Recombinant Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)](https://www.abcam.com/ps/products/126/ab126629/Images/ab126629-337403-anti-extracellular-matrix-protein-1-antibody-epr6701-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6701] to Extracellular matrix protein 1
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Extracellular matrix protein 1 antibody [EPR6701]
See all Extracellular matrix protein 1 primary antibodies -
Description
Rabbit monoclonal [EPR6701] to Extracellular matrix protein 1 -
Host species
Rabbit -
Specificity
The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.
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Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human Extracellular matrix protein 1 aa 100-200. The exact sequence is proprietary.
Database link: Q16610 -
Positive control
- WB: MDA MB 435 cell line, Rat heart, Rat kidney, Human skin cell, and A375 cell line lysates. IHC-P: Human breast carcinoma and Human kidney tissues. ICC/IF: A375 cells. IP: A375 cells. Flow Cyt (intra): A375 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.20 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6701 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
|
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
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| IP |
1/10 - 1/100.
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| IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat. |
| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/20. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa. |
|
IP
1/10 - 1/100. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat. |
|
ICC/IF
1/100 - 1/250. |
Target
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Function
Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. -
Tissue specificity
Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis. -
Involvement in disease
Defects in ECM1 are the cause of lipoid proteinosis (LiP) [MIM:247100]; also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1893 Human
- Entrez Gene: 116662 Rat
- Omim: 602201 Human
- SwissProt: Q16610 Human
- SwissProt: Q62894 Rat
- Unigene: 81071 Human
- Unigene: 97792 Rat
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Alternative names
- ECM 1 antibody
- Ecm1 antibody
- ECM1_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.01 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)](https://www.abcam.com/ps/products/126/ab126629/Images/ab126629-337535-anti-extracellular-matrix-protein-1-antibody-epr6701-western-blot.jpg)
Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)All lanes : Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) at 1/2000 dilution (Purified)
Lane 1 : A375 (Human malignant melanoma epithelial cell)
Lane 2 : Rat heart lysates
Lane 3 : Rat kidney lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa -
![Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)](https://www.abcam.com/ps/products/126/ab126629/Images/ab126629-337405-anti-extracellular-matrix-protein-1-antibody-epr6701-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)ab126629 (purified) at 1:20 dilution (2µg) immunoprecipitating Extracellular matrix protein 1 in A375 whole cell lysate.
Lane 1 (input): A375 (Human malignant melanoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab126629 & A375 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126629 in A375 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Immunocytochemistry/ Immunofluorescence - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)](https://www.abcam.com/ps/products/126/ab126629/Images/ab126629-337401-anti-extracellular-matrix-protein-1-antibody-epr6701-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)Immunocytochemistry/ Immunofluorescence analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)](https://www.abcam.com/ps/products/126/ab126629/Images/ab126629-337399-anti-extracellular-matrix-protein-1-antibody-epr6701-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)Intracellular Flow Cytometry analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)](https://www.abcam.com/ps/products/126/ab126629/Images/ab126629-140634-anti-extracellular-matrix-protein-1-antibody-epr6701-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)](https://www.abcam.com/ps/products/126/ab126629/Images/ab126629-140635-anti-extracellular-matrix-protein-1-antibody-epr6701-western-blot.jpg)
Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)All lanes : Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) at 1/1000 dilution
Lane 1 : MDA MB 435 cell line lysate
Lane 2 : Human skin cell lysate
Lane 3 : A375 cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibodty at 1/2000 dilution
Predicted band size: 61 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)](https://www.abcam.com/ps/products/126/ab126629/Images/ab126629-140633-anti-extracellular-matrix-protein-1-antibody-epr6701-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)](https://www.abcam.com/ps/products/126/ab126629/Images/ab126629-140632-anti-extracellular-matrix-protein-1-antibody-epr6701.jpg)
OI-RD Scanning - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) -
![Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)](https://www.abcam.com/ps/products/126/ab126629/Images/ab126629-10-benefits-of-recombinant-antibodies.png)
Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab126629 has been referenced in 5 publications.
- Yang H et al. MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1. Biosci Rep 40:N/A (2020). PubMed: 32401301
- Liu X et al. Characterization of seminal plasma proteomic alterations associated with the IVF and rescue-ICSI pregnancy in assisted reproduction. Andrology 8:407-420 (2020). PubMed: 31364287
- Li W et al. Exosomal FMR1-AS1 facilitates maintaining cancer stem-like cell dynamic equilibrium via TLR7/NF?B/c-Myc signaling in female esophageal carcinoma. Mol Cancer 18:22 (2019). PubMed: 30736860
- Fitzgerald HC et al. Idiopathic infertility in women is associated with distinct changes in proliferative phase uterine fluid proteins. Biol Reprod 98:752-764 (2018). PubMed: 29546322
- Ziegler YS et al. Integration of Breast Cancer Secretomes with Clinical Data Elucidates Potential Serum Markers for Disease Detection, Diagnosis, and Prognosis. PLoS One 11:e0158296 (2016). WB . PubMed: 27355404