RecombinantRabMAb

Recombinant Anti-Lipoprotein a antibody [EPR19731] (ab208184)

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Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
  • Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
  • Anti-Lipoprotein a antibody [EPR19731] (ab208184)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19731] to Lipoprotein a
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Lipoprotein a antibody [EPR19731]
    See all Lipoprotein a primary antibodies

  • Description

    Rabbit monoclonal [EPR19731] to Lipoprotein a

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human serum, plasma, liver, blood and adipose lysates. IHC-P: Human liver and glioma tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab208184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 100-500 kDa (predicted molecular weight: 501 kDa).
Notes
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 100-500 kDa (predicted molecular weight: 501 kDa).

Target

  • Relevance

    Lipoprotein(a) (Lp(a)) is a lipoprotein subclass assembled in the blood from low density lipoprotein (LDL) molecules and apolipoprotein-a (apo-a). Lp(a) recruits inflammatory cells through interaction with Mac-1 integrin. High Lp(a) in blood is a risk factor for coronary heart disease, cerebrovascular disease, atherosclerosis, thrombosis, and stroke. Lp(a) concentrations may be affected by disease states, but are only moderately affected by diet, exercise and other environmental factors. Lipid-reducing drugs have no effect on Lp(a) concentration. High Lp(a) predicts risk of early atherosclerosis similar to high LDL, but in advanced atherosclerosis, Lp(a) is a risk factor independent of LDL, indicating a coagulant risk of plaque thrombosis. Apo(a) contains domains that are very similar to plasminogen (PLG). Lp(a) accumulates in the vessel wall and inhibits binding of PLG to the cell surface, reducing plasmin generation which increases clotting. This inhibition also promotes proliferation of smooth muscle cells. These unique features of Lp(a) suggest a role in the generation of clots and atherosclerosis.

  • Database links

    • Alternative names

      • AK38 antibody
      • Antiangiogenic AK38 protein antibody
      • apo(a) antibody
      • APOA antibody
      • apolipoprotein(a) antibody
      • LP antibody
      • lp(a) antibody
      • LPA antibody
      see all

    Images

    • Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
      Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
      All lanes : Anti-Lipoprotein a antibody [EPR19731] (ab208184) at 1/5000 dilution

      Lane 1 : Human serum
      Lane 2 : Human plasma
      Lane 3 : Human liver lysate
      Lane 4 : Human blood
      Lane 5 : Human adipose lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 501 kDa
      Observed band size: 100-500 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The expression profile observed is consistent with what has been described in the literature (PMID: 9590382, 10484779 and 10703674).

    • Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
      Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
      All lanes : Anti-Lipoprotein a antibody [EPR19731] (ab208184) at 1/5000 dilution

      Lane 1 : Human serum
      Lane 2 : Human plasma
      Lane 3 : Human liver lysate
      Lane 4 : Human blood
      Lane 5 : Human adipose lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 501 kDa
      Observed band size: 100-500 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The expression profile observed is consistent with what has been described in the literature (PMID: 9590382, 10484779 and 10703674).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (ab208184)

      Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Lipoprotein a with ab208184 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human liver is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (ab208184)

      Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling Lipoprotein a with ab208184 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on blood vessels of human glioma is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-Lipoprotein a antibody [EPR19731] (ab208184)
      Anti-Lipoprotein a antibody [EPR19731] (ab208184)

    References (0)

    Publishing research using ab208184? Please let us know so that we can cite the reference in this datasheet.

    ab208184 has not yet been referenced specifically in any publications.

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