RecombinantRabMAb

Recombinant Anti-GGH antibody [EPR8014] (ab138495)

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Western blot - Anti-GGH antibody [EPR8014] (ab138495)
  • OI-RD Scanning - Anti-GGH antibody [EPR8014] (ab138495)
  • Anti-GGH antibody [EPR8014] (ab138495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8014] to GGH
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-GGH antibody [EPR8014]
    See all GGH primary antibodies

  • Description

    Rabbit monoclonal [EPR8014] to GGH

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human GGH aa 250-350. The exact sequence is proprietary.

  • Positive control

    • MCF7, HepG2 and HeLa cell lysates

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab138495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.

  • Sequence similarities

    Belongs to the peptidase C26 family.
    Contains 1 gamma-glutamyl hydrolase domain.

  • Cellular localization

    Secreted > extracellular space. Lysosome. Melanosome. While its intracellular location is primarily the lysosome, most of the enzyme activity is secreted. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    • Alternative names

      • Conjugase antibody
      • folylpolygammaglutamyl hydrolase antibody
      • gamma glutamyl hydrolase antibody
      • Gamma-Glu-X carboxypeptidase antibody
      • Gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) antibody
      • Gamma-glutamyl hydrolase antibody
      • GGH antibody
      • GGH_HUMAN antibody
      • gH antibody
      see all

    Images

    • Western blot - Anti-GGH antibody [EPR8014] (ab138495)
      Western blot - Anti-GGH antibody [EPR8014] (ab138495)
      All lanes : Anti-GGH antibody [EPR8014] (ab138495) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 36 kDa

    • OI-RD Scanning - Anti-GGH antibody [EPR8014] (ab138495)
      OI-RD Scanning - Anti-GGH antibody [EPR8014] (ab138495)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-GGH antibody [EPR8014] (ab138495)
      Anti-GGH antibody [EPR8014] (ab138495)

    References (0)

    Publishing research using ab138495? Please let us know so that we can cite the reference in this datasheet.

    ab138495 has not yet been referenced specifically in any publications.

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