Name: Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]
Brand: RabMAb
SKU: ab109364
Rating: 4 (10 reviews)

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Immunocytochemistry/ Immunofluorescence - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4805] to GABARAP+GABARAPL1+GABARAPL2
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

APC Carrier Free HRP PE

Product name

Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]
See all GABARAP+GABARAPL1+GABARAPL2 primary antibodies
Description

Rabbit monoclonal [EPR4805] to GABARAP+GABARAPL1+GABARAPL2
Host species

Rabbit
Specificity

ab109364 will also recognize related targets GABARAPL1 and GABARAPL2: GABARAPL1: pBLAST 100% immunogen homology AND Abreview 38385 WB data GABARAPL2: pBLAST 93% homology (only 1aa gap of 14aa) AND Abreview 38385 WB data
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Predicted to work with: Silk worm
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Fetal kidney lysate, fetal brain lysate, SH-SY5Y cell lysate, HepG2 whole cell lysate (ab7900), human brain tissue, HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4805
Isotype

IgG
Research areas

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/20.
WB	(5)	1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P	(1)	1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Antigen retrieval is recommended. Ssee IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
ICC/IF	(1)	1/100 - 1/500.

Notes
Flow Cyt (Intra) 1/20.
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Antigen retrieval is recommended. Ssee IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
ICC/IF 1/100 - 1/500.

Application notes

Is unsuitable for IP.

Relevance

GABARAP: Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
GABARAPL1: Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
GABARAPL2: Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Database links
- SwissProt: O95166 Human
- SwissProt: P60520 Human
- SwissProt: Q9H0R8 Human

Immunocytochemistry/ Immunofluorescence - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)

Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling GABARAP+GABARAPL1+GABARAPL2 with purified ab109364 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling GABARAP+GABARAPL1+GABARAPL2 with Purified ab109364 at 1:500 dilution (0.29 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Flow Cytometry (Intracellular) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GABARAP+GABARAPL1+GABARAPL2 with purified ab109364 at 1/20 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)

Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364) at 1/5000 dilution (purified) + Rat kidney lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)

All lanes : Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364) at 1/5000 dilution (purified)

Lane 1 : Mouse kidney lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST
Flow Cytometry (Intracellular) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)

Unpurified ab109364 staining GABARAP+GABARAPL1+GABARAPL2 in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluorr^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black)

Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)

All lanes : Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364) at 1/1000 dilution (unpurified)

Lane 1 : Fetal kidney lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Fetal brain lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 14 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)

Unpurified ab109364, at 1/500, staining GABARAP+GABARAPL1+GABARAPL2 in Human brain tissue by immunohistochemistry.
Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab109364? Please let us know so that we can cite the reference in this datasheet.

ab109364 has been referenced in 35 publications.

Kohrs FE et al. Systematic functional analysis of rab GTPases reveals limits of neuronal robustness to environmental challenges in flies. Elife 10:N/A (2021). PubMed: 33666175
Liu Y et al. GABARAP suppresses EMT and breast cancer progression via the AKT/mTOR signaling pathway. Aging (Albany NY) 13:5858-5874 (2021). PubMed: 33591943
Yu S et al. Rab5 and Rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in Drosophila. Elife 10:N/A (2021). PubMed: 33560224
He L et al. DNAJB12 and Hsp70 triage arrested intermediates of N1303K-CFTR for endoplasmic reticulum-associated autophagy. Mol Biol Cell 32:538-553 (2021). PubMed: 33534640
Wang LJ et al. Drosophila MICOS knockdown impairs mitochondrial structure and function and promotes mitophagy in muscle tissue. Biol Open 9:N/A (2020). PubMed: 33268479

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Customer reviews and Q&As

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1-10 of 13 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Melanoma cells)

Permeabilization

Specification

Melanoma cells

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Fixative

Acetone

Abcam user community

Verified customer

Submitted Feb 11 2021

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Liver)

Permeabilization

Specification

Liver

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 23°C

Fixative

Acetone

Abcam user community

Verified customer

Submitted Feb 03 2021

Application

Immunocytochemistry

Sample

Human Cultured Cells (Melanoma cells)

Permeabilization

Specification

Melanoma cells

Blocking step

Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: RT°C

Fixative

Acetone

Abcam user community

Verified customer

Submitted Dec 05 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: EDTA

Permeabilization

Specification

Brain

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 07 2020

Application

Western blot

Sample

Drosophila melanogaster Tissue lysate - whole (whole fly)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

20 µg

Specification

whole fly

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 31 2018

Application

Western blot

Sample

Human Cell lysate - whole cell (HepG2 cells)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

HepG2 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Jan 13 2017

Application

Western blot

Sample

Human Tissue lysate - whole (skeletal muscle tissue)

Gel Running Conditions

Reduced Denaturing (4-12%)

Loading amount

20 µg

Specification

skeletal muscle tissue

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 12 2015

Application

Western blot

Sample

Silk worm Tissue lysate - whole (Midgut)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

40 µg

Specification

Midgut

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 02 2014

Application

Western blot

Loading amount

30 µg

Gel Running Conditions

Reduced Denaturing (13%)

Sample

Human Cell lysate - whole cell (SH-SY5Y cells stably expressing GFP, GFP-GABARAP,)

Specification

SH-SY5Y cells stably expressing GFP, GFP-GABARAP,

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. George Allen

Verified customer

Submitted Sep 26 2013

Application

IHC - Wholemount

Sample

Fruit fly (Drosophila melanogaster) Tissue (fat body)

Specification

fat body

Abcam user community

Verified customer

Submitted Sep 19 2013

1-10 of 13 Abreviews or Q&A

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Relevance

Database links

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (35)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

Immunohistochemistry (Frozen sections) abreview for Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

Immunocytochemistry abreview for Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

Western blot abreview for Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

Western blot abreview for Anti-GABARAP antibody [EPR4805]

Western blot abreview for Anti-GABARAP antibody [EPR4805]

Western blot abreview for Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

Western blot abreview for Anti-GABARAP antibody [EPR4805]

IHC - Wholemount abreview for Anti-GABARAP antibody [EPR4805]