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    jagged1-antibody-epr4290-ab109536.pdf

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Cardiovascular Angiogenesis Inhibitors
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Jagged1 antibody [EPR4290] (ab109536)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (41)

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Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
  • Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
  • Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
  • Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
  • Anti-Jagged1 antibody [EPR4290] (ab109536)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4290] to Jagged1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-Jagged1 antibody [EPR4290]
    See all Jagged1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4290] to Jagged1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and HUVEC whole cell lysate, NIH:OVCAR-3 cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4290
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Inhibitors
    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules

Associated products

  • Alternative Versions

    • Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Jagged1 protein (Fc Chimera Active) (ab108575)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 134 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 134 kDa.
Application notes
Is unsuitable for ICC/IF or IHC-P.

Target

  • Function

    Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
  • Tissue specificity

    Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
  • Involvement in disease

    Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
    Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
  • Sequence similarities

    Contains 1 DSL domain.
    Contains 15 EGF-like domains.
  • Developmental stage

    Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P78504 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 182 Human
    • Entrez Gene: 16449 Mouse
    • Entrez Gene: 29146 Rat
    • Omim: 601920 Human
    • SwissProt: P78504 Human
    • SwissProt: Q9QXX0 Mouse
    • SwissProt: Q63722 Rat
    • Unigene: 224012 Human
    • Unigene: 22398 Mouse
    • Unigene: 88804 Rat
    see all
  • Alternative names

    • AGS antibody
    • AHD antibody
    • AWS antibody
    • CD 339 antibody
    • CD339 antibody
    • CD339 antigen antibody
    • Headturner antibody
    • hJ1 antibody
    • Htu antibody
    • Jag 1 antibody
    • Jag1 antibody
    • JAG1_HUMAN antibody
    • Jagged 1 antibody
    • Jagged1 (Alagille syndrome) antibody
    • Jagged1 antibody
    • JAGL1 antibody
    • MGC104644 antibody
    • OTTHUMP00000030278 antibody
    • Protein jagged-1 antibody
    • Ser 1 antibody
    • Ser1 antibody
    • Serrate 1 antibody
    • Slalom antibody
    see all

Images

  • Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 2 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 4 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 134 kDa
    Observed band size: 200, 23 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM /TBST

    Negative controls: Jurkat and MOLT-4 whole cell lysate (PMID: 30231940)

    200-kDa full length and 23-kDa C-terminal JAG1 are observed. The molecular weights are consistent with what has been described in the literature (PMID: 30890522, 30890522).

    This blot was developed using a high sensitivity ECL substrate.

  • Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : JAG1 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 134 kDa
    Observed band size: 200 kDa why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab109536 observed at 200 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab109536 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in JAG1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and JAG1 knockout samples were subjected to SDS-PAGE. Ab109536 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/1000 dilution (Purified) + HepG2 at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 134 kDa
    Observed band size: 200 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution (Unpurified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : NIH:OVCAR-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 134 kDa
    Observed band size: 200 kDa why is the actual band size different from the predicted?

  • Anti-Jagged1 antibody [EPR4290] (ab109536)
    Anti-Jagged1 antibody [EPR4290] (ab109536)

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (41)

Publishing research using ab109536? Please let us know so that we can cite the reference in this datasheet.

ab109536 has been referenced in 41 publications.

  • Han G  et al. MicroRNA-598 inhibits the growth of triple negative breast cancer cells by targeting JAG1. Exp Ther Med 21:235 (2021). PubMed: 33603843
  • Hu W  et al. Ethanol Extracted from Radix of Actinidia Chinensis Inhibits Human Colon Tumor Through Inhibiting Notch-signaling Pathway. J Cancer 12:622-629 (2021). PubMed: 33403022
  • Zhu Z  et al. NAT10 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Mediating N4-Acetylcytidine Modification of Gremlin 1. Stem Cells Int 2021:8833527 (2021). PubMed: 33953754
  • Geles KG  et al. NOTCH3-targeted antibody drug conjugates regress tumors by inducing apoptosis in receptor cells and through transendocytosis into ligand cells. Cell Rep Med 2:100279 (2021). PubMed: 34095881
  • Tao S  et al. Linc00514 promotes breast cancer metastasis and M2 polarization of tumor-associated macrophages via Jagged1-mediated notch signaling pathway. J Exp Clin Cancer Res 39:191 (2020). PubMed: 32943090
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-Jagged1 antibody [EPR4290]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Lizard Tissue lysate - whole (liver)
Gel Running Conditions
Reduced Denaturing (4-20%, mini-PROTEAN TGX Gel)
Loading amount
120 µg
Specification
liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Oliver Griffith

Verified customer

Submitted Oct 03 2019

Immunohistochemistry (Frozen sections) abreview for Anti-Jagged1 antibody [EPR4290]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Hamster Tissue sections (10-day old ovary)
Permeabilization
No
Specification
10-day old ovary
Blocking step
10% donkey serum/glycine/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

PROF. Shyamal Roy

Verified customer

Submitted Apr 01 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Jagged1 antibody [EPR4290]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
Permeabilization
No
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 29 2018

Western blot abreview for Anti-Jagged1 antibody [EPR4290]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Sample
Human Cell lysate - whole cell (non small cell lung cancer)
Specification
non small cell lung cancer
Treatment
siRNA treated
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 07 2014

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