Recombinant Anti-IRG1 antibody [EPR22066] (ab222411)
![Immunoprecipitation - Anti-IRG1 antibody [EPR22066] (ab222411)](https://www.abcam.com/ps/products/222/ab222411/Images/ab222411-315931-anti-irg1-antibody-epr22066-immunoprecipitation.jpg "Immunoprecipitation - Anti-IRG1 antibody [EPR22066] (ab222411)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22066] to IRG1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-IRG1 antibody [EPR22066]
See all IRG1 primary antibodies -
Description
Rabbit monoclonal [EPR22066] to IRG1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: RAW 264.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours) and THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS after 52 hours) whole cell lysates. IP: THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS added for 16 hours) whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22066 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa).
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| IP |
1/30.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa). |
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IP
1/30. |
Target
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Sequence similarities
Belongs to the prpD family. - Information by UniProt
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Database links
- Entrez Gene: 730249 Human
- Entrez Gene: 16365 Mouse
- Omim: 615275 Human
- SwissProt: A6NK06 Human
- SwissProt: P54987 Mouse
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Alternative names
- ACOD1 antibody
- Aconitate decarboxylase 1 antibody
- Aconitate decarboxylase antibody
see all
Images
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Immunoprecipitation - Anti-IRG1 antibody [EPR22066] (ab222411)
IRG1 was immunoprecipitated from 0.35 mg of THP-1 (human monocytic leukemia cell line)(treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 μg/ml LPS added for 16 hours) whole cell lysate with ab222411 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: THP-1 (treated with PMA/LPS) whole cell lysate, 10 μg (Input).
Lane 2: ab222411 IP in THP-1 (treated with PMA/LPS) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222411 in THP-1 (treated with PMA/LPS) whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds. -
![Western blot - Anti-IRG1 antibody [EPR22066] (ab222411)](https://www.abcam.com/ps/products/222/ab222411/Images/ab222411-315930-anti-irg1-antibody-epr22066-western-blot.jpg)
Western blot - Anti-IRG1 antibody [EPR22066] (ab222411)All lanes : Anti-IRG1 antibody [EPR22066] (ab222411) at 1/1000 dilution
Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours) whole cell lysate
Lane 3 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 4 : THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS after 52 hours) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lanes 1 & 2: 3 minutes.
Lanes 3 & 4: 8 seconds.
The molecular mass observed is consistent with what has been described in the literature (PMID: 23610393 and PMID: 28699638).
The protein is up-regulated after lipopolysaccharide (LPS) stimulation (PMID: 25640654 and PMID: 26679997). -
![Anti-IRG1 antibody [EPR22066] (ab222411)](https://www.abcam.com/ps/products/222/ab222411/Images/ab222411-3-benefits-of-recombinant-antibodies.png)
Anti-IRG1 antibody [EPR22066] (ab222411)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab222411 has been referenced in 5 publications.
- Tomlinson KL et al. Staphylococcus aureus induces an itaconate-dominated immunometabolic response that drives biofilm formation. Nat Commun 12:1399 (2021). PubMed: 33658521
- Azzimato V et al. Hepatic miR-144 Drives Fumarase Activity Preventing NRF2 Activation During Obesity. Gastroenterology 161:1982-1997.e11 (2021). PubMed: 34425095
- Riquelme SA et al. Pseudomonas aeruginosa Utilizes Host-Derived Itaconate to Redirect Its Metabolism to Promote Biofilm Formation. Cell Metab 31:1091-1106.e6 (2020). PubMed: 32428444
- Hooftman A et al. The Immunomodulatory Metabolite Itaconate Modifies NLRP3 and Inhibits Inflammasome Activation. Cell Metab 32:468-478.e7 (2020). PubMed: 32791101
- Li Y et al. 4-Octyl Itaconate Alleviates Lipopolysaccharide-Induced Acute Lung Injury in Mice by Inhibiting Oxidative Stress and Inflammation. Drug Des Devel Ther 14:5547-5558 (2020). PubMed: 33364751