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  1. Link

    eif4a3-antibody-epr14301b-ab180573.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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RecombinantRabMAb

Recombinant Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (4) Submit a question References (6)

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Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
  • Flow Cytometry (Intracellular) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
  • Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
  • Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14301(B)] to eIF4A3
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

You may also be interested in

Protein
Product image
Recombinant Human eIF4A3 protein (ab123151)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
Product image
Anti-IFNGR1 antibody [EPR7866] - BSA and Azide free (ab226151)

View more associated products

Overview

  • Product name

    Anti-eIF4A3 antibody [EPR14301(B)]
    See all eIF4A3 primary antibodies
  • Description

    Rabbit monoclonal [EPR14301(B)] to eIF4A3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MCF7, HeLa, Raji and HepG2 whole cell lysate (ab7900); MCF7 and HeLa cells; Human prostate tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14301(B)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-eIF4A3 antibody [EPR14301(B)] (ab225279)
    • Alexa Fluor® 488 Anti-eIF4A3 antibody [EPR14301(B)] (ab225280)
    • PE Anti-eIF4A3 antibody [EPR14301(B)] (ab225281)
    • Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
  • Recombinant Protein

    • Recombinant Human eIF4A3 protein (ab123151)
  • Related Products

    • Recombinant Human eIF4A3 protein (ab123151)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (1)
1/1000 - 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF (2)
1/500.
IP (1)
1/30.
Notes
Flow Cyt (Intra)
1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
IP
1/30.

Target

  • Function

    ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the DEAD box helicase family. eIF4A subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites.
  • Target information above from: UniProt accession P38919 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 416704 Chicken
    • Entrez Gene: 515145 Cow
    • Entrez Gene: 101867456 Cynomolgus monkey
    • Entrez Gene: 9775 Human
    • Entrez Gene: 192170 Mouse
    • Entrez Gene: 100101926 Pig
    • Entrez Gene: 688288 Rat
    • Entrez Gene: 399362 Xenopus laevis
    • Entrez Gene: 100135173 Xenopus tropicalis
    • Entrez Gene: 394053 Zebrafish
    • Omim: 608546 Human
    • SwissProt: Q5ZM36 Chicken
    • SwissProt: Q2NL22 Cow
    • SwissProt: Q4R3Q1 Cynomolgus monkey
    • SwissProt: P38919 Human
    • SwissProt: Q91VC3 Mouse
    • SwissProt: A6M931 Pig
    • SwissProt: Q3B8Q2 Rat
    • SwissProt: O42226 Xenopus laevis
    • SwissProt: B7ZTW1 Xenopus tropicalis
    • SwissProt: Q7ZVA6 Zebrafish
    • Unigene: 389649 Human
    • Unigene: 197555 Mouse
    • Unigene: 422975 Mouse
    • Unigene: 202617 Rat
    • Unigene: 78203 Zebrafish
    see all
  • Alternative names

    • ATP-dependent RNA helicase DDX48 antibody
    • ATP-dependent RNA helicase eIF4A-3 antibody
    • DDX48 antibody
    • DEAD box protein 48 antibody
    • eIF-4A-III antibody
    • eIF4A-III antibody
    • EIF4A3 antibody
    • eIF4AIII antibody
    • Eukaryotic initiation factor 4A-III antibody
    • Eukaryotic initiation factor 4A-like NUK-34 antibody
    • Eukaryotic translation initiation factor 4A isoform 3 antibody
    • hNMP 265 antibody
    • IF4A3_HUMAN antibody
    • NMP 265 antibody
    • NMP265 antibody
    • Nuclear matrix protein 265 antibody
    • NUK34 antibody
    see all

Images

  • Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    All lanes : Anti-eIF4A3 antibody [EPR14301(B)] (ab180573) at 1/1000 dilution

    Lane 1 : MCF7 cell line with NFDM
    Lane 2 : Hela cell line with NFDM
    Lane 3 : Raji cell line with NFDM
    Lane 4 : HepG2 cell line with NFDM

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Goat Anti-rabbit HRP at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling eIF4A3 with ab180573 at 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Immunofluorescent analysis of 4% paraformaldehyde MCF7 cells labeling eIF4A3 with ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/200.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Immunofluorescent analysis of 4% paraformaldehyde Hela cells labeling eIF4A3 with ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody at a dilution of 1/200.

  • Flow Cytometry (Intracellular) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Flow Cytometry (Intracellular) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Intracellular flow cytometric analysis of 2% paraformaldehydeMCF7 cells labeling eIF4A3 with ab180573 at 1/160 dilution,or a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution. 

     

     

  • Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Immunoprecipitation of Hela labeling eIF4A3 using ab180573 at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST.



    All lanes : Anti-eIF4A3 antibody [EPR14301(B)] (ab180573) at 1/30 dilution

    Lane 1 : Hela cell lysate with NFDM
    Lane 2 : Negative control with NFDM

    Blocking peptides at 5 % per lane.
  • Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (6)

Publishing research using ab180573? Please let us know so that we can cite the reference in this datasheet.

ab180573 has been referenced in 6 publications.

  • Zhu Y  et al. RPL34-AS1-induced RPL34 inhibits cervical cancer cell tumorigenesis via the MDM2-P53 pathway. Cancer Sci 112:1811-1821 (2021). PubMed: 33675124
  • Sui X  et al. hsa_circ_0101119 facilitates the progression of cervical cancer via an interaction with EIF4A3 to inhibit TCEAL6 expression. Mol Med Rep 24:N/A (2021). PubMed: 34278492
  • Shadle SC  et al. DUX4-induced bidirectional HSATII satellite repeat transcripts form intranuclear double-stranded RNA foci in human cell models of FSHD. Hum Mol Genet 28:3997-4011 (2019). PubMed: 31630170
  • Ren X  et al. Avian Influenza A Virus Polymerase Recruits Cellular RNA Helicase eIF4A3 to Promote Viral mRNA Splicing and Spliced mRNA Nuclear Export. Front Microbiol 10:1625 (2019). PubMed: 31379779
  • Viphakone N  et al. Co-transcriptional Loading of RNA Export Factors Shapes the Human Transcriptome. Mol Cell 75:310-323.e8 (2019). PubMed: 31104896
  • Shadle SC  et al. DUX4-induced dsRNA and MYC mRNA stabilization activate apoptotic pathways in human cell models of facioscapulohumeral dystrophy. PLoS Genet 13:e1006658 (2017). ICC/IF ; Human . PubMed: 28273136

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-eIF4A3 antibody [EPR14301(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Osteosarcoma)
Permeabilization
Yes - 0.3% tritonX-100
Specification
Osteosarcoma
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 29 2021

Immunoprecipitation abreview for Anti-eIF4A3 antibody [EPR14301(B)]

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (mouse ESC (CCE))
Total protein in input
6e+006 cells
Immuno-precipitation step
Protein G
Specification
mouse ESC (CCE)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 30 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-eIF4A3 antibody [EPR14301(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse ES cell line (CCE))
Permeabilization
Yes - 0.25% TritonX-100 solution (dilute in PBS)
Specification
mouse ES cell line (CCE)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 27 2017

Western blot abreview for Anti-eIF4A3 antibody [EPR14301(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse ESC (CCE))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Mouse ESC (CCE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 26 2017

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