Recombinant Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
![Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-384461-anti-dna-polymerase-beta-antibody-epr12722b-western-blot-hela-jurkat.jpg "Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta
- Suitable for: ICC/IF, IP, IHC-P, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DNA Polymerase beta antibody [EPR12722(B)]
See all DNA Polymerase beta primary antibodies -
Description
Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat, K562 and A431 cell lysates. IHC-P: Human testis and uterus tissues. ICC/IF: A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12722(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
1/50 - 1/100.
|
| IP |
1/10 - 1/100.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
|
| Notes |
|---|
|
ICC/IF
1/50 - 1/100. |
|
IP
1/10 - 1/100. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
Target
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Function
Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. -
Sequence similarities
Belongs to the DNA polymerase type-X family. -
Domain
Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity. -
Post-translational
modificationsMethylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. -
Cellular localization
Nucleus, Cytoplasm - Information by UniProt
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Database links
- Entrez Gene: 5423 Human
- Entrez Gene: 18970 Mouse
- Entrez Gene: 29240 Rat
- Omim: 174760 Human
- SwissProt: P06746 Human
- SwissProt: Q8K409 Mouse
- SwissProt: P06766 Rat
- Unigene: 654484 Human
see all -
Alternative names
- DNA directed DNA polymerase beta antibody
- DNA pol beta antibody
- DNA polymerase beta antibody
see all
Images
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Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)All lanes : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : POLB knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDaanes 1-3: Merged signal (red and green). Green - ab175197 observed at 38 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab175197 Anti-DNA Polymerase beta antibody [EPR12722(B)] was shown to specifically react with DNA Polymerase beta in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261748 (knockout cell lysate ab257096) was used. Wild-type and DNA Polymerase beta knockout samples were subjected to SDS-PAGE. ab175197 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-201547-anti-dna-polymerase-beta-antibody-epr12722b-western-blot.jpg)
Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)All lanes : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : K562 cell lysates
Lane 4 : A431 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-201546-anti-dna-polymerase-beta-antibody-epr12722b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-201545-anti-dna-polymerase-beta-antibody-epr12722b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-201544-anti-dna-polymerase-beta-antibody-epr12722b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)Immunofluorescence analysis of A431 cells labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.
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![Immunoprecipitation - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-201543-anti-dna-polymerase-beta-antibody-epr12722b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)Western blot analysis on immunoprecipitation pellet from K562 cell lysate labeling DNA Polymerase beta with ab175197 at 1/10 dilution.
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![Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-7-benefits-of-recombinant-antibodies.png)
Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab175197 has been referenced in 4 publications.
- Jiang Z et al. Oxidative DNA Damage Modulates DNA Methylation Pattern in Human Breast Cancer 1 (BRCA1) Gene via the Crosstalk between DNA Polymerase ß and a de novo DNA Methyltransferase. Cells 9:N/A (2020). PubMed: 31963223
- Goetze RW et al. A CRISPR/Cas9 approach reveals that the polymerase activity of DNA polymerase ß is dispensable for HIV-1 infection in dividing and nondividing cells. J Biol Chem 292:14016-14025 (2017). PubMed: 28684413
- Lai Y et al. Crosstalk between MSH2-MSH3 and polß promotes trinucleotide repeat expansion during base excision repair. Nat Commun 7:12465 (2016). PubMed: 27546332
- Wang H et al. Cell cycle regulation of DNA polymerase beta in rotenone-based Parkinson's disease models. PLoS One 9:e109697 (2014). Human . PubMed: 25303312
