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  1. Link

    dna-polymerase-beta-antibody-epr12722b-ab175197.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Base Excision Repair
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RecombinantRabMAb

Recombinant Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

  • Datasheet
Reviews (4) Submit a question References (5)

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Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
  • Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
  • Flow Cytometry - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
  • Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Human DNA Polymerase beta protein (ab111641)
Primary
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Anti-LIG1 antibody [EPR12464] (ab177946)

View more associated products

Overview

  • Product name

    Anti-DNA Polymerase beta antibody [EPR12722(B)]
    See all DNA Polymerase beta primary antibodies
  • Description

    Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta
  • Host species

    Rabbit
  • Specificity

    The human recommendation is based on the WB results. We do not guarantee IHC-P for human

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat, K-562, A431, Mouse brain lysate, mouse spleen lysate, rat brain lysate, rat spleen lysate, C6 cell lysate ICC/IF: K-562 (Human chronic myelogenous leukemia lymphoblast) IHC: human lung carcinoma
  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12722(B)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Base Excision Repair
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • DNA Polymerases
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free (ab249852)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human DNA Polymerase beta protein (ab111641)
  • Related Products

    • Recombinant Human DNA Polymerase beta protein (ab111641)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/50.
Flow Cyt
1/200.
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The human recommendation is based on the WB results. We do not guarantee IHC-P for human

WB (3)
1/1000. Predicted molecular weight: 38 kDa.
Notes
ICC/IF
1/50.
Flow Cyt
1/200.
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The human recommendation is based on the WB results. We do not guarantee IHC-P for human

WB
1/1000. Predicted molecular weight: 38 kDa.
Application notes
Is unsuitable for IP.

Target

  • Function

    Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
  • Sequence similarities

    Belongs to the DNA polymerase type-X family.
  • Domain

    Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
  • Post-translational
    modifications

    Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
  • Cellular localization

    Nucleus, Cytoplasm
  • Target information above from: UniProt accession P06746 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5423 Human
    • Entrez Gene: 18970 Mouse
    • Entrez Gene: 29240 Rat
    • Omim: 174760 Human
    • SwissProt: P06746 Human
    • SwissProt: Q8K409 Mouse
    • SwissProt: P06766 Rat
    • Unigene: 654484 Human
    • Unigene: 123211 Mouse
    • Unigene: 473777 Mouse
    • Unigene: 9346 Rat
    see all
  • Alternative names

    • DNA directed DNA polymerase beta antibody
    • DNA pol beta antibody
    • DNA polymerase beta antibody
    • DNA polymerase beta subunit antibody
    • DPOLB_HUMAN antibody
    • MGC125976 antibody
    • Pol B antibody
    • Pol beta antibody
    • POLB antibody
    • Polymerase (DNA directed) beta antibody
    see all

Images

  • Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    Lanes 1-7 : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/1000 dilution (Purified)
    Lanes 8-9 : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 3 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 4 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 5 : Mouse brain lysate
    Lane 6 : Mouse spleen lysate
    Lane 7 : Rat brain lysate
    Lane 8 : Rat spleen lysate
    Lane 9 : C6 (Rat glial tumor glial cell) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

    Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling DNA Polymerase beta with Purified ab175197 at 1:50 dilution (3.1 ?g/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling with Purified ab175197 at 1:500 dilution (0.31 ?g/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. M3

  • Flow Cytometry - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    Flow Cytometry - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

    Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labelling DNA Polymerase beta with Purified ab175197 at 1:200 dilution (1 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (5)

Publishing research using ab175197? Please let us know so that we can cite the reference in this datasheet.

ab175197 has been referenced in 5 publications.

  • Wandt VK  et al. Ageing-associated effects of a long-term dietary modulation of four trace elements in mice. Redox Biol 46:102083 (2021). PubMed: 34371368
  • Jiang Z  et al. Oxidative DNA Damage Modulates DNA Methylation Pattern in Human Breast Cancer 1 (BRCA1) Gene via the Crosstalk between DNA Polymerase ß and a de novo DNA Methyltransferase. Cells 9:N/A (2020). PubMed: 31963223
  • Goetze RW  et al. A CRISPR/Cas9 approach reveals that the polymerase activity of DNA polymerase ß is dispensable for HIV-1 infection in dividing and nondividing cells. J Biol Chem 292:14016-14025 (2017). PubMed: 28684413
  • Lai Y  et al. Crosstalk between MSH2-MSH3 and polß promotes trinucleotide repeat expansion during base excision repair. Nat Commun 7:12465 (2016). PubMed: 27546332
  • Wang H  et al. Cell cycle regulation of DNA polymerase beta in rotenone-based Parkinson's disease models. PLoS One 9:e109697 (2014). Human . PubMed: 25303312

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-DNA Polymerase beta antibody [EPR12722(B)]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cell and PolB knockout cell lysates)
Gel Running Conditions
Reduced Denaturing (4-12% BIS-TRIS gradient, MOPS running buffer)
Loading amount
20 µg
Specification
Hela cell and PolB knockout cell lysates
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Nadeem Shaikh

Verified customer

Submitted May 20 2021

Western blot abreview for Anti-DNA Polymerase beta antibody [EPR12722(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Osteosarcoma Cells)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
30 µg
Specification
Osteosarcoma Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

MR. Md Ibrahim

Verified customer

Submitted Jul 23 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-DNA Polymerase beta antibody [EPR12722(B)]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (spermatocytes)
Permeabilization
No
Specification
spermatocytes
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Prasada Rao

Verified customer

Submitted Jun 27 2016

Western blot abreview for Anti-DNA Polymerase beta antibody [EPR12722(B)]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Spermatocytes)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
30 µg
Specification
Spermatocytes
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jun 27 2016

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