Name: Anti-FMO3 antibody [EPR6968]
Brand: RabMAb
SKU: ab126711
Rating: 4 (3 reviews)

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Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6968] to FMO3
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-FMO3 antibody [EPR6968]
See all FMO3 primary antibodies
Description

Rabbit monoclonal [EPR6968] to FMO3
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human FMO3 aa 300-400. The exact sequence is proprietary.
Database link: P31513
Positive control
- WB: Human fetal liver, human fetal kidney, mouse kidney, mouse liver, rat kidney and rat liver tissue lysates. IHC-P: Human, mouse and rat liver tissues. ICC/IF: HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 2.40 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6968
Isotype

IgG
Research areas

Alternative Versions
- Anti-FMO3 antibody [EPR6968] - BSA and Azide free (ab232452)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human FMO3 protein (ab152391)
Related Products

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF	(1)	1/150.
WB	(2)	1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa). For unpurified use at 1/1000 - 1/10000.
IHC-P		1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
ICC/IF 1/150.
WB 1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa). For unpurified use at 1/1000 - 1/10000.
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Function

Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO). Is also able to perform S-oxidation when acting on sulfide compounds.
Tissue specificity

Liver.
Involvement in disease

Defects in FMO3 are the cause of trimethylaminuria (TMAU) [MIM:602079]; also known as fish-odor syndrome. TMAU is an inborn error of metabolism associated with an offensive body odor and caused by deficiency of FMO-mediated N-oxidation of amino-trimethylamine (TMA) derived from foodstuffs. Such individuals excrete relatively large amounts of TMA in their urine, sweat, and breath, and exhibit a fishy body odor characteristic of the malodorous free amine.
Sequence similarities

Belongs to the FMO family.
Cellular localization

Microsome membrane. Endoplasmic reticulum membrane.
Target information above from: UniProt accession P31513 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2328 Human
- Entrez Gene: 14262 Mouse
- Entrez Gene: 84493 Rat
- Omim: 136132 Human
- SwissProt: P31513 Human
- SwissProt: P97501 Mouse
- SwissProt: Q9EQ76 Rat
- Unigene: 445350 Human
- Unigene: 2900 Mouse
- Unigene: 163228 Rat
see all
Alternative names
- Dimethylaniline monooxygenase [N oxide forming] 3 antibody
- Dimethylaniline monooxygenase [N-oxide-forming] 3 antibody
- Dimethylaniline monooxygenase 3 antibody
- Dimethylaniline oxidase 3 antibody
- dJ127D3.1 antibody
- Flavin containing monooxygenase 3 antibody
- FMO 3 antibody
- FMO form 2 antibody
- FMO II antibody
- FMO3 antibody
- FMO3_HUMAN antibody
- FMOII antibody
- Hepatic flavin containing monooxygenase 3 antibody
- Hepatic flavin-containing monooxygenase 3 antibody
- MGC34400 antibody
- TMAU antibody
- Trimethylamine monooxygenase antibody
see all

Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)

All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/5000 dilution (purified)

Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)

All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/5000 dilution (purified)

Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-FMO3 antibody [EPR6968] (ab126711)

All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/1000 dilution (unpurified)

Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal kidney tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)

Immunohistochemitsy (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling FMO3 with unpurified ab126711 at a 1/500 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-FMO3 antibody [EPR6968] (ab126711)

Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling FMO3 with purified ab126711 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
OI-RD Scanning - Anti-FMO3 antibody [EPR6968] (ab126711)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-FMO3 antibody [EPR6968] (ab126711)

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab126711? Please let us know so that we can cite the reference in this datasheet.

ab126711 has been referenced in 5 publications.

Huang S et al. Flavin-Containing Monooxygenases Are Conserved Regulators of Stress Resistance and Metabolism. Front Cell Dev Biol 9:630188 (2021). PubMed: 33644069
Bai S et al. Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype. Front Mol Biosci 8:650604 (2021). PubMed: 34277700
Li L et al. Fructus Ligustri Lucidi preserves bone quality through the regulation of gut microbiota diversity, oxidative stress, TMAO and Sirt6 levels in aging mice. Aging (Albany NY) 11:9348-9368 (2019). PubMed: 31715585
Astafev AA et al. Calorie restriction effects on circadian rhythms in gene expression are sex dependent. Sci Rep 7:9716 (2017). PubMed: 28851928
Liao BM et al. Flavin-Containing Monooxygenase 3 Reduces Endoplasmic Reticulum Stress in Lipid-Treated Hepatocytes. Mol Endocrinol 30:417-28 (2016). PubMed: 26886171

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Huh7)

Permeabilization

Specification

Huh7

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 08 2016

Application

Western blot

Sample

Mouse Cell lysate - whole cell (primary hepatoctyes)

Gel Running Conditions

Non-reduced Denaturing (8%)

Loading amount

30 µg

Specification

primary hepatoctyes

Blocking step

I-Block(Applied biosystems) as blocking agent for 30 mins as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 16 2016

Application

Western blot

Loading amount

65 µg

Gel Running Conditions

Reduced Denaturing (Novex 4-12% Bis-Tris midi-gel)

Sample

Mouse Tissue lysate - whole (liver)

Specification

liver

Treatment

500ug/kg TCDD for 6hrs

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 10 2014

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (5)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-FMO3 antibody [EPR6968]

Western blot abreview for Anti-FMO3 antibody [EPR6968]

Western blot abreview for Anti-FMO3 antibody [EPR6968]

Dissociation constant (K_D)