RecombinantRabMAb

Recombinant Anti-ETS2 antibody [EPR22419] (ab219948)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)
  • Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody [EPR22419] (ab219948)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)
  • Immunoprecipitation - Anti-ETS2 antibody [EPR22419] (ab219948)
  • Western blot - Anti-ETS2 antibody [EPR22419] (ab219948)
  • Western blot - Anti-ETS2 antibody [EPR22419] (ab219948)
  • Anti-ETS2 antibody [EPR22419] (ab219948)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22419] to ETS2
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-ETS2 antibody [EPR22419]
    See all ETS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22419] to ETS2

  • Host species

    Rabbit

  • Specificity

    This antibody is not recommended for human in IHC.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: LPS treated RAW 264.7 cell lysate; K562 whole cell lysate; Human thymus lysate. IHC-P: Mouse and rat thymus tissue. ICC/IF: LPS treated RAW 264.7 cells. IP: K562 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab219948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 50-55 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
IP
1/30.
Notes
WB
1/1000. Detects a band of approximately 50-55 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
IP
1/30.
Application notes
Is unsuitable for Flow Cyt.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on mouse thymus (PMID: 3472202, PMID:26926107) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on rat thymus (PMID: 3472202, PMID:26926107) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody [EPR22419] (ab219948)
    Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody [EPR22419] (ab219948)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling ETS2 with ab219948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in RAW 264.7 cells treated with lipopolysaccharide (500 ng/ml) for 6 hours. The nuclear counterstain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    Secondary antibody contril: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Negative control: No staining on rat cerebellum (PMID: 3472202) is observed. Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Negative control: No staining on mouse cerebrum (PMID: 3472202) is observed. Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-ETS2 antibody [EPR22419] (ab219948)
    Immunoprecipitation - Anti-ETS2 antibody [EPR22419] (ab219948)

    ETS2 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate with ab219948 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219948 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: K562 whole cell lysate 10 µg (Input).
    Lane 2: ab219948 IP in K562 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219948 in K562 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    The two immunoprecipitated bands represent non-phosphorylated and phosphorylated ETS2.

  • Western blot - Anti-ETS2 antibody [EPR22419] (ab219948)
    Western blot - Anti-ETS2 antibody [EPR22419] (ab219948)
    All lanes : Anti-ETS2 antibody [EPR22419] (ab219948) at 1/1000 dilution

    Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
    Lane 2 : Human thymus at 10 µg

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 26 seconds, Lane 2: 3 minutes.

    Lane 1 was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-ETS2 antibody [EPR22419] (ab219948)
    Western blot - Anti-ETS2 antibody [EPR22419] (ab219948)
    All lanes : Anti-ETS2 antibody [EPR22419] (ab219948) at 1/1000 dilution

    Lane 1 : Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : RAW 264.7 treated with 500ng/ml lipopolysaccharides(LPS) for 6 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 53 kDa
    Observed band size: 50-55 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 15590657). The doublets represent ETS2 and its phosphorylated form.

    This blot was developed using a higher sensitivity ECL substrate.

  • Anti-ETS2 antibody [EPR22419] (ab219948)
    Anti-ETS2 antibody [EPR22419] (ab219948)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab219948? Please let us know so that we can cite the reference in this datasheet.

ab219948 has not yet been referenced specifically in any publications.

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