongs to the ADP-ribosyl cyclase family. -
Developmental stage
Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Acute lymphoblastic leukemia cells antigen CD38
- ADP ribosyl cyclase
- ADP ribosyl cyclase 1
see all
Images
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CD38 Standard Curve. Assays were performed following the kit protocol.
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Reaction Kinetic curve of CD38 Positive Control, spleen lysate and B-cell lysate. Assays were performed following the kit protocol.
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CD38 activity assayed in rat spleen lysate (2 μg) and B-cell lysate (2.7 μg). Assays were performed following the kit protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284540 has not yet been referenced specifically in any publications.