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  1. Link

    dkc1dyskerin-antibody-epr10398-ab156006.pdf

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Epigenetics and Nuclear Signaling Chromosome Structure Telomeres
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RecombinantRabMAb

Recombinant Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)

  • Datasheet
  • SDS
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Western blot - Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)
  • Immunocytochemistry/ Immunofluorescence - Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)
  • Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10398] to DKC1/Dyskerin
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-DKC1/Dyskerin antibody [EPR10398]
    See all DKC1/Dyskerin primary antibodies
  • Description

    Rabbit monoclonal [EPR10398] to DKC1/Dyskerin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SW480, MCF7, HeLa, and HepG2 whole cell lysate (ab7900), HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10398
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Telomeres
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Alternative Versions

    • Anti-DKC1/Dyskerin antibody [EPR10398] - BSA and Azide free (ab238928)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
    • SW480 whole cell lysate (ab3957)
  • Recombinant Protein

    • Recombinant Human DKC1/Dyskerin protein (ab152335)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab156006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 58 kDa.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 58 kDa.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.

Target

  • Function

    Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
    Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells).
  • Tissue specificity

    Ubiquitously expressed.
  • Involvement in disease

    Defects in DKC1 are a cause of dyskeratosis congenita X-linked recessive (XDKC) [MIM:305000]. XDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
    Defects in DKC1 are the cause of Hoyeraal-Hreidarsson syndrome (HHS) [MIM:300240]. HHS is a multisystem disorder affecting males and is characterized by aplastic anemia, immunodeficiency, microcephaly, cerebellar hypoplasia, and growth retardation.
  • Sequence similarities

    Belongs to the pseudouridine synthase TruB family.
    Contains 1 PUA domain.
  • Cellular localization

    Cytoplasm and Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies.
  • Target information above from: UniProt accession O60832 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1736 Human
    • Entrez Gene: 245474 Mouse
    • Entrez Gene: 170944 Rat
    • Omim: 300126 Human
    • SwissProt: O60832 Human
    • SwissProt: Q9ESX5 Mouse
    • SwissProt: P40615 Rat
    • Unigene: 4747 Human
    • Unigene: 291062 Mouse
    • Unigene: 4223 Rat
    see all
  • Alternative names

    • CBF5 antibody
    • CBF5 homolog antibody
    • Cbf5p homolog antibody
    • DKC 1 antibody
    • DKC antibody
    • Dkc1 antibody
    • DKC1_HUMAN antibody
    • DKCX antibody
    • Dyskeratosis congenita 1 antibody
    • Dyskeratosis congenita 1 dyskerin antibody
    • Dyskerin antibody
    • H/ACA ribonucleoprotein complex subunit 4 antibody
    • NAP 57 antibody
    • NAP57 antibody
    • NOLA 4 antibody
    • NOLA4 antibody
    • Nopp140 associated protein of 57 kDa antibody
    • Nopp140-associated protein of 57 kDa antibody
    • Nucleolar protein family A member 4 antibody
    • Nucleolar protein NAP57 antibody
    • snoRNP protein DKC1 antibody
    • XAP 101 antibody
    • XAP101 antibody
    see all

Images

  • Western blot - Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)
    Western blot - Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)
    All lanes : Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006) at 1/1000 dilution

    Lane 1 : SW480 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Observed band size: 58 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)
    Immunocytochemistry/ Immunofluorescence - Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)

    Immunofluorescent analysis of HeLa cells labeling DKC1/Dyskerin with ab156006 at 1/100.

  • Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)
    Anti-DKC1/Dyskerin antibody [EPR10398] (ab156006)

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab156006? Please let us know so that we can cite the reference in this datasheet.

ab156006 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Western blot abreview for Anti-DKC1/Dyskerin antibody [EPR10398]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HES2 human embryonic stem cells, 293T, HeLa)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
HES2 human embryonic stem cells, 293T, HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Jianlong Wang

Verified customer

Submitted Jan 03 2018

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