Recombinant Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)
![Western blot - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)](https://www.abcam.com/ps/products/79/ab79413/Images/ab79413-298193-anti-estrogen-receptor-alpha-antibody-epr7032-western-blot.jpg "Western blot - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR703(2)] to Estrogen Receptor alpha
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Estrogen Receptor alpha antibody [EPR703(2)]
See all Estrogen Receptor alpha primary antibodies -
Description
Rabbit monoclonal [EPR703(2)] to Estrogen Receptor alpha -
Host species
Rabbit -
Specificity
Expression levels of ER alpha protein vary with sample type. -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Baboon
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF-7 cell lysate. Human breast carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR703(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 68 kDa).
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| IHC-P | (7) |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 68 kDa). |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
Cellular localization
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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Database links
- Entrez Gene: 2099 Human
- Omim: 133430 Human
- SwissProt: P03372 Human
- Unigene: 208124 Human
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Alternative names
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
Images
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Western blot - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)All lanes : Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413) at 1/2000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell). Whole cell lysates
Lane 2 : T-47D (human mammary gland ductal carcinoma epithelial cell). Whole cell lysates
Lane 3 : MDA-MB231 (Human breast adenocarcinoma epithelial cell) Whole cell lysates (Negative control)
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) Whole cell lysates (Negative control)
Lane 5 : Human uterus whole tissue lysate
Lane 6 : Human ovary whole tissue lysate
Lane 7 : Human ovary cancer whole tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)](https://www.abcam.com/ps/products/79/ab79413/Images/ab79413-94966-anti-estrogen-receptor-alpha-antibody-epr7032-western-blot.jpg)
Western blot - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413) at 1/1000 dilution + MCF-7 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 68 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)](https://www.abcam.com/ps/products/79/ab79413/Images/ab79413-94965-anti-estrogen-receptor-alpha-antibody-epr7032-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)ab79413, at 1/500 dilution, staining Estrogen Receptor alpha in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)](https://www.abcam.com/ps/products/79/ab79413/Images/ab79413-232139-anti-estrogen-receptor-alpha-antibody-epr7032-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)This image is courtesy of an Abreview submitted by Marcia Burchab79413 staining Estrogen Receptor alpha in Baboon placenta and fetal ovary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in 10mM sodium citrate, pH 6.0. Samples were incubated with primary antibody (1/500 in PBS + 5% normal goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (7µg/ml) was used as the secondary antibody.
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![Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)](https://www.abcam.com/ps/products/79/ab79413/Images/ab79413-5-benefits-of-recombinant-antibodies.png)
Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab79413 has been referenced in 4 publications.
- Han N et al. The G-Protein-Coupled Estrogen Receptor (GPER) Regulates Trimethylation of Histone H3 at Lysine 4 and Represses Migration and Proliferation of Ovarian Cancer Cells In Vitro. Cells 10:N/A (2021). PubMed: 33799631
- Liu Y et al. Efficacy and safety of TE/TEC/intensive paclitaxel neoadjuvant chemotherapy for the treatment of breast cancer. Oncol Lett 17:907-912 (2019). PubMed: 30655846
- Xin L et al. A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility. Acta Biomater 92:160-171 (2019). PubMed: 31075515
- Hirao-Suzuki M et al. Repeated Exposure to 4-Methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene (MBP), an Active Metabolite of Bisphenol A, Aggressively Stimulates Breast Cancer Cell Growth in an Estrogen Receptor ß (ERß)-Dependent Manner. Mol Pharmacol 95:260-268 (2019). PubMed: 30552153
