Recombinant Anti-GGH antibody [EPR8014] (ab138495)
![Western blot - Anti-GGH antibody [EPR8014] (ab138495)](https://www.abcam.com/ps/products/138/ab138495/Images/ab138495-153764-anti-glutamyl-hydrolase-gamma-antibody-epr8014-western-blot.jpg "Western blot - Anti-GGH antibody [EPR8014] (ab138495)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8014] to GGH
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GGH antibody [EPR8014]
See all GGH primary antibodies -
Description
Rabbit monoclonal [EPR8014] to GGH -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human GGH aa 250-350. The exact sequence is proprietary.
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Positive control
- MCF7, HepG2 and HeLa cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Dissociation constant (KD)
KD = 3.78 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8014 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa. |
Target
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Function
Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. -
Sequence similarities
Belongs to the peptidase C26 family.
Contains 1 gamma-glutamyl hydrolase domain. -
Cellular localization
Secreted > extracellular space. Lysosome. Melanosome. While its intracellular location is primarily the lysosome, most of the enzyme activity is secreted. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 8836 Human
- Omim: 601509 Human
- SwissProt: Q92820 Human
- Unigene: 78619 Human
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Alternative names
- Conjugase antibody
- folylpolygammaglutamyl hydrolase antibody
- gamma glutamyl hydrolase antibody
see all
Images
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Western blot - Anti-GGH antibody [EPR8014] (ab138495)All lanes : Anti-GGH antibody [EPR8014] (ab138495) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa -
![OI-RD Scanning - Anti-GGH antibody [EPR8014] (ab138495)](https://www.abcam.com/ps/products/138/ab138495/Images/ab138495-153763-anti-glutamyl-hydrolase-gamma-antibody-epr8014.jpg)
OI-RD Scanning - Anti-GGH antibody [EPR8014] (ab138495) -
![Anti-GGH antibody [EPR8014] (ab138495)](https://www.abcam.com/ps/products/138/ab138495/Images/ab138495-3-benefits-of-recombinant-antibodies.png)
Anti-GGH antibody [EPR8014] (ab138495)
Datasheets and documents
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Datasheet download
References (0)
ab138495 has not yet been referenced specifically in any publications.
