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RecombinantRabMAb

Recombinant Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

Reviews (1)Q&A (2)References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
  • Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
  • Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
  • Immunocytochemistry/ Immunofluorescence - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
  • Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
  • Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
  • OI-RD Scanning - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
  • Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6701] to Extracellular matrix protein 1
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Extracellular matrix protein 1 antibody [EPR6701]
    See all Extracellular matrix protein 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6701] to Extracellular matrix protein 1

  • Host species

    Rabbit

  • Specificity

    The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human Extracellular matrix protein 1 aa 100-200. The exact sequence is proprietary.
    Database link: Q16610

  • Positive control

    • WB: MDA MB 435 cell line, Rat heart, Rat kidney, Human skin cell, and A375 cell line lysates. IHC-P: Human breast carcinoma and Human kidney tissues. ICC/IF: A375 cells. IP: A375 cells. Flow Cyt (intra): A375 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.
WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
IP
1/10 - 1/100.
IHC-P (1)
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.

See IHC antigen retrieval protocols.
ICC/IF
1/100 - 1/250.
Notes
Flow Cyt (Intra)
1/20.
WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.

See IHC antigen retrieval protocols.
ICC/IF
1/100 - 1/250.

Target

  • Function

    Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.

  • Tissue specificity

    Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis.

  • Involvement in disease

    Defects in ECM1 are the cause of lipoid proteinosis (LiP) [MIM:247100]; also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    • Alternative names

      • ECM 1 antibody
      • Ecm1 antibody
      • ECM1_HUMAN antibody
      • Extracellular matrix protein 1 antibody
      • Secretory component p85 antibody
      • URBWD antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.01 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      All lanes : Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) at 1/2000 dilution (Purified)

      Lane 1 : A375 (Human malignant melanoma epithelial cell)
      Lane 2 : Rat heart lysates
      Lane 3 : Rat kidney lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 61 kDa
      Observed band size: 61 kDa

    • Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

      ab126629 (purified) at 1:20 dilution (2µg) immunoprecipitating Extracellular matrix protein 1 in A375 whole cell lysate.
      Lane 1 (input): A375 (Human malignant melanoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab126629 & A375 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126629 in A375 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      Immunocytochemistry/ Immunofluorescence - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      Immunocytochemistry/ Immunofluorescence analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

      Intracellular Flow Cytometry analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

      ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      All lanes : Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) at 1/1000 dilution

      Lane 1 : MDA MB 435 cell line lysate
      Lane 2 : Human skin cell lysate
      Lane 3 : A375 cell line lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibodty at 1/2000 dilution

      Predicted band size: 61 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

      ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry.

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      OI-RD Scanning - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
      Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

    References (5)

    Publishing research using ab126629? Please let us know so that we can cite the reference in this datasheet.

    ab126629 has been referenced in 5 publications.

    • Yang H  et al. MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1. Biosci Rep 40:N/A (2020). PubMed: 32401301
    • Liu X  et al. Characterization of seminal plasma proteomic alterations associated with the IVF and rescue-ICSI pregnancy in assisted reproduction. Andrology 8:407-420 (2020). PubMed: 31364287
    • Li W  et al. Exosomal FMR1-AS1 facilitates maintaining cancer stem-like cell dynamic equilibrium via TLR7/NF?B/c-Myc signaling in female esophageal carcinoma. Mol Cancer 18:22 (2019). PubMed: 30736860
    • Fitzgerald HC  et al. Idiopathic infertility in women is associated with distinct changes in proliferative phase uterine fluid proteins. Biol Reprod 98:752-764 (2018). PubMed: 29546322
    • Ziegler YS  et al. Integration of Breast Cancer Secretomes with Clinical Data Elucidates Potential Serum Markers for Disease Detection, Diagnosis, and Prognosis. PLoS One 11:e0158296 (2016). WB . PubMed: 27355404

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Extracellular matrix protein 1 antibody [EPR6701]

     Below Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (spinal cord)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton-x
    Specification
    spinal cord
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 01 2017

    Question

    Do you have specific antibodies recognizing ECM1a and ECM1b independently working in WB or ELISA ?

    Read More
    Answer

    I have had a look at the nature of this protein: Extracellular matrix protein 1 (http://www.uniprot.org/uniprot/Q16610)

    Unfortunately, I do not think it is scientifically to detect only ECM1b without detecting ECM1a.

    The difference between these two isoforms is that ECM1b lacks amino acids 237-361. Therefore, if you had an antibody targeting this region, it would detect ECM1a but not ECM1b. You however wouldn't be able to specifically detect ECM1b as all the amino acids present in this isoform are also present in ECM1a.

    Read More

    Question

    Do you synthesize the anti-sera based on DNA sequence of proteins, if you do not, can you suggest me a company they make anti-sera, many thanks!

    Read More
    Answer

    Thank you for contacting us.

    In general, the selection of the immunogen varies from antibody to antibody. Some antibodies are raised against synthetic peptides (based ona protein sequence which was derived originally from a DNA sequence), some against protein fragments and others against the full length protein (either purified or overexpressed in another organism) or a cell preparation.

    Which specific antibody or protein target do you have in mind?

    As for the anti-IgA antibodies - the immunogendepends on the antibody.

    Please let me know more details about the products of your interest and I'd be happy to assist you further.

    Read More

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