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    cleaved-n-terminal-dfna5--gsdme-antibody-epr20866-160-ab222407.pdf

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RecombinantRabMAb

Recombinant Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (3)

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Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)
  • Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20866-160] to cleaved N-terminal DFNA5 / GSDME
  • Suitable for: WB
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Human DFNA5/GSDME protein (ab116824)
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Primary
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Anti-GSDMD antibody [EPR19828] (ab209845)

View more associated products

Overview

  • Product name

    Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160]
    See all cleaved N-terminal DFNA5 / GSDME primary antibodies
  • Description

    Rabbit monoclonal [EPR20866-160] to cleaved N-terminal DFNA5 / GSDME
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: CT26 (treated with 2 µg/ml Actinomycin-D for 24 hours) whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20866-160
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Sensory System
    • Auditory system

Associated products

  • Alternative Versions

    • Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] - BSA and Azide free (ab256122)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Related Products

    • Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab222407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 57 kDa).
Notes
WB
1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 57 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53 (PubMed:16897187, PubMed:18223688, PubMed:21522185). The N-terminal moiety promotes pyroptosis and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown.
  • Tissue specificity

    Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.
  • Involvement in disease

    Deafness, autosomal dominant, 5
    Is a tumor suppressor gene with an important role in colorectal cancer (CRC).
  • Sequence similarities

    Belongs to the gasdermin family.
  • Domain

    Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain.
  • Cellular localization

    Cytoplasm, cytosol. Cell membrane.
  • Target information above from: UniProt accession O60443 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • DFNA5 antibody
    • DFNA5_HUMAN antibody
    • ICERE-1 antibody
    • Inversely correlated with estrogen receptor expression 1 antibody
    • Non-syndromic hearing impairment protein 5 antibody
    see all

Images

  • Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)
    Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)
    All lanes : Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407) at 1/5000 dilution

    Lane 1 : Untreated CT26 (mouse colon carcinoma) whole cell lysate
    Lane 2 : CT26 (treated with 2 µg/ml Actinomycin-D for 24 hours) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 57 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?


    Exposure time: 103 seconds


    Lysates were kindly provided by Dr Feng Shao.

    The molecular weight observed is consistent with the literature (PMID: 28459430, 30804337).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)
    Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (3)

Publishing research using ab222407? Please let us know so that we can cite the reference in this datasheet.

ab222407 has been referenced in 3 publications.

  • Chen L  et al. JQ1 as a BRD4 Inhibitor Blocks Inflammatory Pyroptosis-Related Acute Colon Injury Induced by LPS. Front Immunol 12:609319 (2021). PubMed: 33679744
  • Tang J  et al. Acute cadmium exposure induces GSDME-mediated pyroptosis in triple-negative breast cancer cells through ROS generation and NLRP3 inflammasome pathway activation. Environ Toxicol Pharmacol 87:103686 (2021). PubMed: 34098069
  • Liu Y  et al. Methamphetamine induces GSDME-dependent cell death in hippocampal neuronal cells through the endoplasmic reticulum stress pathway. Brain Res Bull 162:73-83 (2020). PubMed: 32544512

Customer reviews and Q&As

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