Recombinant Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)
![Western blot - Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)](https://www.abcam.com/ps/products/156/ab156006/Images/ab156006-163343-anti-dkc1-antibody-epr10398-western-blot.jpg "Western blot - Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10398] to DKC1/Dyskerin
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DKC1/Dyskerin antibody [EPR10398]
See all DKC1/Dyskerin primary antibodies -
Description
Rabbit monoclonal [EPR10398] to DKC1/Dyskerin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SW480, MCF7, HeLa, and HepG2 whole cell lysate (ab7900), HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10398 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 58 kDa.
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 58 kDa. |
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ICC/IF
1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.
Target
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Function
Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells). -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Defects in DKC1 are a cause of dyskeratosis congenita X-linked recessive (XDKC) [MIM:305000]. XDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
Defects in DKC1 are the cause of Hoyeraal-Hreidarsson syndrome (HHS) [MIM:300240]. HHS is a multisystem disorder affecting males and is characterized by aplastic anemia, immunodeficiency, microcephaly, cerebellar hypoplasia, and growth retardation. -
Sequence similarities
Belongs to the pseudouridine synthase TruB family.
Contains 1 PUA domain. -
Cellular localization
Cytoplasm and Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies. - Information by UniProt
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Database links
- Entrez Gene: 1736 Human
- Entrez Gene: 245474 Mouse
- Entrez Gene: 170944 Rat
- Omim: 300126 Human
- SwissProt: O60832 Human
- SwissProt: Q9ESX5 Mouse
- SwissProt: P40615 Rat
- Unigene: 4747 Human
see all -
Alternative names
- CBF5 antibody
- CBF5 homolog antibody
- Cbf5p homolog antibody
see all
Images
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Western blot - Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)All lanes : Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Observed band size: 58 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)](https://www.abcam.com/ps/products/156/ab156006/Images/ab156006-163342-anti-dkc1-antibody-epr10398-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)Immunofluorescent analysis of HeLa cells labeling DKC1/Dyskerin with ab156006 at 1/100.
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![Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)](https://www.abcam.com/ps/products/156/ab156006/Images/ab156006-3-benefits-of-recombinant-antibodies.png)
Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab156006 has not yet been referenced specifically in any publications.