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  1. Link

    jnk1-phospho-y185--jnk2-phospho-y185--jnk3-phospho-y223-antibody-ep1597y-ab76572.pdf

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RecombinantRabMAb

Recombinant Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (42)

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Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
  • Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
  • Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
  • Dot Blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
  • Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1597Y] to JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223)
  • Suitable for: Dot blot, WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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View more associated products

Overview

  • Product name

    Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y]
  • Description

    Rabbit monoclonal [EP1597Y] to JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223)
  • Host species

    Rabbit
  • Specificity

    Detects JNK1 (pY185), JNK2 (pY185) and JNK3 (pY223).

  • Tested applications

    Suitable for: Dot blot, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HeLa cell lysate; WB: Lysate from UV-treated HeLa, HEK-293, NIH/3T3, and C6 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1597Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • MAPK pathway
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
    • Cancer
    • Tumor biomarkers
    • Oncoproteins
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Alternative Versions

    • Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] - BSA and Az (ab239886)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
Use at an assay dependent concentration.
WB (1)
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

For unpurified use at 1/5000 - 1/20000.

IP
1/70.

For unpurified use at 1/20.

Notes
Dot blot
Use at an assay dependent concentration.
WB
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

For unpurified use at 1/5000 - 1/20000.

IP
1/70.

For unpurified use at 1/20.

Target

  • Cellular localization

    JNK3: Cytoplasm.
  • Database links

    • Entrez Gene: 5599 Human
    • Entrez Gene: 5601 Human
    • Entrez Gene: 5602 Human
    • Entrez Gene: 26414 Mouse
    • Entrez Gene: 26419 Mouse
    • Entrez Gene: 26420 Mouse
    • Entrez Gene: 116554 Rat
    • Entrez Gene: 25272 Rat
    • Entrez Gene: 50658 Rat
    • Omim: 601158 Human
    • Omim: 602896 Human
    • Omim: 602897 Human
    • SwissProt: P45983 Human
    • SwissProt: P45984 Human
    • SwissProt: P53779 Human
    • SwissProt: Q61831 Mouse
    • SwissProt: Q91Y86 Mouse
    • SwissProt: Q9WTU6 Mouse
    • SwissProt: P49185 Rat
    • SwissProt: P49186 Rat
    • SwissProt: P49187 Rat
    • Unigene: 125503 Human
    • Unigene: 138211 Human
    • Unigene: 484371 Human
    • Unigene: 21495 Mouse
    • Unigene: 39253 Mouse
    • Unigene: 472459 Mouse
    • Unigene: 68933 Mouse
    • Unigene: 4090 Rat
    • Unigene: 9910 Rat
    • Unigene: 9911 Rat
    see all

Images

  • Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    Lanes 1-6 : Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572) at 1/5000 dilution (Purified)


    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) treated with 200J/m2 UV-C then recovery for 30 minutes whole cell lysates
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates
    Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 6 : C6 (Rat glial tumor glial cell) treated with 40J/m2 UV-C then recovery for 30 minutes whole cell lysates


    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution


    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

    Purified ab76572 at 1/70 dilution (2ug) immunoprecipitating JNK1+JNK2+JNK3 in HeLa treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate (10µg)
    Lane 2 (+): ab76572 + HeLa treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76572 in HeLa treated with 20J/m2 UV-C then recovery for 1h whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 46, 54 kDa

  • Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    All lanes : Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572) at 1/2000 dilution (unpurified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) Whole cell lysates with 5% NFDM/TBST
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates with 5% NFDM/TBST
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour, whole cell lysates. Then the membrane was incubated with alkaline phosphatase with 5% NFDM/TBST
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates. Then the membrane was incubated with lambda phosphatase with 5% NFDM/TBST

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Dot Blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    Dot Blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

    Lane 1: Human JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) phospho peptide
    Lane 2: Human JNK1+JNK2+JNK3 non-phospho pepetide

    Stained with unpurified ab76572 at a 1:1000 dilution. Dection was with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051)  at a 1:100,000 dilution.

    Exposure: 3 minutes

  • Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (42)

Publishing research using ab76572? Please let us know so that we can cite the reference in this datasheet.

ab76572 has been referenced in 42 publications.

  • Hou TY  et al. JNK and p38 Inhibitors Prevent Transforming Growth Factor-ß1-Induced Myofibroblast Transdifferentiation in Human Graves' Orbital Fibroblasts. Int J Mol Sci 22:N/A (2021). PubMed: 33799469
  • Lee EC  et al. The Histone Deacetylase Inhibitor (MS-275) Promotes Differentiation of Human Dental Pulp Stem Cells into Odontoblast-Like Cells Independent of the MAPK Signaling System. Int J Mol Sci 21:N/A (2020). PubMed: 32796747
  • Tong W  et al. Resveratrol inhibits LPS-induced inflammation through suppressing the signaling cascades of TLR4-NF-?B/MAPKs/IRF3. Exp Ther Med 19:1824-1834 (2020). PubMed: 32104238
  • Zhao TM  et al. Bicyclol Attenuates Acute Liver Injury by Activating Autophagy, Anti-Oxidative and Anti-Inflammatory Capabilities in Mice. Front Pharmacol 11:463 (2020). PubMed: 32362825
  • Jeong JH  et al. A New TGF-ß1 Inhibitor, CTI-82, Antagonizes Epithelial-Mesenchymal Transition through Inhibition of Phospho-SMAD2/3 and Phospho-ERK. Biology (Basel) 9:N/A (2020). PubMed: 32605257
View all Publications for this product

Customer reviews and Q&As

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Western blot abreview for Anti-JNK1 + JNK2 + JNK1 + JNK2 + JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary rat hepatocyte)
Gel Running Conditions
Reduced Denaturing (9%)
Loading amount
20 µg
Treatment
1mM paracetamol
Specification
Primary rat hepatocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Dr. Philip Probert

Verified customer

Submitted Jun 03 2016

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