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  1. Link

    fmo3-antibody-epr6968-ab126711.pdf

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RecombinantRabMAb

Recombinant Anti-FMO3 antibody [EPR6968] (ab126711)

  • Datasheet
  • SDS
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Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
  • Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
  • Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
  • Immunocytochemistry/ Immunofluorescence - Anti-FMO3 antibody [EPR6968] (ab126711)
  • OI-RD Scanning - Anti-FMO3 antibody [EPR6968] (ab126711)
  • Anti-FMO3 antibody [EPR6968] (ab126711)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6968] to FMO3
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-BDNF antibody [EPR1292] (ab108319)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Product image
Recombinant Human FMO3 protein (ab152391)

View more associated products

Overview

  • Product name

    Anti-FMO3 antibody [EPR6968]
    See all FMO3 primary antibodies
  • Description

    Rabbit monoclonal [EPR6968] to FMO3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FMO3 aa 300-400. The exact sequence is proprietary.
    Database link: P31513

  • Positive control

    • WB: Human fetal liver, human fetal kidney, mouse kidney, mouse liver, rat kidney and rat liver tissue lysates. IHC-P: Human, mouse and rat liver tissues. ICC/IF: HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.40 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6968
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-FMO3 antibody [EPR6968] - BSA and Azide free (ab232452)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human FMO3 protein (ab152391)
  • Related Products

    • Mouse liver tissue lysate - total protein (ab29301)
    • Mouse kidney normal tissue lysate - total protein (ab29305)
    • Mouse kidney tissue lysate - total protein (0 days) (ab7261)
    • Mouse kidney tissue lysate (14 days) (ab7262)
    • Mouse kidney tissue lysate (7 days) (ab7263)
    • Mouse liver tissue lysate - total protein (7 days) (ab7266)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/150.
WB (2)
1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa).

For unpurified use at 1/1000 - 1/10000.

IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
ICC/IF
1/150.
WB
1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa).

For unpurified use at 1/1000 - 1/10000.

IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO). Is also able to perform S-oxidation when acting on sulfide compounds.
  • Tissue specificity

    Liver.
  • Involvement in disease

    Defects in FMO3 are the cause of trimethylaminuria (TMAU) [MIM:602079]; also known as fish-odor syndrome. TMAU is an inborn error of metabolism associated with an offensive body odor and caused by deficiency of FMO-mediated N-oxidation of amino-trimethylamine (TMA) derived from foodstuffs. Such individuals excrete relatively large amounts of TMA in their urine, sweat, and breath, and exhibit a fishy body odor characteristic of the malodorous free amine.
  • Sequence similarities

    Belongs to the FMO family.
  • Cellular localization

    Microsome membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession P31513 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2328 Human
    • Entrez Gene: 14262 Mouse
    • Entrez Gene: 84493 Rat
    • Omim: 136132 Human
    • SwissProt: P31513 Human
    • SwissProt: P97501 Mouse
    • SwissProt: Q9EQ76 Rat
    • Unigene: 445350 Human
    • Unigene: 2900 Mouse
    • Unigene: 163228 Rat
    see all
  • Alternative names

    • Dimethylaniline monooxygenase [N oxide forming] 3 antibody
    • Dimethylaniline monooxygenase [N-oxide-forming] 3 antibody
    • Dimethylaniline monooxygenase 3 antibody
    • Dimethylaniline oxidase 3 antibody
    • dJ127D3.1 antibody
    • Flavin containing monooxygenase 3 antibody
    • FMO 3 antibody
    • FMO form 2 antibody
    • FMO II antibody
    • FMO3 antibody
    • FMO3_HUMAN antibody
    • FMOII antibody
    • Hepatic flavin containing monooxygenase 3 antibody
    • Hepatic flavin-containing monooxygenase 3 antibody
    • MGC34400 antibody
    • TMAU antibody
    • Trimethylamine monooxygenase antibody
    see all

Images

  • Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
    Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
    All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/5000 dilution (purified)

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Human fetal liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
    Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
    All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/5000 dilution (purified)

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
    Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)
    All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)

    Immunohistochemitsy (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling FMO3 with unpurified ab126711 at a 1/500 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FMO3 antibody [EPR6968] (ab126711)
    Immunocytochemistry/ Immunofluorescence - Anti-FMO3 antibody [EPR6968] (ab126711)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling FMO3 with purified ab126711 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • OI-RD Scanning - Anti-FMO3 antibody [EPR6968] (ab126711)
    OI-RD Scanning - Anti-FMO3 antibody [EPR6968] (ab126711)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-FMO3 antibody [EPR6968] (ab126711)
    Anti-FMO3 antibody [EPR6968] (ab126711)

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab126711? Please let us know so that we can cite the reference in this datasheet.

ab126711 has been referenced in 5 publications.

  • Huang S  et al. Flavin-Containing Monooxygenases Are Conserved Regulators of Stress Resistance and Metabolism. Front Cell Dev Biol 9:630188 (2021). PubMed: 33644069
  • Bai S  et al. Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype. Front Mol Biosci 8:650604 (2021). PubMed: 34277700
  • Li L  et al. Fructus Ligustri Lucidi preserves bone quality through the regulation of gut microbiota diversity, oxidative stress, TMAO and Sirt6 levels in aging mice. Aging (Albany NY) 11:9348-9368 (2019). PubMed: 31715585
  • Astafev AA  et al. Calorie restriction effects on circadian rhythms in gene expression are sex dependent. Sci Rep 7:9716 (2017). PubMed: 28851928
  • Liao BM  et al. Flavin-Containing Monooxygenase 3 Reduces Endoplasmic Reticulum Stress in Lipid-Treated Hepatocytes. Mol Endocrinol 30:417-28 (2016). PubMed: 26886171

Customer reviews and Q&As

Show All Reviews Q&A
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1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-FMO3 antibody [EPR6968]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Huh7)
Permeabilization
No
Specification
Huh7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 08 2016

Western blot abreview for Anti-FMO3 antibody [EPR6968]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Gel Running Conditions
Non-reduced Denaturing (8%)
Loading amount
30 µg
Specification
primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 mins as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 16 2016

Western blot abreview for Anti-FMO3 antibody [EPR6968]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
65 µg
Gel Running Conditions
Reduced Denaturing (Novex 4-12% Bis-Tris midi-gel)
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Treatment
500ug/kg TCDD for 6hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 10 2014

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