Recombinant Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)
![Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)](https://www.abcam.com/ps/products/222/ab222407/Images/ab222407-1-anti-cleaved-n-terminal-dfna5-gsdme-antibody-epr20866-160-western-blot-ct26-mouse.jpg "Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20866-160] to cleaved N-terminal DFNA5 / GSDME
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160]
See all cleaved N-terminal DFNA5 / GSDME primary antibodies -
Description
Rabbit monoclonal [EPR20866-160] to cleaved N-terminal DFNA5 / GSDME -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: CT26 (treated with 2 µg/ml Actinomycin-D for 24 hours) whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20866-160 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 57 kDa).
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| Notes |
|---|
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WB
1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 57 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53 (PubMed:16897187, PubMed:18223688, PubMed:21522185). The N-terminal moiety promotes pyroptosis and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown. -
Tissue specificity
Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta. -
Involvement in disease
Deafness, autosomal dominant, 5
Is a tumor suppressor gene with an important role in colorectal cancer (CRC). -
Sequence similarities
Belongs to the gasdermin family. -
Domain
Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain. -
Cellular localization
Cytoplasm, cytosol. Cell membrane. - Information by UniProt
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Alternative names
- DFNA5 antibody
- DFNA5_HUMAN antibody
- ICERE-1 antibody
see all
Images
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Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)All lanes : Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407) at 1/5000 dilution
Lane 1 : Untreated CT26 (mouse colon carcinoma) whole cell lysate
Lane 2 : CT26 (treated with 2 µg/ml Actinomycin-D for 24 hours) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 57 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 103 secondsLysates were kindly provided by Dr Feng Shao.
The molecular weight observed is consistent with the literature (PMID: 28459430, 30804337).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)](https://www.abcam.com/ps/products/222/ab222407/Images/ab222407-2-benefits-of-recombinant-antibodies.png)
Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (3)
ab222407 has been referenced in 3 publications.
- Chen L et al. JQ1 as a BRD4 Inhibitor Blocks Inflammatory Pyroptosis-Related Acute Colon Injury Induced by LPS. Front Immunol 12:609319 (2021). PubMed: 33679744
- Tang J et al. Acute cadmium exposure induces GSDME-mediated pyroptosis in triple-negative breast cancer cells through ROS generation and NLRP3 inflammasome pathway activation. Environ Toxicol Pharmacol 87:103686 (2021). PubMed: 34098069
- Liu Y et al. Methamphetamine induces GSDME-dependent cell death in hippocampal neuronal cells through the endoplasmic reticulum stress pathway. Brain Res Bull 162:73-83 (2020). PubMed: 32544512
