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  1. Link

    irg1-antibody-epr22066-ab222411.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Anti-IRG1 antibody [EPR22066] (ab222411)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (4) Submit a question References (5)

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Immunoprecipitation - Anti-IRG1 antibody [EPR22066] (ab222411)
  • Western blot - Anti-IRG1 antibody [EPR22066] (ab222411)
  • Anti-IRG1 antibody [EPR22066] (ab222411)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22066] to IRG1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-IRG1 antibody [EPR22066]
    See all IRG1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22066] to IRG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours) and THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS after 52 hours) whole cell lysates. IP: THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS added for 16 hours) whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22066
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-IRG1 antibody [EPR22066] - BSA and Azide free (ab238169)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab222411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa).
IP
1/30.
Notes
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa).
IP
1/30.

Target

  • Sequence similarities

    Belongs to the prpD family.
  • Target information above from: UniProt accession A6NK06 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 730249 Human
    • Entrez Gene: 16365 Mouse
    • Omim: 615275 Human
    • SwissProt: A6NK06 Human
    • SwissProt: P54987 Mouse
    • Alternative names

      • ACOD1 antibody
      • Aconitate decarboxylase 1 antibody
      • Aconitate decarboxylase antibody
      • CAD antibody
      • Cis aconitate decarboxylase antibody
      • Immune responsive gene 1 protein homolog antibody
      • Immune-responsive gene 1 protein homolog antibody
      • Immunoresponsive 1 homolog (mouse) antibody
      • Irg1 antibody
      • IRG1_HUMAN antibody
      • OC116 antibody
      • OPTB1 antibody
      • TCTIRC7 antibody
      see all

    Images

    • Immunoprecipitation - Anti-IRG1 antibody [EPR22066] (ab222411)
      Immunoprecipitation - Anti-IRG1 antibody [EPR22066] (ab222411)

      IRG1 was immunoprecipitated from 0.35 mg of THP-1 (human monocytic leukemia cell line)(treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 μg/ml LPS added for 16 hours) whole cell lysate with ab222411 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

      Lane 1: THP-1 (treated with PMA/LPS) whole cell lysate, 10 μg (Input).
      Lane 2: ab222411 IP in THP-1 (treated with PMA/LPS) whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222411 in THP-1 (treated with PMA/LPS) whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 8 seconds.

    • Western blot - Anti-IRG1 antibody [EPR22066] (ab222411)
      Western blot - Anti-IRG1 antibody [EPR22066] (ab222411)
      All lanes : Anti-IRG1 antibody [EPR22066] (ab222411) at 1/1000 dilution

      Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 2 : RAW 264.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours) whole cell lysate
      Lane 3 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
      Lane 4 : THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS after 52 hours) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 53 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure times.

      Lanes 1 & 2: 3 minutes.

      Lanes 3 & 4: 8 seconds.

      The molecular mass observed is consistent with what has been described in the literature (PMID: 23610393 and PMID: 28699638).
      The protein is up-regulated after lipopolysaccharide (LPS) stimulation (PMID: 25640654 and PMID: 26679997).

    • Anti-IRG1 antibody [EPR22066] (ab222411)
      Anti-IRG1 antibody [EPR22066] (ab222411)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (5)

    Publishing research using ab222411? Please let us know so that we can cite the reference in this datasheet.

    ab222411 has been referenced in 5 publications.

    • Tomlinson KL  et al. Staphylococcus aureus induces an itaconate-dominated immunometabolic response that drives biofilm formation. Nat Commun 12:1399 (2021). PubMed: 33658521
    • Azzimato V  et al. Hepatic miR-144 Drives Fumarase Activity Preventing NRF2 Activation During Obesity. Gastroenterology 161:1982-1997.e11 (2021). PubMed: 34425095
    • Riquelme SA  et al. Pseudomonas aeruginosa Utilizes Host-Derived Itaconate to Redirect Its Metabolism to Promote Biofilm Formation. Cell Metab 31:1091-1106.e6 (2020). PubMed: 32428444
    • Hooftman A  et al. The Immunomodulatory Metabolite Itaconate Modifies NLRP3 and Inhibits Inflammasome Activation. Cell Metab 32:468-478.e7 (2020). PubMed: 32791101
    • Li Y  et al. 4-Octyl Itaconate Alleviates Lipopolysaccharide-Induced Acute Lung Injury in Mice by Inhibiting Oxidative Stress and Inflammation. Drug Des Devel Ther 14:5547-5558 (2020). PubMed: 33364751

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Flow Cytometry abreview for Anti-IRG1 antibody [EPR22066]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Bone-marrow-derived macrophage)
    Permeabilization
    Yes - Fixation/Permeabilization solution
    Gating Strategy
    Alive/Singlets cells
    Specification
    Bone-marrow-derived macrophage
    Preparation
    Cell harvesting/tissue preparation method: cell scraping
    Sample buffer: PBS/0.2%BSA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Aurélie Poli

    Verified customer

    Submitted Nov 03 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IRG1 antibody [EPR22066]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (adipose tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.1% sodium azide
    Permeabilization
    No
    Specification
    adipose tissue
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 10 2021

    Western blot abreview for Anti-IRG1 antibody [EPR22066]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (BMDM)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    BMDM
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Yuanxin Yang

    Verified customer

    Submitted Jan 27 2021

    Western blot abreview for Anti-IRG1 antibody [EPR22066]

    Inconclusive
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (spinal cord)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    30 µg
    Specification
    spinal cord
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    qingyu

    Verified customer

    Submitted Sep 25 2019

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