Name: Anti-DDX4 / MVH antibody [EPR24148-58]
Brand: RabMAb
SKU: ab270534
Rating: 5 (1 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24148-58] to DDX4 / MVH
Suitable for: IHC-Fr, IHC-P, WB
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-DDX4 / MVH antibody [EPR24148-58]
See all DDX4 / MVH primary antibodies
Description

Rabbit monoclonal [EPR24148-58] to DDX4 / MVH
Host species

Rabbit
Tested applications

Suitable for: IHC-Fr, IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human testis tissue lysate; Mouse testis tissue lysate; rat testis tissue lysate. IHC-P: Human testis and overy tissue; Mouse testis and ovary tissue; Rat testis and ovary tissue. IHC-Fr: Mouse testis tissue; Rat testis tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24148-58
Isotype

IgG
Research areas

Alternative Versions
- Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
Compatible Secondaries

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-Fr	(1)	1/250. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).

Notes
IHC-Fr 1/250. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).

Application notes

Is unsuitable for Flow Cyt,ICC or IP.

Function

Probable ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle.
Tissue specificity

Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
Sequence similarities

Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localization

Cytoplasm. Cytoplasm, perinuclear region. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
Target information above from: UniProt accession Q9NQI0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 54514 Human
- Entrez Gene: 13206 Mouse
- Entrez Gene: 310090 Rat
- Omim: 605281 Human
- SwissProt: Q9NQI0 Human
- SwissProt: Q61496 Mouse
- SwissProt: Q64060 Rat
- Unigene: 223581 Human
- Unigene: 12818 Mouse
- Unigene: 198577 Rat
see all
Alternative names
- DDX 4 antibody
- Ddx4 antibody
- DDX4_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody
- DEAD box protein 4 antibody
- DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody
- MVH antibody
- Probable ATP dependent RNA helicase DDX4 antibody
- Probable ATP-dependent RNA helicase DDX4 antibody
- VASA antibody
- Vasa homolog antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in spermatogonia and spermatocytes of human testis (PMID:26444630). The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling DDX4 / MVH with ab270534 at 1/250 (1.824 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse testis is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)

All lanes : Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534) at 1/1000 dilution

Lane 1 : Human testis tissue lysate
Lane 2 : Human spleen tissue lysate
Lane 3 : Human colon tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 79 kDa
Observed band size: 79 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: Human spleen, Human colon (PMID: 10920202).

Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in spermatogonia and spermatocytes of mouse testis. The section was incubated with ab270534 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat testis tissue labeling DDX4 / MVH with ab270534 at 1/250 (1.824 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat testis is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)

Immunohistochemical analysis of paraffin-embedded Human ovary tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in oocytes of human ovary (PMID:26444630).The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)

All lanes : Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534) at 1/1000 dilution

Lane 1 : Rat testis tissue lysate
Lane 2 : Mouse testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 79 kDa
Observed band size: 79 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure times: lane 1: 10 seconds lane 2: 7 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)

Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in oocytes of mouse ovary. The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in spermatogonia and spermatocytes of rat testis. The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)

Immunohistochemical analysis of paraffin-embedded Rat ovary tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in oocytes of rat ovary. The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab270534? Please let us know so that we can cite the reference in this datasheet.

ab270534 has been referenced in 1 publication.

Li J et al. Quercetin prevents primordial follicle loss via suppression of PI3K/Akt/Foxo3a pathway activation in cyclophosphamide-treated mice. Reprod Biol Endocrinol 19:63 (2021). PubMed: 33892727

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Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (E14.5 mouse embryonic ovary)

Permeabilization

Specification

E14.5 mouse embryonic ovary

Blocking step

Heat inactivated donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Paraformaldehyde

Steven Cincotta

Verified customer

Submitted Feb 06 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (1)

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-DDX4 / MVH antibody [EPR24148-58]