Recombinant Anti-CD38 antibody [EPR21079] (ab216343)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)](https://www.abcam.com/ps/products/216/ab216343/Images/ab216343-297296-anti-cd38-antibody-epr21079-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21079] to CD38
- Suitable for: WB, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
Overview
-
Product name
Anti-CD38 antibody [EPR21079]
See all CD38 primary antibodies -
Description
Rabbit monoclonal [EPR21079] to CD38 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: P388D1 whole cell lysate; Mouse spleen, lymph node, colon and thymus lysates. IHC-P: Mouse spleen, liver and kidney tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21079 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).
|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa). |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. -
Tissue specificity
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. -
Sequence similarities
Belongs to the ADP-ribosyl cyclase family. -
Developmental stage
Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 12494 Mouse
- SwissProt: P56528 Mouse
- Unigene: 249873 Mouse
-
Form
There are 2 isoforms produced by alternative splicing. -
Alternative names
- Acute lymphoblastic leukemia cells antigen CD38 antibody
- ADP ribosyl cyclase 1 antibody
- ADP ribosyl cyclase antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in endothelial cells of mouse kidney (PMID: 25078456). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)](https://www.abcam.com/ps/products/216/ab216343/Images/ab216343-297295-anti-cd38-antibody-epr21079-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in the sinusoids of mouse liver (PMID: 17200192). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)](https://www.abcam.com/ps/products/216/ab216343/Images/ab216343-297294-anti-cd38-antibody-epr21079-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse spleen (PMID: 10360965). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-CD38 antibody [EPR21079] (ab216343)](https://www.abcam.com/ps/products/216/ab216343/Images/ab216343-297293-anti-cd38-antibody-epr21079-western-blot.jpg)
Western blot - Anti-CD38 antibody [EPR21079] (ab216343)All lanes : Anti-CD38 antibody [EPR21079] (ab216343) at 1/1000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : P388D1 (mouse lymphoma monocyte; macrophage cell line) whole cell lysate
Lane 3 : Mouse lymph node lysate
Lane 4 : Mouse colon lysate
Lane 5 : Mouse thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument. The observed molecular mass is consistent with the literature (PMID: 7774637).
-
![Anti-CD38 antibody [EPR21079] (ab216343)](https://www.abcam.com/ps/products/216/ab216343/Images/ab216343-5-benefits-of-recombinant-antibodies.png)
Anti-CD38 antibody [EPR21079] (ab216343)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (2)
ab216343 has been referenced in 2 publications.
- Kim HN et al. A decrease in NAD+ contributes to the loss of osteoprogenitors and bone mass with aging. NPJ Aging Mech Dis 7:8 (2021). PubMed: 33795658
- An Z et al. EGFR Cooperates with EGFRvIII to Recruit Macrophages in Glioblastoma. Cancer Res 78:6785-6794 (2018). PubMed: 30401716
