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  1. Link

    gart-antibody-epr11622-ab169550.pdf

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Signal Transduction Metabolism Amino Acids
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RecombinantRabMAb

Recombinant Anti-GART antibody [EPR11622] (ab169550)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

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Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)
  • Western blot - Anti-GART antibody [EPR11622] (ab169550)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)
  • Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)
  • Western blot - Anti-GART antibody [EPR11622] (ab169550)
  • Anti-GART antibody [EPR11622] (ab169550)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11622] to GART
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-GART antibody [EPR11622]
    See all GART primary antibodies
  • Description

    Rabbit monoclonal [EPR11622] to GART
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human GART.

  • Positive control

    • HeLa, HepG2, K562 and A431 cell lysates; Human colonic adenocarcinoma and tonsil tissues; HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11622
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Alternative Versions

    • Anti-GART antibody [EPR11622] - BSA and Azide free (ab249500)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab7909)
    • K-562 whole cell lysate (ab7911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/100 - 1/500.
WB
1/1000 - 1/5000. Predicted molecular weight: 107 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
1/100 - 1/500.
WB
1/1000 - 1/5000. Predicted molecular weight: 107 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Pathway

    Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.
    Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.
    Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
  • Sequence similarities

    In the N-terminal section; belongs to the GARS family.
    In the central section; belongs to the AIR synthase family.
    In the C-terminal section; belongs to the GART family.
    Contains 1 ATP-grasp domain.
  • Target information above from: UniProt accession P22102 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2618 Human
    • Entrez Gene: 14450 Mouse
    • Entrez Gene: 288259 Rat
    • Omim: 138440 Human
    • SwissProt: P22102 Human
    • SwissProt: Q64737 Mouse
    • Unigene: 473648 Human
    • Unigene: 10281 Mouse
    • Unigene: 4505 Mouse
    see all
  • Alternative names

    • 5'-phosphoribosylglycinamide transformylase antibody
    • AIR synthase antibody
    • AIRS antibody
    • GAR transformylase antibody
    • GARS antibody
    • GART antibody
    • GARTF antibody
    • Glycinamide ribonucleotide synthetase antibody
    • MGC47764 antibody
    • PAIS antibody
    • PGFT antibody
    • Phosphoribosyl-aminoimidazole synthetase antibody
    • Phosphoribosylglycinamide formyltransferase antibody
    • Phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase antibody
    • Phosphoribosylglycinamide formyltransferase, EC 2.1.2.29 antibody
    • Phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase antibody
    • Phosphoribosylglycinamide synthetase antibody
    • PRGS antibody
    • PUR2_HUMAN antibody
    • Trifunctional purine biosynthetic protein adenosine 3 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)
    Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)This image is courtesy of an Abreview submitted by Kirk McManus

    ab169550 staining GART in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

    See Abreview

  • Western blot - Anti-GART antibody [EPR11622] (ab169550)
    Western blot - Anti-GART antibody [EPR11622] (ab169550)
    All lanes : Anti-GART antibody [EPR11622] (ab169550) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 107 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)

    Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling GART with ab169550 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling GART with ab169550 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)
    Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)
    Immunofluorescent analysis of HepG2 cells labeling GART with ab169550 at 1/100 dilution.
  • Western blot - Anti-GART antibody [EPR11622] (ab169550)
    Western blot - Anti-GART antibody [EPR11622] (ab169550)
    Anti-GART antibody [EPR11622] (ab169550) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate

    Secondary
    HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

    Predicted band size: 107 kDa

  • Anti-GART antibody [EPR11622] (ab169550)
    Anti-GART antibody [EPR11622] (ab169550)

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab169550? Please let us know so that we can cite the reference in this datasheet.

ab169550 has been referenced in 3 publications.

  • Thompson LL  et al. Reduced SKP1 Expression Induces Chromosome Instability through Aberrant Cyclin E1 Protein Turnover. Cancers (Basel) 12:N/A (2020). PubMed: 32106628
  • Sun S  et al. Overexpressed microRNA-103a-3p inhibits acute lower-extremity deep venous thrombosis via inhibition of CXCL12. IUBMB Life 72:492-504 (2020). PubMed: 31613419
  • Song W  et al. Edoxaban improves venous thrombosis via increasing hydrogen sulfide and homocysteine in rat model. Mol Med Rep 16:7706-7714 (2017). PubMed: 28944880

Customer reviews and Q&As

Show All Reviews Q&A
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Immunocytochemistry/ Immunofluorescence abreview for Anti-GART antibody [EPR11622]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

Submitted Jan 23 2014

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