Recombinant Anti-Fibronectin antibody [EPR23110-25] (ab268021)
![Western blot - Anti-Fibronectin antibody [EPR23110-25] (ab268021)](https://www.abcam.com/ps/products/268/ab268021/Images/ab268021-4-anti-fibronectin-antibody-epr23110-25-western-blot-human.jpg "Western blot - Anti-Fibronectin antibody [EPR23110-25] (ab268021)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23110-25] to Fibronectin
- Suitable for: IHC-P, IHC-Fr, Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Fibronectin antibody [EPR23110-25]
See all Fibronectin primary antibodies -
Description
Rabbit monoclonal [EPR23110-25] to Fibronectin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Fr, Flow Cyt, WBmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human serum, plasma, colon and stomach lysate. Mouse and rat plasma. Wild-type HAP1 whole cell lysate. IHC-P: Human mammary gland tissue. Human esophageal squamous cell carcinoma tissue. Mouse stomach and rat liver tissue. IHC-Fr: Mouse and rat colon tissue. Flow Cyt: NIH/3T3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23110-25 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab268021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| IHC-Fr |
1/100.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
| Flow Cyt |
1/500.
|
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| WB |
1/1000. Detects a band of approximately 285 kDa (predicted molecular weight: 262 kDa).
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| Notes |
|---|
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
1/100. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
Flow Cyt
1/500. |
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WB
1/1000. Detects a band of approximately 285 kDa (predicted molecular weight: 262 kDa). |
Target
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Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
Tissue specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
Involvement in disease
Glomerulopathy with fibronectin deposits 2 -
Sequence similarities
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
Developmental stage
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
Post-translational
modificationsSulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 2335 Human
- Entrez Gene: 14268 Mouse
- Entrez Gene: 25661 Rat
- Omim: 135600 Human
- SwissProt: P02751 Human
- SwissProt: P11276 Mouse
- SwissProt: P04937 Rat
- Unigene: 203717 Human
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Alternative names
- CIG antibody
- Cold insoluble globulin antibody
- Cold-insoluble globulin antibody
see all
Images
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Western blot - Anti-Fibronectin antibody [EPR23110-25] (ab268021)All lanes : Anti-Fibronectin antibody [EPR23110-25] (ab268021) at 1/1000 dilution
Lane 1 : Human colon lysate at 20 µg
Lane 2 : Human stomach lysate at 20 µg
Lane 3 : Wild-type HAP1 whole cell lysate at 40 µg
Lane 4 : Fibronectin knockout HAP1 whole cell lysate at 40 µg
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 262 kDa
Observed band size: 285 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 44 seconds.
The expression molecular weight observed is consistent with what has been described in the literature (PMID:28367057).
ab268021 was shown to specifically react with Fibronectin in wild-type HAP1 cells as signal was lost in Fibronectin knockout cells. Wild-type and Fibronectin knockout samples were subjected to SDS-PAGE. ab268021 and ab179475 (Rabbit anti-Integrin alpha V loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-25] (ab268021)](https://www.abcam.com/ps/products/268/ab268021/Images/ab268021-9-anti-fibronectin-antibody-epr23110-25-immunohistochemistry-liver-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-25] (ab268021)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Fibronectin with ab268021 at 1/2000 dilution (0.23 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the perivascular tissue of sinusoids of rat liver (PMID:650101). Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Frozen sections) - Anti-Fibronectin antibody [EPR23110-25] (ab268021)](https://www.abcam.com/ps/products/268/ab268021/Images/ab268021-2-anti-fibronectin-antibody-epr23110-25-immunohistochemistry-frozen-colon-rat.jpg)
Immunohistochemistry (Frozen sections) - Anti-Fibronectin antibody [EPR23110-25] (ab268021)Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat colon tissue labeling Fibronectin with ab268021 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Positive staining mainly on smooth muscle of rat colon (PMID: 17881565). The nuclear counterstain is DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Flow Cytometry - Anti-Fibronectin antibody [EPR23110-25] (ab268021)](https://www.abcam.com/ps/products/268/ab268021/Images/ab268021-5-anti-fibronectin-antibody-epr23110-25-flowcytometry-nih-3t3-mouse.jpg)
Flow Cytometry - Anti-Fibronectin antibody [EPR23110-25] (ab268021)Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling Fibronectin with ab268021 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-25] (ab268021)](https://www.abcam.com/ps/products/268/ab268021/Images/ab268021-8-anti-fibronectin-antibody-epr23110-25-immunohistochemistry-stomach-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-25] (ab268021)Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Fibronectin with ab268021 at 1/2000 dilution (0.23 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the smooth muscle of mouse stomach (PMID:650101). Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-25] (ab268021)](https://www.abcam.com/ps/products/268/ab268021/Images/ab268021-7-anti-fibronectin-antibody-epr23110-25-immunohistochemistry-esophageal-squamous-cell-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-25] (ab268021)Immunohistochemical analysis of paraffin-embedded human esophageal squamous cell carcinoma tissue labeling Fibronectin with ab268021 at 1/2000 dilution (0.23 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the stroma of human esophageal squamous cell carcinoma (PMID:30314454). Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-25] (ab268021)](https://www.abcam.com/ps/products/268/ab268021/Images/ab268021-6-anti-fibronectin-antibody-epr23110-25-immunohistochemistry-breast-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-25] (ab268021)Immunohistochemical analysis of paraffin-embedded human mammary gland tissue labeling Fibronectin with ab268021 at 1/2000 dilution (0.23 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the stroma of human mammary gland (PMID:650101). Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Western blot - Anti-Fibronectin antibody [EPR23110-25] (ab268021)](https://www.abcam.com/ps/products/268/ab268021/Images/ab268021-3-anti-fibronectin-antibody-epr23110-25-western-blot.jpg)
Western blot - Anti-Fibronectin antibody [EPR23110-25] (ab268021)All lanes : Anti-Fibronectin antibody [EPR23110-25] (ab268021) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lane 3 : Mouse plasma
Lane 4 : Rat plasma
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 262 kDa
Observed band size: 285 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3.25 seconds.
The expression molecular weight observed is consistent with what has been described in the literature (PMID:28367057).
-
![Immunohistochemistry (Frozen sections) - Anti-Fibronectin antibody [EPR23110-25] (ab268021)](https://www.abcam.com/ps/products/268/ab268021/Images/ab268021-1-anti-fibronectin-antibody-epr23110-25-immunohistochemistry-frozen-colon-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-Fibronectin antibody [EPR23110-25] (ab268021)Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse colon tissue labeling Fibronectin with ab268021 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Positive staining mainly on smooth muscle of mouse colon (PMID: 17881565). The nuclear counterstain is DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Anti-Fibronectin antibody [EPR23110-25] (ab268021)](https://www.abcam.com/ps/products/268/ab268021/Images/ab268021-10-benefits-of-recombinant-antibodies.png)
Anti-Fibronectin antibody [EPR23110-25] (ab268021)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab268021 has been referenced in 4 publications.
- Wang L et al. Combination CTLA-4 immunoglobulin treatment and ultrasound microbubble-mediated exposure improve renal function in a rat model of diabetic nephropathy. Aging (Albany NY) 13:8524-8540 (2021). PubMed: 33714204
- Li Q et al. Identification of the EMT-Related Genes Signature for Predicting Occurrence and Progression in Thyroid Cancer. Onco Targets Ther 14:3119-3131 (2021). PubMed: 34012269
- Li J et al. CircRNA TADA2A relieves idiopathic pulmonary fibrosis by inhibiting proliferation and activation of fibroblasts. Cell Death Dis 11:553 (2020). PubMed: 32694556
- Guo Y et al. SIRT1-modified human umbilical cord mesenchymal stem cells ameliorate experimental peritoneal fibrosis by inhibiting the TGF-ß/Smad3 pathway. Stem Cell Res Ther 11:362 (2020). PubMed: 32811535