Name: Anti-FUBP1/FBP antibody [EPR12327]
Brand: RabMAb
SKU: ab181111
Rating: 5 (2 reviews)

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Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12327] to FUBP1/FBP
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-FUBP1/FBP antibody [EPR12327]
See all FUBP1/FBP primary antibodies
Description

Rabbit monoclonal [EPR12327] to FUBP1/FBP
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat, HeLa, Raji and HepG2 whole cell lysate (ab7900), rat brain, mouse brain and mouse spleen tissue lysates. IHC-P: Human breast carcinoma and pancreas tissues. ICC/IF: HeLa and HepG2 cells. Flow Cyt (intra): Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12327
Isotype

IgG
Research areas

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/60 - 1/90. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB		1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 68 kDa). For unpurified use at 1/1000 - 1/20000.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF	(1)	1/250 - 1/500.

Notes
Flow Cyt (Intra) 1/60 - 1/90. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 68 kDa). For unpurified use at 1/1000 - 1/20000.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF 1/250 - 1/500.

Function

Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
Sequence similarities

Contains 4 KH domains.
Post-translational
modifications

Ubiquitinated. This targets the protein for proteasome-mediated degradation.
Cellular localization

Nucleus.
Target information above from: UniProt accession Q96AE4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 8880 Human
- Entrez Gene: 51886 Mouse
- Entrez Gene: 654496 Rat
- Omim: 603444 Human
- SwissProt: Q96AE4 Human
- SwissProt: Q91WJ8 Mouse
- SwissProt: Q32PX7 Rat
- Unigene: 567380 Human
- Unigene: 278922 Mouse
- Unigene: 177078 Rat
see all
Alternative names
- DNA helicase V antibody
- far upstream element (FUSE) binding protein 1 antibody
- Far upstream element (FUSE) binding protein 4 antibody
- Far upstream element binding protein 1 antibody
- far upstream element binding protein antibody
- Far upstream element-binding protein 1 antibody
- FBP antibody
- FUBP antibody
- Fubp1 antibody
- FUBP1_HUMAN antibody
- Fubp4 antibody
- FUSE binding protein 1 antibody
- FUSE-binding protein 1 antibody
- HDH V antibody
see all

Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

All lanes : Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/2000 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

All lanes : Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/20000 dilution (purified)

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FUBP1/FBP with purified ab181111 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling FUBP1/FBP with purified ab181111 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.

Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
Flow Cytometry (Intracellular) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Intracellular Flow Cytometry analysis of Jurkat cells labelling FUBP1 / FBP with purified ab181111 at 1/60 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/5000 dilution (unpurified) + Jurkat cell lysate at 20 µg

Secondary
Goat anti-rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

All lanes : Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/20000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling FUBP1/FBP with unpurified ab181111 at a dilution of 1/500 followed by prediluted HRP Polymer for rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labeling FUBP1/FBP with unpurified ab181111 at a dilution of 1/500 followed by prediluted HRP Polymer for rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Immunocytochemistry/Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling FUBP1/FBP with unpurified ab181111 at 1/500 dilution (red) followed by Alexa Fluor^® 555-conjugated goat anti rabbit IgG secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Flow Cytometry (Intracellular) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixed Jurkat cells labeling FUBP1/FBP with unpurified ab181111 at a dilution of 1/90 (pink), compared to a rabbit monoclonal IgG isotype control (green), followed by goat anti rabbit IgG (FITC) secondary antibody at a dilution of 1/150.
Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab181111? Please let us know so that we can cite the reference in this datasheet.

ab181111 has been referenced in 10 publications.

Yang B et al. TNPO1-Mediated Nuclear Import of FUBP1 Contributes to Tumor Immune Evasion by Increasing NRP1 Expression in Cervical Cancer. J Immunol Res 2021:9994004 (2021). PubMed: 33987449
Wang H et al. Protein and Signaling Pathway Responses to rhIL-6 Intervention Before Lobaplatin Treatment in Osteosarcoma Cells. Front Oncol 11:602712 (2021). PubMed: 33791202
Zhang XX et al. Long non-coding RNA small nucleolar RNA host gene 6 aggravates pancreatic cancer through upregulation of far upstream element binding protein 1 by sponging microRNA-26a-5p. Chin Med J (Engl) 133:1211-1220 (2020). PubMed: 32433053
Kang M et al. Multiple Functions of Fubp1 in Cell Cycle Progression and Cell Survival. Cells 9:N/A (2020). PubMed: 32481602
Xiong X et al. GSK343 induces programmed cell death through the inhibition of EZH2 and FBP1 in osteosarcoma cells. Cancer Biol Ther 21:213-222 (2020). PubMed: 31651209
Zheng Y et al. FUBP1 and FUBP2 enforce distinct epigenetic setpoints for MYC expression in primary single murine cells. Commun Biol 3:545 (2020). PubMed: 33005010
Elman JS et al. Identification of FUBP1 as a Long Tail Cancer Driver and Widespread Regulator of Tumor Suppressor and Oncogene Alternative Splicing. Cell Rep 28:3435-3449.e5 (2019). PubMed: 31553912
Frost JR et al. Adenovirus 5 E1A-Mediated Suppression of p53 via FUBP1. J Virol 92:N/A (2018). PubMed: 29743362
Seiler M et al. Somatic Mutational Landscape of Splicing Factor Genes and Their Functional Consequences across 33 Cancer Types. Cell Rep 23:282-296.e4 (2018). WB ; Human . PubMed: 29617667
Wang J et al. A novel long intergenic noncoding RNA indispensable for the cleavage of mouse two-cell embryos. EMBO Rep 17:1452-1470 (2016). PubMed: 27496889

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1-2 of 2 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Brain neural stem cell)

Permeabilization

Yes - 0.2% Triton X 100

Specification

Brain neural stem cell

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Apr 22 2015

Application

Immunoprecipitation

Sample

Mouse Cell lysate - whole cell (Brain neural stem cell)

Total protein in input

300 µg

Immuno-precipitation step

Protein G

Specification

Brain neural stem cell

Abcam user community

Verified customer

Submitted Apr 22 2015

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (10)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-FUBP1/FBP antibody [EPR12327]

Immunoprecipitation abreview for Anti-FUBP1 / FBP antibody [EPR12327]

Post-translational
modifications