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  1. Link

    fubp1fbp-antibody-epr12327-ab181111.pdf

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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription Other
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RecombinantRabMAb

Recombinant Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (2) Submit a question References (10)

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Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
  • Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
  • Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
  • Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
  • Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
  • Flow Cytometry (Intracellular) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
  • Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
  • Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
  • Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
  • Flow Cytometry (Intracellular) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
  • Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12327] to FUBP1/FBP
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

APC Carrier Free HRP PE

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FFN206, fluorescent VMAT2 substrate (ab144554)
Protein
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Recombinant Human FUBP1/FBP protein (ab152933)
Primary
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Anti-Met (c-Met) antibody [EP1454Y] - BSA and Azide free (ab157370)

View more associated products

Overview

  • Product name

    Anti-FUBP1/FBP antibody [EPR12327]
    See all FUBP1/FBP primary antibodies
  • Description

    Rabbit monoclonal [EPR12327] to FUBP1/FBP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, HeLa, Raji and HepG2 whole cell lysate (ab7900), rat brain, mouse brain and mouse spleen tissue lysates. IHC-P: Human breast carcinoma and pancreas tissues. ICC/IF: HeLa and HepG2 cells. Flow Cyt (intra): Jurkat cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12327
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Alternative Versions

    • Anti-FUBP1/FBP antibody [EPR12327] - BSA and Azide free (ab240239)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • Mouse spleen tissue lysate - total protein (ab29293)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • Jurkat whole cell lysate (ab30128)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (0 days) (ab7188)
    • Mouse brain tissue lysate - total protein (14 days) (ab7189)
    • Mouse brain tissue lysate - total protein (7 days) (ab7190)
    • Mouse spleen tissue lysate - total protein (14 days) (ab7274)
    • Jurkat whole cell lysate (ab7899)
    • Mouse small intestine tissue lysate - total protein (ab7939)
  • Recombinant Protein

    • Recombinant Human FUBP1/FBP protein (ab152933)
  • Related Products

    • Recombinant Human FUBP1/FBP protein (ab152933)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/60 - 1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 68 kDa).

For unpurified use at 1/1000 - 1/20000.

IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF (1)
1/250 - 1/500.
Notes
Flow Cyt (Intra)
1/60 - 1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 68 kDa).

For unpurified use at 1/1000 - 1/20000.

IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF
1/250 - 1/500.

Target

  • Function

    Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
  • Sequence similarities

    Contains 4 KH domains.
  • Post-translational
    modifications

    Ubiquitinated. This targets the protein for proteasome-mediated degradation.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q96AE4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8880 Human
    • Entrez Gene: 51886 Mouse
    • Entrez Gene: 654496 Rat
    • Omim: 603444 Human
    • SwissProt: Q96AE4 Human
    • SwissProt: Q91WJ8 Mouse
    • SwissProt: Q32PX7 Rat
    • Unigene: 567380 Human
    • Unigene: 278922 Mouse
    • Unigene: 177078 Rat
    see all
  • Alternative names

    • DNA helicase V antibody
    • far upstream element (FUSE) binding protein 1 antibody
    • Far upstream element (FUSE) binding protein 4 antibody
    • Far upstream element binding protein 1 antibody
    • far upstream element binding protein antibody
    • Far upstream element-binding protein 1 antibody
    • FBP antibody
    • FUBP antibody
    • Fubp1 antibody
    • FUBP1_HUMAN antibody
    • Fubp4 antibody
    • FUSE binding protein 1 antibody
    • FUSE-binding protein 1 antibody
    • HDH V antibody
    see all

Images

  • Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    All lanes : Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/2000 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 74 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 74 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 74 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    All lanes : Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/20000 dilution (purified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 74 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FUBP1/FBP with purified ab181111 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling FUBP1/FBP with purified ab181111 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Flow Cytometry (Intracellular) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Flow Cytometry (Intracellular) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

    Intracellular Flow Cytometry analysis of Jurkat cells labelling FUBP1 / FBP with purified ab181111 at 1/60 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

     

     

  • Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/5000 dilution (unpurified) + Jurkat cell lysate at 20 µg

    Secondary
    Goat anti-rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 74 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    All lanes : Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/20000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 74 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling FUBP1/FBP with unpurified ab181111 at a dilution of 1/500 followed by prediluted HRP Polymer for rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labeling FUBP1/FBP with unpurified ab181111 at a dilution of 1/500 followed by prediluted HRP Polymer for rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

    Immunocytochemistry/Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling FUBP1/FBP with unpurified ab181111 at 1/500 dilution (red) followed by Alexa Fluor® 555-conjugated goat anti rabbit IgG secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Flow Cytometry (Intracellular) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Flow Cytometry (Intracellular) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

    Intracellular flow cytometric analysis of2% paraformaldehyde-fixed Jurkat cells labeling FUBP1/FBP with unpurified ab181111 at a dilution of 1/90 (pink), compared to a rabbit monoclonal IgG isotype control (green), followed by goat anti rabbit IgG (FITC) secondary antibody at a dilution of 1/150. 

     

     

  • Anti-FUBP1/FBP antibody [EPR12327] (ab181111)
    Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (10)

Publishing research using ab181111? Please let us know so that we can cite the reference in this datasheet.

ab181111 has been referenced in 10 publications.

  • Yang B  et al. TNPO1-Mediated Nuclear Import of FUBP1 Contributes to Tumor Immune Evasion by Increasing NRP1 Expression in Cervical Cancer. J Immunol Res 2021:9994004 (2021). PubMed: 33987449
  • Wang H  et al. Protein and Signaling Pathway Responses to rhIL-6 Intervention Before Lobaplatin Treatment in Osteosarcoma Cells. Front Oncol 11:602712 (2021). PubMed: 33791202
  • Zhang XX  et al. Long non-coding RNA small nucleolar RNA host gene 6 aggravates pancreatic cancer through upregulation of far upstream element binding protein 1 by sponging microRNA-26a-5p. Chin Med J (Engl) 133:1211-1220 (2020). PubMed: 32433053
  • Kang M  et al. Multiple Functions of Fubp1 in Cell Cycle Progression and Cell Survival. Cells 9:N/A (2020). PubMed: 32481602
  • Xiong X  et al. GSK343 induces programmed cell death through the inhibition of EZH2 and FBP1 in osteosarcoma cells. Cancer Biol Ther 21:213-222 (2020). PubMed: 31651209
  • Zheng Y  et al. FUBP1 and FUBP2 enforce distinct epigenetic setpoints for MYC expression in primary single murine cells. Commun Biol 3:545 (2020). PubMed: 33005010
  • Elman JS  et al. Identification of FUBP1 as a Long Tail Cancer Driver and Widespread Regulator of Tumor Suppressor and Oncogene Alternative Splicing. Cell Rep 28:3435-3449.e5 (2019). PubMed: 31553912
  • Frost JR  et al. Adenovirus 5 E1A-Mediated Suppression of p53 via FUBP1. J Virol 92:N/A (2018). PubMed: 29743362
  • Seiler M  et al. Somatic Mutational Landscape of Splicing Factor Genes and Their Functional Consequences across 33 Cancer Types. Cell Rep 23:282-296.e4 (2018). WB ; Human . PubMed: 29617667
  • Wang J  et al. A novel long intergenic noncoding RNA indispensable for the cleavage of mouse two-cell embryos. EMBO Rep 17:1452-1470 (2016). PubMed: 27496889

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-FUBP1/FBP antibody [EPR12327]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain neural stem cell)
Permeabilization
Yes - 0.2% Triton X 100
Specification
Brain neural stem cell
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 22 2015

Immunoprecipitation abreview for Anti-FUBP1 / FBP antibody [EPR12327]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Brain neural stem cell)
Total protein in input
300 µg
Immuno-precipitation step
Protein G
Specification
Brain neural stem cell
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 22 2015

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